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Over-expression of GmKR3, a TIR–NBS–LRR type R gene, confers resistance to multiple viruses in soybean

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That overexpression of GmKR3 enhances innate virus resistance by stimulating.

Abstract

Soybean mosaic virus (SMV) is found in many soybean production areas, and SMV infection is one of the prevalent viral diseases that can cause significant yield losses in soybean. In plants, resistance (R) genes are involved in pathogen reorganization and innate immune response activation. Most R proteins have nucleotide-binding site and leucine-rich repeat (NBS–LRR) domains, and some of the NBS–LRR type R proteins in dicots have Toll/Interleukin-1 Receptor (TIR) motifs. We report here the analysis of the over-expression of GmKR3, a soybean TIR–NBS–LRR type R gene on virus resistance in soybean. When over-expressed in soybean, GmKR3 enhanced the plant’s resistance to several strains of SMV, the closely related potyviruses bean common mosaic virus (BCMV) and watermelon mosaic virus (WMV), and the secovirus bean pod mottle virus (BPMV). Importantly, over-expression of GmKR3 did not affect plant growth and development, including yield and qualities of the seeds. HPLC analysis showed that abscisic acid (ABA) content increased in the 35S:GmKR3 transgenic plants, and in both wild-type and 35S:GmKR3 transgenic plants in response to virus inoculation. Consistent with this observation, we found that the expression of two ABA catabolism genes was down-regulated in 35S:GmKR3 transgenic plants. We also found that the expression of Gm04.3, an ABA responsive gene encoding BURP domain-containing protein, was up-regulated in 35S:GmKR3 transgenic plants. Taken together, our results suggest that overexpression of GmKR3 enhanced virus resistance in soybean, which was achieved at least in part via ABA signaling.

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Acknowledgements

We thank Dr. Haijian Zhi (Nanjing Agricultural University) for providing the virus strains. This work was supported by the National Key R&D Program of China (2016YFD0101902), the China National Novel Transgenic Organisms Breeding Project (2016ZX08004-004) and the Programme for Introducing Talents to Universities (B07017). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Author notes

  1. Hongwei Xun and Xiangdong Yang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Molecular Epigenetics of MOE & Institute of Genetics and Cytology, Northeast Normal University, Changchun, China

    Hongwei Xun, Meng Wang, Peng Guo, Ying Wang, Jinsong Pang, Shucai Wang & Bao Liu

  2. Jilin Academy of Agricultural Sciences, Changchun, 130033, China

    Xiangdong Yang, Hongli He & Yingshan Dong

  3. Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China

    Xianzhong Feng

  4. College of Life Science, Linyi University, Linyi, China

    Shucai Wang

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  1. Hongwei Xun
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  5. Peng Guo
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  6. Ying Wang
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  7. Jinsong Pang
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  8. Yingshan Dong
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  10. Shucai Wang
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  11. Bao Liu
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Contributions

BL, SW, XF, YD and JP designed the research. HX, XY, HH, MW, PG and YW performed the experiments, HX, XY, JP and BL analyzed the data, HX and SW drafted the manuscript, and all authors participated in the revision of the manuscript.

Corresponding authors

Correspondence to Jinsong Pang, Yingshan Dong or Shucai Wang.

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On behalf of all authors, the corresponding authors state that there is no conflict of interest.

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Xun, H., Yang, X., He, H. et al. Over-expression of GmKR3, a TIR–NBS–LRR type R gene, confers resistance to multiple viruses in soybean. Plant Mol Biol 99, 95–111 (2019). https://doi.org/10.1007/s11103-018-0804-z

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  • DOI: https://doi.org/10.1007/s11103-018-0804-z

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