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A protein related to prokaryotic UMP kinases is involved in psaA/B transcript accumulation in Arabidopsis

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Abstract

Dpt1 (d efect in p saA/B t ranscript accumulation 1) is a novel photosystem (PS) I mutant in Arabidopsis. dpt1 mutants fail to grow photoautotrophically, and are impaired in the accumulation of psaA/B transcripts while the transcript levels for the remaining PSI subunits, for subunits of the PSII, the cyt-b 6 /f-complex, and the ribulose-1,5-bisphosphate carboxylase are comparable to the wild type. In-organello run-on transcription assays demonstrate that the lower psaA/B transcript abundance in dpt1-1 is not caused by the inability to transcribe the psaA/psaB/rps14 operon. psaA/B transcripts in the mutant are associated with polyribosomes and translated. Thus, the mutation affects post-transcriptional processes specific for psaA/B. The dpt1 gene was isolated by map-based cloning. The protein is localized in the stroma of the chloroplast and exhibits striking similarities to UMP kinases of prokaryotic origin. Our results show that the nuclear encoded protein Dpt1 is essential for retaining photosynthetic activity in higher plant chloroplasts and involved in post-transcriptional steps of psaA/B transcript accumulation. We discuss that Dpt1 may be a bifunctional protein that couples the pyrimidine metabolism to the photosynthetic electron transport.

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Abbreviations

dpt1 :

Defect in psaA/B transcript accumulation 1

PSI:

Photosystem I

UMP:

Uridine monophosphate

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Acknowledgements

This work was supported by the Friedrich-Schiller-University Jena. We thank Dr. W. Fischer for the electron micrographs, Bernard Lepetit for the help with the 77 K measurements, Dr. Masato Nakai for providing the AtCnfU-IVb antibodies, and H. Becker for skillful assistance. We thank Lars Dietzel, Sebastian Steiner and Iris Camehl for their help and the NASC stock center for providing the insertion line N829192.

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  1. Institute of General Botany and Plant Physiology, Friedrich-Schiller-Universität Jena, Dornburgerstr. 159, 07743, Jena, Germany

    Paul Hein & Ralf Oelmüller

  2. Department of Biology, Washington University, St. Louis, MO, 63130, USA

    Jana Stöckel & Stefan Bennewitz

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  1. Paul Hein
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  2. Jana Stöckel
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  3. Stefan Bennewitz
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  4. Ralf Oelmüller
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Correspondence to Ralf Oelmüller.

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Hein, P., Stöckel, J., Bennewitz, S. et al. A protein related to prokaryotic UMP kinases is involved in psaA/B transcript accumulation in Arabidopsis . Plant Mol Biol 69, 517–528 (2009). https://doi.org/10.1007/s11103-008-9433-2

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