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Expression of a rice GLP in Medicago truncatula exerting pleiotropic effects on resistance against Fusarium oxysporum through enhancing FeSOD-like activity

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Abstract

To evaluate the effectiveness of a germin-like protein (GLP) in legumes against the serious soil-borne pathogen Fusarium oxysporum f. sp. lentis, an Oryza sativa root-expressed GLP (OsRGLP1) was expressed in the model legume Medicago truncatula using the recombinant vector pCOsRGLP1. The transgene was highly expressed in M. truncatula transformed lines as assessed by RT-qPCR. Consistent with the active status of the transgene there was an elevated accumulation of H2O2 in transformed progeny. Enzymatic characterization of T1 transgenic progeny showed increased superoxide dismutase (SOD) activity. The additional SOD activity in transgenic lines was insensitive to potassium cyanide and sensitive to H2O2 indicating its resemblance to FeSOD. The effectiveness of the OsRGLP1 gene was tested by monitoring the root disease after infection of wild-type and transgenic lines. Wild-type plants were greatly affected by the pathogen infection showing a percent disease index value of 50 compared to 10–18 for the transgenic lines. The tolerance of the transgenic lines leads to recovery in fresh weight and pod production to an almost normal level. Analysis of defense-related genes downstream of hydrogen peroxide (H2O2) in transgenic plants showed induction of salicylic acid and jasmonate signaling pathways and increased expression of some pathogenesis-related-1 (PR-1) genes and a plant defensin gene. Overall, the findings suggest that OsRGLP1 provides protection against the fungal pathogen F. oxysporum that may involve the direct influence of H2O2 on signaling pathways leading to the activation of defense-related genes.

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Acknowledgments

This work was financially supported by Higher Education Commission under Indigenous PhD 5000 Fellowship Program (No. 17-5-4(Ps4-279) and International Research Scholarship Initiative (No. IRSIP-21-BMS-47). The authors are grateful to Center for Application of Molecular Biology to International Agriculture, Australia for the generous gift of pCAMBIA vectors.

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Authors and Affiliations

  1. Department of Biochemistry, PMAS Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan

    Tasawar Sultana, Farah Deeba & S. M. Saqlan Naqvi

  2. Department of Plant Pathology, PMAS Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan

    Farah Naz

  3. School of Environmental and Life Sciences, The University of Newcastle, Newcastle, Australia

    Ray J. Rose

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  1. Tasawar Sultana
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  2. Farah Deeba
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  3. Farah Naz
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Correspondence to S. M. Saqlan Naqvi.

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Communicated by E. Kuzniak-Gebarowska.

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Sultana, T., Deeba, F., Naz, F. et al. Expression of a rice GLP in Medicago truncatula exerting pleiotropic effects on resistance against Fusarium oxysporum through enhancing FeSOD-like activity. Acta Physiol Plant 38, 255 (2016). https://doi.org/10.1007/s11738-016-2273-9

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