Skip to main content
SpringerLink
Log in

Dual DNA Binding Property of ABA insensitive 3 Like Factors Targeted to Promoters Responsive to ABA and Auxin

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The ABA responsive ABI3 and the auxin responsive ARF family of transcription factors bind the CATGCATG (Sph) and TGTCTC core motifs in ABA and auxin response elements (ABRE and AuxRE), respectively. Several evidences indicate ABI3s to act downstream to auxin too. Because DNA binding domain of ABI3s shows significant overlap with ARFs we enquired whether auxin responsiveness through ABI3s could be mediated by their binding to canonical AuxREs. Investigations were undertaken through in␣vitro gel mobility shift assays (GMSA) using the DNA binding domain B3 of PvAlf (Phaseolus vulgaris ABI3 like factor) and upstream regions of auxin responsive gene GH3 (−267 to −141) and ABA responsive gene Em (−316 to −146) harboring AuxRE and ABRE, respectively. We demonstrate that B3 domain of PvAlf could bind AuxRE only when B3 was associated with its flanking domain B2 (B2B3). Such strict requirement of B2 domain was not observed with ABRE, where B3 could bind with or without being associated with B2. This dual specificity in DNA binding of ABI3s was also demonstrated with nuclear extracts of cultured cells of Arachis hypogea. Supershift analysis of ABRE and AuxRE bound nuclear proteins with antibodies raised against B2B3 domains of PvAlf revealed that ABI3 associated complexes were detectable in association with both cis elements. Competition GMSA confirmed the same complexes to bind ABRE and AuxRE. This dual specificity of ABI3 like factors in DNA binding targeted to natural promoters responsive to ABA and auxin suggests them to have a potential role in conferring crosstalk between these two phytohormones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ABA:

abscisic acid

ABI3:

ABA insensitive 3

BAP:

benzylamino purine

DBD:

DNA binding domain

FUS3:

FUSCA3

NAA:

1-naphthaline acetic acid

PvAlf:

Phaseolus vulgaris ABI3 like factor

RAV1:

related to ABI3/VP1

VP1:

viviparous 1

References

  • A. Basu M. Chawla-Sarkar S. Chakrabarti S.K. DasGupta (2002) ArticleTitleOrigin binding activity of the mycobacterial plasmid pAL5000 replication protein RepB is stimulated through interactions with host factors and coupled expression of repA J. Bact. 184 2204–2214 Occurrence Handle10.1128/JB.184.8.2204-2214.2002 Occurrence Handle11914352

    Article  PubMed  Google Scholar 

  • A.J. Bobb M.S. Chern M.M. Bustos (1997) ArticleTitleConserved RY-repeats mediate transactivation of seed-specific promoters by the developmental regulator PvAlf Nucleic Acids Res. 1 641–647 Occurrence Handle10.1093/nar/25.3.641

    Article  Google Scholar 

  • A.J. Bobb H.G. Eiben M.M. Bustos (1995) ArticleTitlePvAlf, an embryo-specific acidic transcriptional activator enhances gene expression from phaseolin and phytohemagglutinin promoters Plant J. 8 331–343 Occurrence Handle10.1046/j.1365-313X.1995.08030331.x Occurrence Handle7550372

    Article  PubMed  Google Scholar 

  • M.M. Bradford (1976) Anal. Biochem. 72 248–254 Occurrence Handle942051

    PubMed  Google Scholar 

  • S.M. Brady S.F. Sarkar D. Bonetta P. McCourt (2003) ArticleTitleThe abscicic acid insensitive 3(ABI3) gene is modulated by farnesylation and is involved in auxin signaling and lateral root development in Arabidopsis Plant J. 34 67–75 Occurrence Handle10.1046/j.1365-313X.2003.01707.x Occurrence Handle12662310

    Article  PubMed  Google Scholar 

  • R. Carranco1 M.B. Chandrasekharan C.T. James T.C. Hall (2004) ArticleTitleInteraction of PvAlf and VP1 B3 domains with the b-phaseolin promoter Plant Mol. Biol. 55 221–237 Occurrence Handle10.1007/s11103-004-0512-8 Occurrence Handle15604677

    Article  PubMed  Google Scholar 

  • C.B. Carson T. Hattori L. Rosenkrans V. Vasil I.K. Vasil P.A. Peterson D.R. McCarty (1997) ArticleTitleThe quiescent/colorless alleles of viviparous1 show that the conserved B3 domain of VP1 is not essential for ABA-regulated gene expression in the seed Plant J. 12 1231–1240 Occurrence Handle10.1046/j.1365-313x.1997.12061231.x Occurrence Handle9450338

    Article  PubMed  Google Scholar 

  • K. Dehesh H. Hung J.M. Tepperman P.H. Quail (1992) ArticleTitleGT-2: a transcription factor with twin autonomous DNA-binding domains of closely related but different target sequence specificity EMBO J. 11 4131–4144 Occurrence Handle1396594

    PubMed  Google Scholar 

  • I. Smet ParticleDe L. Signora T. Beeckman D. Inze C.H. Foyer H. Zhang (2003) ArticleTitleAn abscisic acid-sensitive checkpoint in lateral root development of Arabidopsis Plant J. 33 543–555 Occurrence Handle10.1046/j.1365-313X.2003.01652.x Occurrence Handle12581312

    Article  PubMed  Google Scholar 

  • I. Ezcurra P. Wycliffe L. Nehlin M. Ellerstrom L. Rask (2000) ArticleTitleTransactivation of the Brassica napus napin promoter by ABI3 requires interaction of the conserved B2 and B3 domains of ABI3 with different cis-elements: B2 mediates activation though an ABRE, whereas B3 interacts with an RY/G-box Plant J. 24 57–66 Occurrence Handle10.1046/j.1365-313x.2000.00857.x Occurrence Handle11029704

    Article  PubMed  Google Scholar 

  • S. Gazzarrini P. McCourt (2003) ArticleTitleCross-talk in plant hormone signalling: what Arabidopsis mutants are telling us Ann. Bot. (London) 91 605–612 Occurrence Handle10.1093/aob/mcg064

    Article  Google Scholar 

  • J. Giraudat B.M. Hauge C. Valon J. Smalle F. Parcy H.M. Goodman (1992) ArticleTitleIsolation of the Arabidopsis ABI3 gene by positional cloning Plant Cell 4 1251–1261 Occurrence Handle10.1105/tpc.4.10.1251 Occurrence Handle1359917

    Article  PubMed  Google Scholar 

  • T.J. Guilfoyle T. Ulmasov G. Hagen (1998) ArticleTitleThe ARF family of transcription factors and their role in plant hormone-responsive transcription Cell Mol. Life Sci. 54 619–627 Occurrence Handle10.1007/s000180050190 Occurrence Handle9711229

    Article  PubMed  Google Scholar 

  • E. Harlow D. Lane (1988) Antibodies: a laboratory manual Cold Spring Harbor Laboratory Cold Spring Harbor, NY

    Google Scholar 

  • T. Hattori T. Terada S. Hamasuna (1995) ArticleTitleRegulation of the Osem gene by abscisic acid and the transcriptional activator VP1: analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1 Plant J. 7 913–925 Occurrence Handle10.1046/j.1365-313X.1995.07060913.x Occurrence Handle7599651

    Article  PubMed  Google Scholar 

  • T. Hattori V. Vasil L. Rosenkrans L.C. Hannah D.R. McCarty I.K. Vasil (1992) ArticleTitleThe Viviparous-1 gene and abscisic acid activate the C1 regulatory gene for anthocyanin biosynthesis during seed maturation in maize Genes Dev. 6 609–618 Occurrence Handle1532784

    PubMed  Google Scholar 

  • A. Hill A. Nantel C.D. Rock R.S. Quatrano (1996) ArticleTitleA conserved domain of the viviparous-1 gene product enhances the DNA binding activity of the bZIP protein EmBP-1 and other transcription factors J. Biol. Chem. 271 3366–3374 Occurrence Handle10.1074/jbc.271.49.31290 Occurrence Handle8631935

    Article  PubMed  Google Scholar 

  • T. Hobo Y. Kowyama T. Hattori (1999) ArticleTitleA bZIP factor TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription Proc. Natl. Acad. Sci. USA 21 15348–15353 Occurrence Handle10.1073/pnas.96.26.15348

    Article  Google Scholar 

  • U. Hoecker I.K. Vasil D.R. McCarty (1995) ArticleTitleIntegrated control of seed maturation and germination programs by activator and repressor functions of Viviparous-1 of maize Genes Dev. 15 2459–2469

    Google Scholar 

  • Y. Kagaya K. Ohmiya T. Hattori (1999) ArticleTitleRAV1, a novel DNA-binding protein, binds to bipartite recognition sequence though two distinct DNA-binding domains uniquely found in higher plants Nucleic Acids Res. 15 470–478 Occurrence Handle10.1093/nar/27.2.470

    Article  Google Scholar 

  • H. Kende J. Zeevaart (1997) ArticleTitleThe five “classical” plant hormones Plant Cell 9 1197–1210 Occurrence Handle10.1105/tpc.9.7.1197 Occurrence Handle12237383

    Article  PubMed  Google Scholar 

  • G. Li K.J. Bishop M.B. Chandrasekharan T.C. Hall (1999) ArticleTitleβ-Phaseolin gene activation is a two-step process: PvAlf-facilitated chromatin modification followed by abscisic acid-mediated gene activation Proc. Natl. Acad. Sci. USA 96 7104–7109 Occurrence Handle10.1073/pnas.96.12.7104 Occurrence Handle10359846

    Article  PubMed  Google Scholar 

  • Z.B. Liu T. Ulmasov X. Shi G. Hagen T.J. Guilfoyle (1994) ArticleTitleThe soybean GH3 promoter contains multiple auxin-inducible elements Plant Cell 6 645–657 Occurrence Handle10.1105/tpc.6.5.645 Occurrence Handle8038604

    Article  PubMed  Google Scholar 

  • H. Luerssen V. Kirik P. Herrmann S. Misera (1998) ArticleTitleFUSCA3 encodes a protein with a conserved VP1/AB13-like B3 domain which is of functional importance for the regulation of seed maturation in Arabidopsis thaliana Plant J. 15 755–764 Occurrence Handle10.1046/j.1365-313X.1998.00259.x Occurrence Handle9807814

    Article  PubMed  Google Scholar 

  • W.R. Marcotte SuffixJr. C.C. Bayley R.S. Quatrano (1988) ArticleTitleRegulation of a wheat promoter by abscisic acid in rice protoplasts Nature 335 454–457 Occurrence Handle10.1038/335454a0

    Article  Google Scholar 

  • D.R. McCarty T. Hattori C.B. Carson V. Vasil M. Lazar I.K. Vasil (1991) ArticleTitleThe Viviparous-1 developmental gene of maize encodes a novel transcriptional activator Cell 66 895–905 Occurrence Handle10.1016/0092-8674(91)90436-3 Occurrence Handle1889090

    Article  PubMed  Google Scholar 

  • S.G. Moller N.-H. Chua (1999) ArticleTitleInteractions and intersections of plant signaling pathways J. Mol. Biol. 293 219–234 Occurrence Handle10.1006/jmbi.1999.2992 Occurrence Handle10550205

    Article  PubMed  Google Scholar 

  • Nag, R., Maity, M.K., Seal, A., Hazra, A. and DasGupta, M. 2005. Protein turnover in response to transient exposure to exogenous auxin is necessary for restoring auxin autotrophy in a stressed Arachis hypogea cell culture. Plant cell tissue and organ culture (in Press)

  • E. Nambara K. Keith P. McCourt S. Naito (1995) ArticleTitleA regulatory role for the ABI3 gene in the establishment of embryo maturation in Arabidopsis thaliana Development 121 629–636

    Google Scholar 

  • F. Parcy C. Valon M. Raynal P. Gaubier-Comella M. Delseny J. Giraudat (1994) ArticleTitleRegulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid Plant Cell 6 1567–1582 Occurrence Handle10.1105/tpc.6.11.1567 Occurrence Handle7827492

    Article  PubMed  Google Scholar 

  • W. Reidt T. Wohlfarth M. Ellerstrom A. Czihal A. Tewes I. Ezcurra L. Rask H. Baumlein (2000) ArticleTitleGene regulation during late embryogenesis: the RY motif of maturation-specific gene promoters is a direct target of the FUS3 gene product Plant J. 21 401–408 Occurrence Handle10.1046/j.1365-313x.2000.00686.x Occurrence Handle10758492

    Article  PubMed  Google Scholar 

  • A. Rohde W. Ardiles-Diaz M. Montagu ParticleVan W. Boerjan (1998) ArticleTitleIsolation and expression analysis of an ABSCISIC ACID-INSENSITIVE 3 (ABI3) homologue from Populus trichocarpa J. Exp. Bot. 49 1059–1060 Occurrence Handle10.1093/jexbot/49.323.1059

    Article  Google Scholar 

  • A. Rohde R. Rycke ParticleDe T. Beeckman G. Engler M. Montagu ParticleVan W. Boerjan (2000) ArticleTitleABI3 affects plastid differentiation in dark-grown Arabidopsis seedlings Plant Cell 12 35–52 Occurrence Handle10.1105/tpc.12.1.35 Occurrence Handle10634906

    Article  PubMed  Google Scholar 

  • A. Rohde C. Grunau L. Beck ParticleDe M. Montagu ParticleVan A. Rosenthal W. Boerjan (1999) ArticleTitleCarpel, a new Arabidopsis epi-mutant of the SUPERMAN gene: phenotypic analysis and DNA methylation status Plant Cell Physiol. 40 961–972 Occurrence Handle10588067

    PubMed  Google Scholar 

  • A. Rohde E. Prinsen R. Rycke ParticleDe G. Engler M. Montagu ParticleVan W. Boerjan (2002) ArticleTitlePtABI3 impinges on the growth and differentiation of embryonic leaves during bud set in poplar Plant Cell 14 1885–1890 Occurrence Handle10.1105/tpc.003186 Occurrence Handle12172029

    Article  PubMed  Google Scholar 

  • A. Seal A. Hazra R. Nag S. Chaudhuri M. DasGupta (2001) ArticleTitleExogenous auxin depletion renders an Arachis hypogea cell suspension culture sensitive to water loss without affecting cell growth Plant Cell Rep. 20 567–573 Occurrence Handle10.1007/s002990100359

    Article  Google Scholar 

  • R. Solano C. Nieto J. Avila L. Canas I. Diaz J. Paz-Ares (1995) ArticleTitleDual DNA binding specificity of a petal epidermis-specific MYB transcription factor (MYB.Ph3) from Petunia hybrida EMBO J. 14 1773–1784 Occurrence Handle7737128

    PubMed  Google Scholar 

  • J.K. Strauss L.J. Maher SuffixIII (1994) ArticleTitleDNA bending by asymmetric phosphate neutralization Science 266 IssueID5192 1829–1834 Occurrence Handle7997878

    PubMed  Google Scholar 

  • M. Suzuki M.G. Ketterling Q.B. Li D.R. McCarty (2003) ArticleTitleViviparous1 alters global gene expression patterns through regulation of abscisic signaling Plant Physiol. 132 1664–1677 Occurrence Handle10.1104/pp.103.022475 Occurrence Handle12857845

    Article  PubMed  Google Scholar 

  • M. Suzuki C.Y. Kao S. Cocciolone D.R. McCarty (2001) ArticleTitleMaize VP1 complements Arabidopsis abi3 and confers a novel ABA/auxin interaction in roots Plant J. 28 409–418 Occurrence Handle10.1046/j.1365-313X.2001.01165.x Occurrence Handle11737778

    Article  PubMed  Google Scholar 

  • M. Suzuki C.Y. Kao D.R. McCarty (1997) ArticleTitleThe conserved B3 domain of VIVIPAROUS1 has a cooperative DNA binding activity Plant Cell 9 799–807 Occurrence Handle10.1105/tpc.9.5.799 Occurrence Handle9165754

    Article  PubMed  Google Scholar 

  • J.D. Thompson D.G. Higgins T.J Gibson (1994) Nucleic Acids Res 22 4673–4680 Occurrence Handle7984417

    PubMed  Google Scholar 

  • T. Ulmasov G. Hagen T.J. Guilfoyle (1997) ArticleTitleARF1, a transcription factor that binds to auxin response elements Science 20 1865–1868 Occurrence Handle10.1126/science.276.5320.1865

    Article  Google Scholar 

  • T. Ulmasov G. Hagen T.J. Guilfoyle (1999) ArticleTitleDimerization and DNA binding of auxin response factors Plant J. 19 309–319 Occurrence Handle10.1046/j.1365-313X.1999.00538.x Occurrence Handle10476078

    Article  PubMed  Google Scholar 

  • T. Ulmasov Z.-B. Liu G. Hagen T.J. Guilfoyle (1995) ArticleTitleComposite structure of auxin response elements Plant Cell 7 1611–1623 Occurrence Handle10.1105/tpc.7.10.1611 Occurrence Handle7580254

    Article  PubMed  Google Scholar 

  • K. Yamasaki T. Kigawa M. Inoue M. Tateno T. Yamasaki T. Yabuki M. Aoki E. Seki T. Matsuda Y. Tomo N. Hayami T. Terada M. Shirouzu T. Osanai A. Tanaka M. Seki K. Shinozaki S. Yokoyama (2004) ArticleTitleSolution structure of the B3 DNA binding domain of the Arabidopsis Cold-Responsive Transcription Factor RAV1 Plant Cell 16 3448–3459 Occurrence Handle10.1105/tpc.104.026112 Occurrence Handle15548737

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Calcutta University, 35, Ballygunge Circular Road, 700019, Calcutta

    Ronita Nag, Manas Kanti Maity & Maitrayee DasGupta

Authors
  1. Ronita Nag
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manas Kanti Maity
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maitrayee DasGupta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maitrayee DasGupta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nag, R., Maity, M.K. & DasGupta, M. Dual DNA Binding Property of ABA insensitive 3 Like Factors Targeted to Promoters Responsive to ABA and Auxin. Plant Mol Biol 59, 821–838 (2005). https://doi.org/10.1007/s11103-005-1387-z

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11103-005-1387-z

Keywords

Search

Navigation