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Characterization of KNOX genes in Medicago truncatula

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Abstract

We isolated three class I and three class II KNOX genes in Medicago truncatula. The predicted amino acid sequences suggested a possible orthology to the Arabidopsis homeodomain proteins STM, KNAT1/BP, KNAT3 and KNAT7 that was confirmed by phylogenetic and conserved structural domain analyses. Moreover, the STM-like MtKNOX1 and MtKNOX6 proteins were shown to retain the capability to interact with the Arabidopsis BELL protein partners of STM and KNAT1/BP. Amino acid residues that characterize the different classes of KNOX proteins were identified. Gene expression studies revealed organ-specificity, possible cytokinin-dependent transcriptional activation of two MtKNOXs and expression of one STM-like and a BP/KNAT1-like MtKNOX in roots. Interestingly, mRNA localization studies carried out on class I MtKNOX genes revealed important differences with previously characterised legume KNOXs. M. truncatula transcripts were not down-regulated in leaf primordia and early stages of leaf development, features shared with the more distant compound-leaved species Solanum lycopersicum.

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Acknowledgements

We thanks Josè Pio Beltran, Francisco Madueño and Ana Berbel (Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia—Spain) for kindly providing the inflorescence meristem cDNA library, for helping us to set up in situ hybridization conditions in M. truncatula and for hosting E. D. G. in their lab for a CNR short term fellowship; Giovanni Mele (CNR-IBBA, Rome, Italy) for help with two-hybrid assays; Harley M. S. Smith (Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California) for kindly providing two-hybrid constructs with Arabidopsis genes; the University of L’Aquila, in particular Prof. Laura Spanò and Prof. Anna Poma, for giving E. D. G the opportunity to attend a PhD course and critical suggestions; Renato A. Rodrigues-Pousada for a critical reading of the paper; the Samuel Roberts Noble Foundation, the Texas A&M University and the Texas Medical Center, for providing ESTs. This work was supported by the Ministero dell’Istruzione, dell’Universita` e della Ricerca (MIUR)—Fondo per gli Investimenti della Ricerca di Base (FIRB project no. RBNE018BHE) and by the Consiglio Nazionale delle Ricerche (CNR), Commessa AG.P01.003.

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  1. Institute of Biology and Agricultural Biotechnology (IBBA), Operative Unit of Rome, Consiglio Nazionale delle Ricerche (CNR), Via Salaria Km. 29,300, 00015, Monterotondo Scalo, Roma, Italy

    Elisabetta Di Giacomo, Maria Adelaide Iannelli, Giulio Testone, Domenico Mariotti & Giovanna Frugis

  2. Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo de Lellis snc, Viterbo, 01100, Italy

    Francesco Sestili

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  1. Elisabetta Di Giacomo
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  2. Francesco Sestili
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  3. Maria Adelaide Iannelli
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  6. Giovanna Frugis
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Correspondence to Giovanna Frugis.

Additional information

GenBank accession numbers for MtKNOX1 were EF128056 and AB033478.1 for nucleotide and amino acid sequence, respectively; for MtKNOX2 were EF128057 and AB033479.1; for MtKNOX3 were EF128058 and AB033480.1; for MtKNOX4 were EF128059 and AB033481.1; for MtKNOX5 were EF128060 and AB033482.1; for MtKNOX6 were EF128061 and AB033483.1.

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Di Giacomo, E., Sestili, F., Iannelli, M.A. et al. Characterization of KNOX genes in Medicago truncatula . Plant Mol Biol 67, 135–150 (2008). https://doi.org/10.1007/s11103-008-9307-7

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