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Antimicrobial Drugs Encapsulated in Fibrin Nanoparticles for Treating Microbial Infested Wounds

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A Correction to this article was published on 19 April 2019

ABSTRACT

Purpose

In vitro evaluation of antibacterial and antifungal drugs encapsulated fibrin nanoparticles to prove their potential prospect of using these nanocomponent for effective treatment of microbial infested wounds.

Methods

Surfactant-free oil-in-water emulsification-diffusion method was adopted to encapsulate 1 mg/ml each of antimicrobial drugs (Ciprofloxacin and Fluconazole) in 4 ml of aqueous fibrinogen suspension and subsequent thrombin mediated cross linking to synthesize drug loaded fibrin nanoparticles.

Results

Ciprofloxacin loaded fibrin nanoparticles (CFNPs) showed size range of 253 ± 6 nm whereas that of Fluconazole loaded fibrin nanoparticles (FFNPs) was 260 ± 10 nm. Physico chemical characterizations revealed the firm integration of antimicrobial drugs within fibrin nanoparticles. Drug release studies performed at physiological pH 7.4 showed a release of 16% ciprofloxacin and 8% of fluconazole while as the release of ciprofloxacin at alkaline pH 8.5, was 48% and that of fluconazole was 37%. The antimicrobial activity evaluations of both drug loaded systems independently showed good antibacterial activity against Escherichia coli (E.coli), Staphylococcus aureus (S. aureus) and antifungal activity against Candida albicans (C. albicans). The in vitro toxicity of the prepared drug loaded nanoparticles were further analyzed using Human dermal fibroblast cells (HDF) and showed adequate cell viability.

Conclusion

The efficacies of both CFNPs and FFNPs for sustained delivery of encapsulated anti microbial drugs were evaluated in vitro suggesting its potential use for treating microbial infested wounds (diabetic foot ulcer).

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ACKNOWLEDGMENTS AND DISCLOSURES

B. Maria Alphonsa, P. T. Sudheesh Kumar and G. Praveen contributed equally. The authors are grateful to Department of Biotechnology (DBT), India, for the financial support under a grant (BT/PR6758/NNT/28/620/2012 dated 14-08-2013). Raja Biswas acknowledges Ramalingaswami Fellowship, Department of Biotechnology, India, for the financial support. P T Sudheesh Kumar and G. Praveen acknowledge the Council of Scientific and Industrial Research, India for the Senior Research Fellowship. We are also grateful to Mr. Sajin P. Ravi for his help in SEM analysis. We are grateful to Amrita Centre for Nanosciences and Molecular Medicine for the infrastructure support.

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Authors and Affiliations

  1. Amrita Centre for Nanosciences and Molecular Medicine Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, 682041, India

    B. Maria Alphonsa, P. T. Sudheesh Kumar, G. Praveen, Raja Biswas, K. P. Chennazhi & R. Jayakumar

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  1. B. Maria Alphonsa
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  2. P. T. Sudheesh Kumar
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  3. G. Praveen
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  4. Raja Biswas
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  5. K. P. Chennazhi
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  6. R. Jayakumar
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Correspondence to K. P. Chennazhi or R. Jayakumar.

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Alphonsa, B.M., Sudheesh Kumar, P.T., Praveen, G. et al. Antimicrobial Drugs Encapsulated in Fibrin Nanoparticles for Treating Microbial Infested Wounds. Pharm Res 31, 1338–1351 (2014). https://doi.org/10.1007/s11095-013-1254-6

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  • DOI: https://doi.org/10.1007/s11095-013-1254-6

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