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The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice

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Key message

This is the first report that GLP gene (OsGLP2-1) is involved in panicle blast and bacterial blight resistance in rice. In addition to its resistance to blast and bacterial blight, OsGLP2-1 has also been reported to co-localize with a QTLs for sheath blight resistance in rice. These suggest that the disease resistance provided by OsGLP2-1 is quantitative and broad spectrum. Its good resistance to these major diseases in rice makes it to be a promising target in rice breeding.

Abstract

Rice (Oryza sativa) blast caused by Magnaporthe oryzae and bacterial blight caused by Xanthomonas oryzae pv. oryzae are the two most destructive rice diseases worldwide. Germin-like protein (GLP) gene family is one of the important defense gene families which have been reported to be involved in disease resistance in plants. Although GLP proteins have been demonstrated to positively regulate leaf blast resistance in rice, their involvement in resistance to panicle blast and bacterial blight, has not been reported. In this study, we reported that one of the rice GLP genes, OsGLP2-1, was significantly induced by blast fungus. Overexpression of OsGLP2-1 quantitatively enhanced resistance to leaf blast, panicle blast and bacterial blight. The temporal and spatial expression analysis revealed that OsGLP2-1is highly expressed in leaves and panicles and sub-localized in the cell wall. Compared with empty vector transformed (control) plants, the OsGLP2-1 overexpressing plants exhibited higher levels of H2O2 both before and after pathogen inoculation. Moreover, OsGLP2-1 was significantly induced by jasmonic acid (JA). Overexpression of OsGLP2-1 induced three well-characterized defense-related genes which are associated in JA-dependent pathway after pathogen infection. Higher endogenous level of JA was also identified in OsGLP2-1 overexpressing plants than in control plants both before and after pathogen inoculation. Together, these results suggest that OsGLP2-1 functions as a positive regulator to modulate disease resistance. Its good quantitative resistance to the two major diseases in rice makes it to be a promising target in rice breeding.

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Acknowledgments

This research was supported partially by National Natural Science Foundation of China (NSFC)-International Rice Research Institute (IRRI) project (31461143019), the 973 project of Ministry of Science and Technology, China (2006BFD33320), the National Natural Science Foundation of China (30771392), the Presidential Foundation of the Guangdong Academy of Agricultural Sciences, China (201201), the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-01-24). We thank professor Jan Leach in Colorado State University, USA and Dr Hei Leung in International Rice Research Institute for their valuable discussion and suggestions on this study and during manuscript preparation. We also thank Jia Jian for technical support of sub-localization. We are grateful to Zhang Weina (Center for Agrobiological Gene Research, Guangdong Academy of Agricultural Sciences) for help with laser confocal microscopy.

Author contributions

QL conducted the quantitative qRT-PCR assay, transgenic functional confirmation and chemical treatment experiments, drafting the manuscript and proposal writing. JY and WJ evaluated the blast and Xoo resistance of the control and transgenic plants. SY conducted the quantitative analysis of the endogenous JA. SZ, JZ, TY, XW, XM, and JD participated in RNA extraction and quantitative qRT-PCR assays. XZ and BL conceived of the study, drafted proposal and corrected manuscript.

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Authors and Affiliations

  1. Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China

    Qing Liu, Shaohong Zhang, Junliang Zhao, Tifeng Yang, Xiaofei Wang, Xingxue Mao, Jingfang Dong & Bin Liu

  2. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China

    Qing Liu, Shaohong Zhang, Junliang Zhao, Tifeng Yang, Xiaofei Wang, Xingxue Mao, Jingfang Dong & Bin Liu

  3. Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China

    Jianyuan Yang, Wenjuan Wang & Xiaoyuan Zhu

  4. Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Shijuan Yan

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  1. Qing Liu
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  2. Jianyuan Yang
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Correspondence to Xiaoyuan Zhu or Bin Liu.

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Bin Liu is the first corresponding author.

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Liu, Q., Yang, J., Yan, S. et al. The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice. Plant Mol Biol 92, 411–423 (2016). https://doi.org/10.1007/s11103-016-0521-4

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