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Photosystem 2 photochemistry and pigment composition of a yellow mutant of rice (Oryza sativa L.) under different irradiances

  • Original Papers
  • Published:
Photosynthetica

Abstract

A yellow leaf colouration mutant (named ycm) generated from rice T-DNA insertion lines was identified with less grana lamellae and low thylakoid membrane protein contents. At weak irradiance [50 µmol(photon) m−2 s−1], chlorophyll (Chl) contents of ycm were ≈20 % of those of WT and Chl a/b ratios were 3-fold that of wild type (WT). The leaf of ycm showed lower values in the actual photosystem 2 (PS2) efficiency (ΦPS2), photochemical quenching (qP), and the efficiency of excitation capture by open PS2 centres 1 (Fv′/Fm′) than those of WT, except no difference in the maximal efficiency of PS2 photochemistry (Fv/Fm). With progress in irradiance [100 and 200 µmol(photon) m−2 s−1], there was a change in the photosynthetic pigment stoichiometry. In ycm, the increase of total Chl contents and the decrease in Chl a/b ratio were observed. ΦPS2, qP, and Fv′/Fm′ of ycm increased gradually along with the increase of irradiance but still much less than in WT. The increase of xanthophyll ratio [(Z+A)/(V+A+Z)] associated with non-photochemical quenching (qN) was found in ycm which suggested that ycm dissipated excess energy through the turnover of xanthophylls. No significant differences in pigment composition were observed in WT under various irradiances, except Chl a/b ratio that gradually decreased. Hence the ycm mutant developed much more tardily than WT, which was caused by low photon energy utilization independent of irradiance.

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Abbreviations

A:

antheraxanthin

Chl:

chlorophyll

F0 :

minimal fluorescence in dark-adapted leaves

Fm :

maximal fluorescence in dark-adapted leaves

Fv :

maximum variable fluorescence in dark-adapted leaves

F0′:

minimal fluorescence in light-adapted leaves

Fm′:

maximal fluorescence in light-adapted leaves

Fv′:

maximum variable fluorescence in light-adapted leaves

Fv/Fm :

maximal efficiency of PS2 photochemistry

Fv′/Fm′:

efficiency of excitation energy capture by open PS2 reaction centres

PS:

photosystem

qN :

non-photochemical quenching

qP :

photochemical quenching

V:

violaxanthin

Z:

zeaxanthin

ΦPS2 :

actual PS2 efficiency

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Authors and Affiliations

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Q. Chen, L. F. Wang, N. Su, J. L. Wang, H. Q. Zhai & J. M. Wan

  2. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China

    H. D. Qin, H. B. Niu & J. M. Wan

Authors
  1. Q. Chen
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  2. L. F. Wang
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  3. N. Su
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  4. H. D. Qin
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  5. H. B. Niu
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  6. J. L. Wang
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  7. H. Q. Zhai
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  8. J. M. Wan
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Correspondence to J. M. Wan.

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Chen, Q., Wang, L.F., Su, N. et al. Photosystem 2 photochemistry and pigment composition of a yellow mutant of rice (Oryza sativa L.) under different irradiances. Photosynthetica 46, 35–39 (2008). https://doi.org/10.1007/s11099-008-0007-y

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  • DOI: https://doi.org/10.1007/s11099-008-0007-y

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