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Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1

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Abstract

In comparison to herbaceous plants, studies of cold responsive genes and cold signaling in woody perennials are limited. Since most woody plants have evolved freezing tolerance (FT) in winter, together with similar lignified structures and winter adaptive mechanisms, it is more likely to find cold resistant genes in woody plants growing in temperate and arctic regions. In this study, Ammopiptanthus mongolicus, an evergreen, broadleaf and leguminous shrub was selected as a model to study cold tolerance in woody plants. Thirteen cold up-regulated cDNAs (including eight full-length cDNAs and five partial cDNAs) were cloned from A. mongolicus. One of these genes, AmEBP1, confers enhanced cold tolerance to E. coli and obvious increased freezing survival to Arabidopsis. In transgenic Arabidopsis expressing AmEBP1, transcript levels of some downstream genes in cold signaling exhibit increased accumulation upon cold treatment. Together with structural information, sub-cellular location, and promoter analysis data, it is suggested that AmEBP1 enhances plants cold tolerance by accelerating ribosome biogenesis and the concomitant translation of cold induced transcription factors and downstream protective proteins under cold stress.

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Abbreviations

AFPs:

Antifreeze proteins

CA:

Cold acclimation

cDNA-AFLP:

cDNA-amplified fragments length polymorphism

eIF2α:

α-Subunit of the eukaryotic initiation factor

FT:

Freezing tolerance

GUS:

β-Glucuronidase

HsEBP1:

An ErbB3 binding protein from Human

ICE1:

Inducer of CBF expression 1

IPTG:

Isopropyl-β-D-thiogalactopyranoside

LT:

Low temperature

NLS:

Nuclear localization signal

PKR:

dsRNA-activated protein kinase

pPKR:

Plant dsRNA-activated protein kinase

RACE:

Rapid amplification of cDNA ends

RNP:

Ribonucleoprotein

SD:

Short daylength

TAIL-PCR:

Thermal asymmetric interlaced PCR

TDF:

Transcript-derived fragment

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Acknowledgements

This research was partially supported by the 11th Five-Year Research Program of Science and Technology of China (Grant No. 2006BAD13B03) and the Science and Technology Innovation Program of Chinese Academy of Sciences (Grant No. 057310A151). We thank Prof. Weicai Yang and Dr. Dongqiao Shi for their expert technical assistance in Confocal observation.

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Authors and Affiliations

  1. The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Pengxiu Cao, Jian Song, Chunjiang Zhou, Manli Weng, Fuxin Wang, Feng Zhao & Bin Wang

  2. The Graduate School of Chinese Academy of Sciences, Beijing, 100039, China

    Pengxiu Cao

  3. Taishan Branch of Shandong Academy of Forestry Sciences, Taian, 271000, China

    Jing Liu & Dianqi Feng

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  1. Pengxiu Cao
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  2. Jian Song
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Correspondence to Dianqi Feng or Bin Wang.

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Cao, P., Song, J., Zhou, C. et al. Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1 . Plant Mol Biol 69, 529–539 (2009). https://doi.org/10.1007/s11103-008-9434-1

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