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Protein-Silicone Oil Interactions: Comparative Effect of Nonionic Surfactants on the Interfacial Behavior of a Fusion Protein

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Abstract

Purpose

To study the effect of three nonionic surfactants on the protein-silicone oil interactions.

Methods

The adsorption of Tween® 80, Pluronic® F68 and Tween® 20 at the silicone oil/water interface (using shifts in frequency (ΔF) and resistance (ΔR) with quartz crystal microbalance) was compared to the adsorption at air/water interface (using surface tension). Effect of surfactants on protein adsorption to the silicone oil/water interface was studied in sequential- and co-adsorption modes. Protein-surfactant binding in the bulk was measured using dynamic surface tension method.

Results

Saturation of air/water and silicone oil/water interfaces by surfactants was observed at similar bulk concentrations. ΔF due to protein adsorption to the interface decreased only when surfactant was present as a pre-adsorbed species. Insignificant differences in the dynamic surface tension values of surfactant solutions were observed in the presence of protein.

Conclusions

Similar hydrophobic forces were responsible for driving the surfactant adsorption at both air/water and silicone oil/water interfaces. Surfactants were effective in reducing the protein adsorption to the silicone oil only when introduced before or along with the protein. No significant binding between the protein and surfactants was observed in the bulk.

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Acknowledgments and Disclosures

Biogen Idec is gratefully acknowledged for the material and financial support of this work. Financial support to N.D. in the form of Gerald J. Jackson memorial award and University of Connecticut graduate school doctoral dissertation fellowship are also acknowledged.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, 69 N Eagleville Road, Unit 3092, Storrs, Connecticut, 06269, USA

    Nitin Dixit & Devendra S. Kalonia

  2. Protein Pharmaceutical Development, Biogen Idec, 14 Cambridge Center, Cambridge, Massachusetts, 02142, USA

    Kevin M. Maloney

Authors
  1. Nitin Dixit
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  2. Kevin M. Maloney
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  3. Devendra S. Kalonia
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Correspondence to Devendra S. Kalonia.

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Dixit, N., Maloney, K.M. & Kalonia, D.S. Protein-Silicone Oil Interactions: Comparative Effect of Nonionic Surfactants on the Interfacial Behavior of a Fusion Protein. Pharm Res 30, 1848–1859 (2013). https://doi.org/10.1007/s11095-013-1028-1

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  • DOI: https://doi.org/10.1007/s11095-013-1028-1

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