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DWD HYPERSENSITIVE TO UV-B 1 is negatively involved in UV-B mediated cellular responses in Arabidopsis

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Abstract

Among T-DNA insertion mutants of various cullin4-RING ubiquitin E3 ligase (CRL4) substrate receptors, one mutant that exhibits enhanced sensitivity in response to ultraviolet-B (UV-B) illumination has been isolated and its corresponding gene has been named DWD HYPERSENSITIVE TO UV-B 1 (DHU1) in Arabidopsis. dhu1 lines showed much shorter hypocotyls than those in wild type under low doses of UV-B. Other light did not alter hypocotyl growth patterns in dhu1, indicating the hypersensitivity of dhu1 is restricted to UV-B. DHU1 was upregulated by more than two times in response to UV-B application of 1.5 μmol m−2 s−1, implying its possible involvement in UV-B signaling. DHU1 is able to bind to DDB1, an adaptor of CRL4; accordingly, DHU1 is thought to act as a substrate receptor of CRL4. Microarray data generated from wild-type and dhu1 under low doses of UV-B revealed that 209 or 124 genes were upregulated or downregulated by more than two times in dhu1 relative to wild type, respectively. About 23.4 % of the total upregulated genes in dhu1 were upregulated by more than five times in response to UV-B based on the AtGenExpress Visualization Tool data, while only about 1.4 % were downregulated to the same degree by UV-B, indicating that loss of DHU1 led to the overall enhancement of the upregulation of UV-B inducible genes. dhu1 also showed altered responsiveness under high doses of UV-B. Taken together, these findings indicate that DHU1 is a potent CRL4 substrate receptor that may function as a negative regulator of UV-B response in Arabidopsis.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2012R1A1A1001564), by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs (112030-1), and by a grant from the Next-Generation BioGreen 21 Program (No. PJ00901001), Rural Development Administration, Republic of Korea to J.-H.L. This work was also supported by grants from National Basic Research Program of China (973 Program) (2012CB910900), NIH (GM-47850) and NSF (MCB-0929100) to X.W.D.

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  1. Department of Biology Education, Pusan National University, Pusan, 609-735, Korea

    Sang-Hoon Kim, Hani Kim, Kyoung-In Seo, Soon-Hee Kim & Jae-Hoon Lee

  2. Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT, 06520-8104, USA

    Kyoung-In Seo, Xing Wang Deng & Jae-Hoon Lee

  3. University College, Yonsei University, Seoul, 120-749, Korea

    Sunglan Chung

  4. School of Life Sciences, Xiamen University, Xiamen, 361102, China

    Xi Huang

  5. Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, College of Life Sciences, Peking University, Beijing, 100871, China

    Panyu Yang & Xing Wang Deng

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Kim, SH., Kim, H., Seo, KI. et al. DWD HYPERSENSITIVE TO UV-B 1 is negatively involved in UV-B mediated cellular responses in Arabidopsis . Plant Mol Biol 86, 571–583 (2014). https://doi.org/10.1007/s11103-014-0247-0

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