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Modification of Endogenous Natural Genes by Gene Targeting in Rice and Other Higher Plants

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Abstract

The capability to modify a genomic sequence into a designed sequence is a powerful tool for biologists and breeders to elucidate the function of an individual gene and its cis-acting elements of multigene families in the genome. Gene targeting refers to the alteration of a specific DNA sequence in an endogenous gene at its original locus in the genome. In higher plants, however, the overwhelming occurrence of the random integration of transgenes by non-homologous end-joining is the main obstacle to develop efficient gene targeting. Two approaches have been undertaken to modify a genomic sequence in higher plants– chimeric RNA/DNA oligonucleotide-directed gene targeting to generate a site-specific base conversion, and homologous recombination-dependent gene targeting to produce either a base change or a gene replacement in a sequence-specific manner. The successful and reproducible targeting of an endogenous gene by homologous recombination, independently of gene-specific selection by employing a strong positive-negative selection, has been demonstrated for the first time in rice, an important staple food and a model plant for other cereal species. This review addresses the current status of targeting of an endogenous natural gene in rice and other higher plants and discusses possible models for Agrobacterium- mediated gene targeting by homologous recombination using a strong positive–negative selection.

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References

  • A. Aguilera (2002) ArticleTitleThe connection between transcription and genomic instability EMBO J. 21 195–201 Occurrence Handle10.1093/emboj/21.3.195 Occurrence Handle11823412

    Article  PubMed  Google Scholar 

  • M.S. Andersen C.B. Sorensen L. Bolund T.G. Jensen (2002) ArticleTitleMechanisms underlying targeted gene correction using chimeric RNA/DNA and single-stranded DNA oligonucleotides J. Mol. Med. 80 770–781 Occurrence Handle10.1007/s00109-002-0393-8 Occurrence Handle12483462

    Article  PubMed  Google Scholar 

  • N. Bechtold J. Ellis G. Pelletier (1993) ArticleTitleIn planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants C. R. Acad. Sci. Paris Life Sci. 316 1194–1199

    Google Scholar 

  • P.R. Beetham P.B. Kipp X.L. Sawycky C.J. Arntzen G.D. May (1999) ArticleTitleA tool for functional plant genomics: chimeric RNA/DNA oligonucleotides cause in vivo gene-specific mutations Proc. Natl. Acad. Sci. USA 96 8774–8778 Occurrence Handle10.1073/pnas.96.15.8774 Occurrence Handle10411951

    Article  PubMed  Google Scholar 

  • J.W. Bodley V.G. Johnson B.A. Wilson S.R. Blanke J.R. Murphy A.M. Pappenheimer SuffixJr. R.J. Collier S.L. Lessnick C. Bruce R.L. Baldwin M.P. Chang L.T. Nakanura B.J. Wisnieski (1990) ArticleTitleDoes diphtheria toxin have nuclease activity Science 250 832–838 Occurrence Handle2237435

    PubMed  Google Scholar 

  • A.B. Britt G.D. May (2003) ArticleTitleRe-engineering plant gene targeting Trends Plant Sci. 8 90–95 Occurrence Handle10.1016/S1360-1385(03)00002-5 Occurrence Handle12597876

    Article  PubMed  Google Scholar 

  • V. Brunaud S. Balzergue B. Dubreucq S. Aubourg F. Samson S. Chauvin N. Bechtold C. Cruaud R. Rose ParticleDe G. Pelletier L. Lepiniec M. Caboche A. Lecharny (2002) ArticleTitleT-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites EMBO Rep. 3 1152–1157 Occurrence Handle10.1093/embo-reports/kvf237 Occurrence Handle12446565

    Article  PubMed  Google Scholar 

  • M.P. Chang R.L. Baldwin C. Bruce B.J. Wisnieski (1989) ArticleTitleSecond cytotoxic pathway of diphtheria toxin suggested by nuclease activity Science 246 1165–1168 Occurrence Handle2531465

    PubMed  Google Scholar 

  • M.-D.M. Chilton Q. Que (2003) ArticleTitleTargeted integration of T-DNA into the tobacco genome at double-stranded breaks: new insights on the mechanism of T-DNA integration Plant Physiol. 133 956–965 Occurrence Handle10.1104/pp.103.026104 Occurrence Handle14551336

    Article  PubMed  Google Scholar 

  • M. Czako G. An (1991) ArticleTitleExpression of DNA coding for diphtheria toxin chain A is toxic to plant cells Plant Physiol. 95 687–692

    Google Scholar 

  • C.D. Day V.F. Irish (1997) ArticleTitleCell ablation and the analysis of plant development Trends Plant Sci. 2 106–111 Occurrence Handle10.1016/S1360-1385(97)01004-2

    Article  Google Scholar 

  • M. Delseny (2003) ArticleTitleTowards an accurate sequence of the rice genome Curr. Opin. Plant Biol. 6 101–105 Occurrence Handle10.1016/S1369-5266(03)00010-4 Occurrence Handle12667864

    Article  PubMed  Google Scholar 

  • L.Y. Fang P.R. Gross C.-H. Chen M. Lillis (1992) Sequence of two acetohydroxyacid synthase genes from Zea mays Plant Mol. Biol. 18–1185

    Google Scholar 

  • Q. Feng Y. Zhang P. Hao S. Wang G. Fu Y. Huang Y. Li J. Zhu Y. Liu X. Hu P. Jia Y. Zhang Q. Zhao K. Ying S. Yu Y. Tang Q. Weng L. Zhang Y. Lu J. Mu Y. Lu L.S. Zhang Z. Yu D. Fan X. Liu T. Lu C. Li Y. Wu T. Sun H. Lei T. Li H. Hu J. Guan M. Wu R. Zhang B. Zhou Z. Chen L. Chen Z. Jin R. Wang H. Yin Z. Cai S. Ren G. Lv W. Gu G. Zhu Y. Tu J. Jia Y. Zhang J. Chen H. Kang X. Chen C. Shao Y. Sun Q. Hu X. Zhang W. Zhang L. Wang C. Ding H. Sheng J. Gu S. Chen L. Ni F. Zhu W. Chen L. Lan Y. Lai Z. Cheng M. Gu J. Jiang J. Li G. Hong Y. Xue B. Han (2002) ArticleTitleSequence and analysis of rice chromosome 4 Nature 420 316–320 Occurrence Handle10.1038/nature01183 Occurrence Handle12447439

    Article  PubMed  Google Scholar 

  • M.E. Gallego P. Sirand-Pugnet C.I. White (1999) ArticleTitlePositive–negative selection and T-DNA stability in Arabidopsis transformation Plant Mol. Biol. 39 83–93 Occurrence Handle10.1023/A:1006192225464 Occurrence Handle10080711

    Article  PubMed  Google Scholar 

  • H.B. Gamper A. Cole-Strauss R. Metz H. Parekh R. Kumar E.B. Kmiec (2000) ArticleTitleA plausible mechanism for gene correction by chimeric oligonucleotides Biochemistry 39 5808–5816 Occurrence Handle10.1021/bi9921891 Occurrence Handle10801331

    Article  PubMed  Google Scholar 

  • S.B. Gelvin (2000) ArticleTitleAgrobacterium and plant genes involved in T-DNA transfer and integration Annu. Rev. Plant Physiol. Plant Mol. Biol. 51 223–256 Occurrence Handle10.1146/annurev.arplant.51.1.223 Occurrence Handle15012192

    Article  PubMed  Google Scholar 

  • S.B. Gelvin (2003) ArticleTitleAgrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool Microbiol. Mol. Biol. Rev. 67 16–37 Occurrence Handle10.1128/MMBR.67.1.16-37.2003 Occurrence Handle12626681

    Article  PubMed  Google Scholar 

  • S.A. Goff D. Ricke T.-H. Lan G. Presting R. Wang M. Dunn J. Glazebrook A. Sessions P. Oeller H. Varma D. Hadley D. Hutchison C. Martin F. Katagiri B.M. Lange T. Moughamer Y. Xia P. Budworth J. Zhong T. Miguel U. Paszkowski S. Zhang M. Colbert W.-L. Sun L. Chen B. Cooper S. Park T.C. Wood L. Mao P. Quail R. Wing R. Dean Y. Yu A. Zharkikh R. Shen S. Sahasrabudhe A. Thomas R. Cannings A. Gutin D. Pruss J. Reid S. Tavtigian J. Mitchell G. Eldredge T. Scholl R.M. Miller S. Bhatnagar N. Adey T. Rubano N. Tusneem R. Robinson J. Feldhaus T. Macalma A. Oliphant S. Briggs (2002) ArticleTitleA draft sequence of the rice genome (Oryza sativa L ssp. japonica). Science 296 92–100 Occurrence Handle11935018

    PubMed  Google Scholar 

  • M. Gong Y.S. Rong (2003) ArticleTitleTargeting multi-cellular organisms Curr. Opin. Genet. Dev. 13 215–220 Occurrence Handle10.1016/S0959-437X(03)00018-2 Occurrence Handle12672500

    Article  PubMed  Google Scholar 

  • V. Gorbunova A.A. Levy (1999) ArticleTitleHow plants make ends meet: DNA double-strand break repair Trends Plant Sci. 4 263–269 Occurrence Handle10.1016/S1360-1385(99)01430-2 Occurrence Handle10407442

    Article  PubMed  Google Scholar 

  • M. Hanin J. Paszkowski (2003) ArticleTitlePlant genome modification by homologous recombination Curr. Opin. Plant Biol. 6 157–162 Occurrence Handle10.1016/S1369-5266(03)00016-5 Occurrence Handle12667873

    Article  PubMed  Google Scholar 

  • M. Hanin S. Volrath A. Bogucki M. Briker E. Ward J. Paszkowski (2001) ArticleTitleGene targeting in Arabidopsis Plant J. 28 671–677 Occurrence Handle10.1046/j.1365-313x.2001.01183.x Occurrence Handle11851913

    Article  PubMed  Google Scholar 

  • R. Hayama S. Yokoi S. Tamaki M. Yano K. Shimamoto (2003) ArticleTitleAdaptation of photoperiodic control pathways produces short-day flowering in rice Nature 422 719–722 Occurrence Handle10.1038/nature01549 Occurrence Handle12700762

    Article  PubMed  Google Scholar 

  • Y. Hiei T. Komari T. Kubo (1997) ArticleTitleTransformation of rice mediated by Agrobacterium tumefaciens Plant Mol. Biol. 35 205–218 Occurrence Handle10.1023/A:1005847615493 Occurrence Handle9291974

    Article  PubMed  Google Scholar 

  • Y. Hiei S. Ohta T. Komari T. Kumashiro (1994) ArticleTitleEfficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA Plant J. 6 271–282 Occurrence Handle10.1046/j.1365-313X.1994.6020271.x Occurrence Handle7920717

    Article  PubMed  Google Scholar 

  • H. Hirochika E. Guiderdoni G. An Y.-I. Hsing M.Y. Eun C.-D Han N. Upadhyaya S. Ramachandran Q. Zhang A. Pereira V. Sundaresan H. Leung (2004) ArticleTitleRice mutant resources for gene discovery Plant Mol. Biol. 54 325–334 Occurrence Handle10.1023/B:PLAN.0000036368.74758.66 Occurrence Handle15284490

    Article  PubMed  Google Scholar 

  • B. Hohn H. Puchta (1999) ArticleTitleGene therapy in plants Proc. Natl. Acad. Sci. USA 96 8321–8323 Occurrence Handle10.1073/pnas.96.15.8321 Occurrence Handle10411868

    Article  PubMed  Google Scholar 

  • B. Hohn H. Puchta (2003) ArticleTitleSome like it sticky: targeting of the rice gene Waxy Trends Plant Sci. 8 51–53 Occurrence Handle10.1016/S1360-1385(03)00004-9 Occurrence Handle12597868

    Article  PubMed  Google Scholar 

  • S. Iida R. Terada (2004) ArticleTitleA tale of two integrations, transgene and T-DNA: gene targeting by homologous recombination in rice Curr. Opin. Biotechnol. 15 132–138 Occurrence Handle10.1016/j.copbio.2004.02.005 Occurrence Handle15081051

    Article  PubMed  Google Scholar 

  • R.A. Jefferson T.A. Kavanagh M.W. Bevan (1987) ArticleTitleGUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants EMBO J. 6 3901–3907 Occurrence Handle3327686

    PubMed  Google Scholar 

  • S.A. Kempin S.J. Liljegren L.M. Block S.D. Rounsley M.F. Yanofsky E. Lam (1997) ArticleTitleTargeted disruption in Arabidopsis Nature 389 802–803 Occurrence Handle10.1038/39770 Occurrence Handle9349810

    Article  PubMed  Google Scholar 

  • A. Kochevenko L. Willmitzer (2003) ArticleTitleChimeric RNA/DNA oligonucleotide-based site-specific modification of the tobacco acetolactate syntase gene Plant Physiol. 132 174–184 Occurrence Handle10.1104/pp.102.016857 Occurrence Handle12746523

    Article  PubMed  Google Scholar 

  • A. Kohli R.M. Twyman R. Abranches E. Wegel E. Stoger P. Christou (2003) ArticleTitleTransgene integration, organization and interaction in plants Plant Mol. Biol. 52 247–258 Occurrence Handle10.1023/A:1023941407376 Occurrence Handle12856933

    Article  PubMed  Google Scholar 

  • A.M. Koltunow J. Truettner K.H. Cox M. Wallroth R.B. Goldberg (1990) ArticleTitleDifferent temporal and spatial gene expression patterns occur during anther development Plant Cell 2 1201–1224 Occurrence Handle10.1105/tpc.2.12.1201 Occurrence Handle12354953

    Article  PubMed  Google Scholar 

  • S. Kumar M. Fladung (2001) ArticleTitleControlling transgene integration in plants Trends Plant Sci. 6 155–159 Occurrence Handle10.1016/S1360-1385(01)01890-8 Occurrence Handle11286920

    Article  PubMed  Google Scholar 

  • M. Kusaba (2004) ArticleTitleRNA interference in crop plants Curr. Opin. Biotechnol. 15 139–143 Occurrence Handle10.1016/j.copbio.2004.02.004 Occurrence Handle15081052

    Article  PubMed  Google Scholar 

  • K.Y. Lee P. Lund K. Lowe P. Dunsmuir (1990) ArticleTitleHomologous recombination in plant cells after Agrobacterium-mediated transformation Plant Cell 2 415–425 Occurrence Handle10.1105/tpc.2.5.415 Occurrence Handle2152167

    Article  PubMed  Google Scholar 

  • K.Y. Lee J. Townsend J. Tepperman M. Black C.F. Chui B. Mazur P. Dunsmuir J. Bedbrook (1988) ArticleTitleThe molecular basis of sulfonylurea herbicide resistance in tobacco EMBO J. 7 1241–1248

    Google Scholar 

  • S.J. Liljegren M.F. Yanofsky (1998) ArticleTitle…response: Targeting Arabidopsis Trends Plant Sci. 3 79–80

    Google Scholar 

  • S.L. Mansour K.R. Thomas M.R. Capecchi (1988) ArticleTitleDisruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes Nature 336 348–352 Occurrence Handle10.1038/336348a0 Occurrence Handle3194019

    Article  PubMed  Google Scholar 

  • T. Mengiste J. Paszkowski (1999) ArticleTitleProspects for the precise engineering of plant genomes by homologous recombination Biol. Chem. 380 749–758 Occurrence Handle10.1515/BC.1999.095 Occurrence Handle10494824

    Article  PubMed  Google Scholar 

  • Z.-H. Miao E. Lam (1995) ArticleTitleTargeted disruption of the TGA3 locus in Arabidopsis thaliana Plant J. 7 359–365 Occurrence Handle10.1046/j.1365-313X.1995.7020359.x Occurrence Handle7704051

    Article  PubMed  Google Scholar 

  • D. Miki K. Shimamoto (2004) ArticleTitleSimple RNAi vectors for stable and transient suppression of gene function in rice Plant Cell Physiol. 45 490–495 Occurrence Handle10.1093/pcp/pch048 Occurrence Handle15111724

    Article  PubMed  Google Scholar 

  • R. Morton P.J.J. Hooykaas (1995) ArticleTitleGene replacement Mol. Breed. 1 123–132 Occurrence Handle10.1007/BF01249697

    Article  Google Scholar 

  • S.B. Narasimhulu X.-B. Deng R. Sarria S.B. Gelvin (1996) ArticleTitleEarly transcription of Agrobactertium T-DNA genes in tobacco and maize Plant Cell 8 873–886 Occurrence Handle10.1105/tpc.8.5.873 Occurrence Handle8672885

    Article  PubMed  Google Scholar 

  • O. Nilsson E. Wu D.S. Wolfe D. Weigel (1998) ArticleTitleGenetic ablation of flowers in transgenic Arabidopsis Plant J. 15 799–804 Occurrence Handle10.1046/j.1365-313X.1998.00260.x Occurrence Handle9807818

    Article  PubMed  Google Scholar 

  • T.J. Oh G.D. May (2001) ArticleTitleOligonucleotide-directed plant gene targeting Curr. Opin. Biotechnol. 12 169–172 Occurrence Handle10.1016/S0958-1669(00)00194-4 Occurrence Handle11287232

    Article  PubMed  Google Scholar 

  • A. Okuzaki K. Toriyama (2004) ArticleTitleChimeric RNA/DNA oligonucleotide-directed gene targeting in rice Plant Cell Rep. 22 509–512 Occurrence Handle10.1007/s00299-003-0698-2 Occurrence Handle14634786

    Article  PubMed  Google Scholar 

  • A.M. Pappenheimer ParticleJr. (1977) ArticleTitleDiphtheria toxin Annu. Rev. Biochem. 46 69–94 Occurrence Handle10.1146/annurev.bi.46.070177.000441 Occurrence Handle20040

    Article  PubMed  Google Scholar 

  • F. Paques J.E. Haber (1999) ArticleTitleMultiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev. 63 349–404 Occurrence Handle10357855

    PubMed  Google Scholar 

  • J. Paszkowski M. Baur A. Bogucki I. Potrykus (1988) ArticleTitleGene targeting in plants EMBO J. 7 4021–4026

    Google Scholar 

  • R.J. Perera C.G. Linard E.R. Signer (1993) ArticleTitleCytosine deaminase as a negative selective marker for Arabidopsis Plant Mol. Biol. 23 793–799 Occurrence Handle10.1007/BF00021534 Occurrence Handle8251632

    Article  PubMed  Google Scholar 

  • F. Prado A. Aguilera (2003) ArticleTitleControl of cross-over by single-strand DNA resection Trends Genet. 19 428–431 Occurrence Handle10.1016/S0168-9525(03)00173-2 Occurrence Handle12902160

    Article  PubMed  Google Scholar 

  • H. Puchta (1998) ArticleTitleRepair of genomic double-strand breaks in somatic plant cells by one-sided invasion of homologous sequences Plant J. 13 331–339 Occurrence Handle10.1046/j.1365-313X.1998.00035.x

    Article  Google Scholar 

  • H. Puchta (2002) ArticleTitleGene replacement by homologous recombination in plants Plant Mol. Biol. 48 173–182 Occurrence Handle10.1023/A:1013761821763 Occurrence Handle11860208

    Article  PubMed  Google Scholar 

  • A. Ray M. Langer (2002) ArticleTitleHomologous recombination: ends as the means Trends Plant Sci. 7 435–440 Occurrence Handle10.1016/S1360-1385(02)02327-0 Occurrence Handle12399177

    Article  PubMed  Google Scholar 

  • B. Reiss (2003) ArticleTitleHomologous recombination and gene targeting in plant cells Int. Rev. Cytol. 228 85–139 Occurrence Handle14667043

    PubMed  Google Scholar 

  • M.C. Rice G.D. May P.B. Kipp H. Parekh E.B. Kmiec (2000) ArticleTitleGenetic repair of mutations in plant cell-free extracts directed by specific chimeric oligonucleotides Plant Physiol. 123 427–437 Occurrence Handle10.1104/pp.123.2.427 Occurrence Handle10859173

    Article  PubMed  Google Scholar 

  • E. Risseeuw R. Offringa M.E.I. Franke-Dijk Particlevan P.J.J. Hooykaas (1995) ArticleTitleTargeted recombination in plants using Agrobacterium coincides with additional rearrangements at the target locus Plant J. 7 109–119 Occurrence Handle10.1046/j.1365-313X.1995.07010109.x Occurrence Handle7894502

    Article  PubMed  Google Scholar 

  • C. Sallaud D. Meynard J. van Boxtel C. Gay M. Bes J.P. Brizard P. Larmande D. Ortega M. Raynal M. Portefaix P.B.F. Ouwerkerk S. Rueb M. Delseny E. Guiderdoni (2003) ArticleTitleHighly efficient production and characterization of T-DNA plants for rice (Oryza sativa L.) functional genomics Theor. Appl. Genet. 106 1396–1408 Occurrence Handle12677401

    PubMed  Google Scholar 

  • T. Sasaki T. Matsumoto K. Yamamoto K. Sakata T. Baba Y. Katayose J. Wu Y. Niimura Z. Cheng Y. Nagamura B.A. Antonio H. Kanamori S. Hosokawa M. Masukawa K. Arikawa Y. Chiden M. Hayashi M. Okamoto T. Ando H. Aoki K. Arita M. Hamada C. Harada S. Hijishita M. Honda Y. Ichikawa A. Idonuma M. Iijima M. Ikeda M. Ikeno S. Ito T. Ito Y. Ito Y. Ito A. Iwabuchi K. Kamiya W. Karasawa S. Katagiri A. Kikuta N. Kobayashi I. Kono K. Machita T. Maehara H. Mizuno T. Mizubayashi Y. Mukai H. Nagasaki M. Nakashima Y. Nakama Y. Nakamichi M. Nakamura N. Namiki M. Negishi I. Ohta N. Ono S. Saji K. Sakai M. Shibata T. Shimokawa A. Shomura J. Song Y. Takazaki K. Terasawa K. Tsuji K. Waki H. Yamagata H. Yamane S. Yoshiki R. Yoshihara K. Yukawa H. Zhong H. Iwama T. Endo H. Ito J.H. Hahn H.-I. Kim M.-Y. Eun M. Yano J. Jiang T. Gojobori (2002) ArticleTitleThe genome sequence and structure of rice chromosome 1 Nature 420 312–316 Occurrence Handle10.1038/nature01184 Occurrence Handle12447438

    Article  PubMed  Google Scholar 

  • K. Sathasivan G.W. Haughn N. Murai (1991) ArticleTitleMolecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var Columbia Plant Physiol. 97 1044–1050

    Google Scholar 

  • J. Stougaard (1993) ArticleTitleSubstrate-dependent negative selection in plants using a bacterial cytosine deaminase gene Plant J. 3 755–761 Occurrence Handle10.1111/j.1365-313X.1993.00755.x

    Article  Google Scholar 

  • R. Tarchini P. Biddle R. Wineland S. Tingey A. Rafalski (2000) ArticleTitleThe complete sequence of 340 kb DNA around the rice Adh1–Adh2 region reveals interrupted colinearity with maize chromosome 4 Plant Cell 12 381–391 Occurrence Handle10.1105/tpc.12.3.381 Occurrence Handle10715324

    Article  PubMed  Google Scholar 

  • R. Terada H. Asao S. Iida (2004) ArticleTitleA large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection for gene targeting in rice (Oryza sativa L.) Plant Cell Rep. 22 653–659 Occurrence Handle10.1007/s00299-003-0752-0 Occurrence Handle14740168

    Article  PubMed  Google Scholar 

  • R. Terada H. Urawa Y. Inagaki K. Tsugane S. Iida (2002) ArticleTitleEfficient gene targeting by homologous recombination in rice Nat. Biotechnol. 20 1030–1034 Occurrence Handle10.1038/nbt737 Occurrence Handle12219079

    Article  PubMed  Google Scholar 

  • The rice chromosome 10 sequencing consortium (2003) ArticleTitleIn-depth view of structure, activity, and evolution of rice chromosome 10 Science 300 1566–1569 Occurrence Handle10.1126/science.1083523 Occurrence Handle12791992

    Article  PubMed  Google Scholar 

  • M.K. Thorsness M.K. Kandasamy M.E. Nasrallah J.B. Nasrallah (1991) ArticleTitleA Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana Dev. Biol. 143 173–184 Occurrence Handle10.1016/0012-1606(91)90064-A Occurrence Handle1985017

    Article  PubMed  Google Scholar 

  • T. Thykjaer J. Finnemann L. Schauser L. Christensen C. Poulsen J. Stougaard (1997) ArticleTitleGene targeting approaches using positive–negative selection and large flanking regions Plant Mol. Biol. 35 523–530 Occurrence Handle10.1023/A:1005865600319 Occurrence Handle9349274

    Article  PubMed  Google Scholar 

  • B. Tinland (1996) ArticleTitleThe integration of T-DNA into plant genomes Trends Plant Sci. 1 178–184 Occurrence Handle10.1016/1360-1385(96)10020-0

    Article  Google Scholar 

  • T. Tzfira L.R. Frankman M. Vaidya V. Citovsky (2003) ArticleTitleSite-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates Plant Physiol. 133 1011–1023 Occurrence Handle10.1104/pp.103.032128 Occurrence Handle14551323

    Article  PubMed  Google Scholar 

  • T. Tzfira Y. Rhee M.-H. Chen T. Kunik V. Citovsky (2000) ArticleTitleNucleic acid transport in plant-microbe interactions: the molecules that walk through the walls Annu. Rev. Microbiol. 54 187–219 Occurrence Handle10.1146/annurev.micro.54.1.187 Occurrence Handle11018128

    Article  PubMed  Google Scholar 

  • D. Valvekens M. Montagu ParticleVan M. Lijsebettens ParticleVan (1988) ArticleTitleAgrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection Proc. Natl. Acad. Sci. USA 85 5536–5540

    Google Scholar 

  • A.C. Vergunst P.J.J. Hooykaas (1999) ArticleTitleRecombination in the plant genome and its application in biotechnology Crit. Rev. Plant Sci. 18 1–31

    Google Scholar 

  • H.X. Wang J.-F. Viret A. Eldridge R. Perera E.R. Signer M. Chiurazzi (2001) ArticleTitlePositive–Negative selection for homologous recombination in Arabidopsis Gene 272 249–255 Occurrence Handle10.1016/S0378-1119(01)00532-7 Occurrence Handle11470531

    Article  PubMed  Google Scholar 

  • M. Yamaizumi E. Mekada T. Uchida Y. Okada (1978) ArticleTitleOne molecule of diphtheria toxin fragment A introduced into a cell can kill the cell Cell 15 245–250 Occurrence Handle10.1016/0092-8674(78)90099-5 Occurrence Handle699044

    Article  PubMed  Google Scholar 

  • J. Yu S. Hu J. Wang G.K.-S. Wong S. Li B. Liu Y. Deng L. Dai Y. Zhou X. Zhang M. Cao J. Liu J. Sun J. Tang Y. Chen X. Huang W. Lin C. Ye W. Tong L. Cong J. Geng Y. Han L. Li W. Li G. Hu X. Huang W. Li J. Li Z. Liu L. Li J. Liu Q. Qi J. Liu L. Li T. Li X. Wang H. Lu T. Wu M. Zhu P. Ni H. Han W. Dong X. Ren X. Feng P. Cui X. Li H. Wang X. Xu W. Zhai Z. Xu J. Zhang S. He J. Zhang J. Xu K. Zhang X. Zheng J. Dong W. Zeng L. Tao J. Ye J. Tan X. Ren X. Chen J. He D. Liu W. Tian C. Tian H. Xia Q. Bao G. Li H. Gao T. Cao J. Wang W. Zhao P. Li W. Chen X. Wang Y. Zhang J. Hu J. Wang S. Liu J. Yang G. Zhang Y. Xiong Z. Li L. Mao C. Zhou Z. Zhu R. Chen B. Hao W. Zheng S. Chen W. Guo G. Li S. Liu M. Tao J. Wang L. Zhu L. Yuan H. Yang (2002) ArticleTitleA draft sequence of the rice genome (Oryza sativa L ssp. indica). Science 296 79–92 Occurrence Handle11935017

    PubMed  Google Scholar 

  • T. Zhu K. Mettenburg D.J. Peterson L. Tagliani C.L. Baszczynski (2000) ArticleTitleEngineering herbicide-resistant maize using chimeric RNA/DNA oligonucleotides Nature Biotechnol. 18 555–558 Occurrence Handle10.1038/75435

    Article  Google Scholar 

  • T. Zhu D.J. Peterson L. Tagliani G. St. Clair C.L. Baszczynski B. Bowen (1999) ArticleTitleTargeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides Proc. Natl. Acad. Sci. USA 96 8768–8773 Occurrence Handle10.1073/pnas.96.15.8768 Occurrence Handle10411950

    Article  PubMed  Google Scholar 

  • J. Zupan T.R. Muth O. Draper P. Zambryski (2000) ArticleTitleThe transfer of DNA from Agrobacterium tumefaciences into plants: a feast of fundamental insights Plant J. 23 11–28 Occurrence Handle10.1046/j.1365-313x.2000.00808.x Occurrence Handle10929098

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Molecular Genetics, National Institutes of Natural Sciences, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, 444-8585, Japan

    Shigeru Iida

  2. Department of Molecular Biomechanics, The Graduate University for Advanced Studies, 444-8585, Japan

    Rie Terada

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  2. Rie Terada
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Correspondence to Shigeru Iida.

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Iida, S., Terada, R. Modification of Endogenous Natural Genes by Gene Targeting in Rice and Other Higher Plants. Plant Mol Biol 59, 205–219 (2005). https://doi.org/10.1007/s11103-005-2162-x

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