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The expression of iron homeostasis-related genes during rice germination

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Abstract

To characterize Fe homeostasis during the early stages of seed germination, a microarray analysis was performed. mRNAs extracted from fully mature seeds or seeds harvested 1–3 days after sowing were hybridized to a rice microarray containing approximately 22,000 cDNA oligo probes. Many Fe deficiency-inducible genes were strongly expressed throughout early seed germination. These results suggest that the demand for Fe is extremely high during germination.

Under Fe-deficient conditions, rice produces and secretes a metal-cation chelator called deoxymugineic acid (DMA) to acquire Fe from the soil. In addition, DMA and its intermediate nicotianamine (NA) are thought to be involved in long distance Fe transport in rice. Using promoter-β-glucuronidase (GUS) analysis, we investigated the expression patterns during seed germination of the Fe deficiency-inducible genes OsNAS1, OsNAS2, OsNAS3, OsNAAT1, and OsDMAS1, which encode enzymes that participate in the biosynthesis of DMA, and the transporter genes OsYSL2 and OsIRT1, which are involved in Fe transport. All of these genes were expressed in germinating seeds prior to protrusion of the radicle. These results suggest that DMA and NA are produced and involved in Fe transport during germination.

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Acknowledgements

We thank Dr Takanori Kobayashi for reading manuscript and helpful discussions, and Dr Yoshiaki Nagamura of the Rice Genome Project and the NIAS DNA Bank (National Institute of Agrobiological Sciences, Tsukuba, Japan) for support with the 22K oligo array analysis.

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Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Tomoko Nozoye, Michiko Takahashi, Yasuhiro Ishimaru, Hiromi Nakanishi, Satoshi Mori & Naoko K. Nishizawa

  2. CREST, Tokyo, Japan

    Haruhiko Inoue & Naoko K. Nishizawa

  3. Laboratory of Plant Biotechnology, Department of Global Agricultural Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan

    Naoko K. Nishizawa

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  1. Tomoko Nozoye
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  2. Haruhiko Inoue
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  3. Michiko Takahashi
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  4. Yasuhiro Ishimaru
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  5. Hiromi Nakanishi
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Correspondence to Naoko K. Nishizawa.

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Nozoye, T., Inoue, H., Takahashi, M. et al. The expression of iron homeostasis-related genes during rice germination. Plant Mol Biol 64, 35–47 (2007). https://doi.org/10.1007/s11103-007-9132-4

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