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Overexpression of a peroxidase gene (AtPrx64) of Arabidopsis thaliana in tobacco improves plant’s tolerance to aluminum stress

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AtPrx64 is one of the peroxidases gene up-regulated in Al stress and has some functions in the formation of plant second cell wall. Its overexpression may improve plant tolerance to Al by some ways. Studies on its function under Al stress may help us to understand the mechanism of plant tolerance to Al stress.

Abstract

In Arabidopsis thaliana, the expressions of some genes (AtPrxs) encoding class III plant peroxidases have been found to be either up-regulated or down-regulated under aluminum (Al) stress. Among 73 genes that encode AtPrxs in Arabidopsis, AtPrx64 is always up-regulated by Al stress, suggesting this gene plays protective roles in response to such stress. In this study, transgenic tobacco plants were generated to examine the effects of overexpressing of AtPrx64 gene on the tolerance to Al stress. The results showed that overexpression of AtPrx64 gene increased the root growth and reduced the accumulation of Al and ROS in the roots. Compared with wild type controls, transgenic tobaccos had much less soluble proteins and malondialdehyde in roots and much more root citrate exudation. The activity of plasma membrane (PM) H+-ATPase, the phosphorylation of PM H+-ATPase and its interaction with 14-3-3 proteins increased in transgenic tobaccos; moreover, the content of lignin in root tips also increased. Taken together, these results showed that overexpression of AtPrx64 gene might enhance the tolerance of tobacco to Al stress.

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Acknowledgements

This work was supported in part by the National Natural Science Foundation of China (No. 31260297, 31560351), the analysis and testing foundation of Kunming University of Science and Technology (20150736).

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    1. Faculty of Environmental Science and Engineering, Chenggong Campus, Kunming University of Science and Technology, Kunming, 650500, China

      Yuanshuang Wu

    2. Faculty of Life Science and Technology, Chenggong Campus, Kunming University of Science and Technology, Kunming, 650500, China

      Yuanshuang Wu, Zhili Yang, Jingyi How, Huini Xu, Limei Chen & Kunzhi Li

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    1. Yuanshuang Wu
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    Contributions

    KL and LC designed the research, YW and ZY performed most of the research, JH and HX performed part of the preparations of plant material, ZY and YW analyzed the data, YW and KL contributed to the discussion and wrote the article.

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    Correspondence to Kunzhi Li.

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    Yuanshuang Wu and Zhili Yang have contributed equally to this work.

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    Wu, Y., Yang, Z., How, J. et al. Overexpression of a peroxidase gene (AtPrx64) of Arabidopsis thaliana in tobacco improves plant’s tolerance to aluminum stress. Plant Mol Biol 95, 157–168 (2017). https://doi.org/10.1007/s11103-017-0644-2

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