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A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)

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Abstract

In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.

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Acknowledgments

We are grateful to the Joint Center for Instruments and Research of the College of Bioresources and Agriculture at National Taiwan University for confocal microscopy and technical support. This work was supported in part by the Ministry of Science and Technology (MOST) of the Republic of China (NSC 101-2313-B-002-008-MY3 to C. Y. Hong). T. M. Wu was supported by a postdoctoral fellowship from MOST (NSC 103-2811-B-002-142).

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Author notes

  1. Tsung-Meng Wu

    Present address: Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC

  2. Yun-Yang Chao

    Present address: Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC

Authors and Affiliations

  1. Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC

    Tsung-Meng Wu, Ke-Chun Lin, Wei-Shiang Liau, Yun-Yang Chao, Ling-Hung Yang, Szu-Yun Chen & Chwan-Yang Hong

  2. Department of Life Sciences, National Central University, Jhongli City, Taoyuan County, 320, Taiwan, ROC

    Chung-An Lu

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  1. Tsung-Meng Wu
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Correspondence to Chwan-Yang Hong.

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Wu, TM., Lin, KC., Liau, WS. et al. A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.). Plant Mol Biol 90, 107–115 (2016). https://doi.org/10.1007/s11103-015-0397-8

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