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Opsonization, Biodistribution, Cellular Uptake and Apoptosis Study of PEGylated PBCA Nanoparticle as Potential Drug Delivery Carrier

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An Erratum to this article was published on 13 August 2011

ABSTRACT

Purpose

For nanocarrier-based targeted delivery systems, preventing phagocytosis for prolong circulation half life is a crucial task. PEGylated poly(n-butylcyano acrylate) (PBCA) NP has proven a promising approach for drug delivery, but an easy and reliable method of PEGylation of PBCA has faced a major bottleneck.

Methods

PEGylated PBCA NPs containing docetaxel (DTX) by modified anionic polymerization reaction in aqueous acidic media containing amine functional PEG were made as an single step PEGylation method. In vitro colloidal stability studies using salt aggregation method and antiopsonization property of prepared NPs using mouse macrophage cell line RAW264 were performed. In vitro performance of anticancer activity of prepared formulations was checked on MCF7 cell line. NPs were radiolabeled with 99mTc and intravenously administered to study blood clearance and biodistribution in mice model.

Results

These formulations very effectively prevented phagocytosis and found excellent carrier for drug delivery purpose. In vivo studies display long circulation half life of PBCA-PEG20 NP in comparison to other formulations tested.

Conclusions

The PEGylated PBCA formulation can work as a novel tool for drug delivery which can prevent RES uptake and prolong circulation half life.

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Abbreviations

DTX:

docetaxel

EPR:

enhanced permeation and retention

NP:

nanoparticle

PBCA:

poly(n-butylcyano acrylate)

PEG:

polyethylene glycol

PMN:

polymorphonuclear cells

RES:

reticuloendothelial system

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ACKNOWLEDGMENTS & DISCLOSURES

This research was supported by National Doctoral Fellowship by All India Council of Technical Education (AICTE) (1-10/RID/NDF-PG/(39)2008-2009), India. The authors are also thankful to Tong Shen Enterprise, Taiwan, for providing a free gift sample of n-butyl cyanoacrylate monomer.

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Authors and Affiliations

  1. Pharmacy Department TIFAC Centre of Relevance & Excellence in New Drug Delivery Systems G.H. Patel Pharmacy Building, The Maharaja Sayajirao University of Baroda, Donor’s Plaza, Fatehgunj, Vadodara, 390002, India

    Kiran Ramanlal Chaudhari, Mukesh Ukawala, Arehalli S. Manjappa, Abhinesh Kumar & Piyush Kishor Mundada

  2. School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland

    Kiran Ramanlal Chaudhari & Jukka Mönkkönen

  3. Department of Radiopharmaceuticals and Radiation Biology, Institute of Nuclear Medicine and Allied Sciences (INMAS), Brigadier S. K. Mazumdar Road, Delhi, 110054, India

    Kiran Ramanlal Chaudhari, Anil Kumar Mishra & Rashi Mathur

  4. ISF College of Pharmacy, Firozpur G. T. Road, Moga, 142001, Punjab, India

    Rayasa S. Ramchandra Murthy

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  1. Kiran Ramanlal Chaudhari
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Correspondence to Rayasa S. Ramchandra Murthy.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s11095-011-0565-8

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Figure S-1

Fourier transform spectroscopy (FTIR) reports of PBCA-PEG, PBCA and PEG polymer. (JPEG 50 kb)

High resolution image (TIFF 1404 kb)

Figure S-2

H1 Nuclear magnetic resonance (NMR) reports of PBCA-PEG polymer of PBCA-PEG20 NP. (JPEG 29 kb)

High resolution image (TIFF 4229 kb)

Figure S-3

Gel Permeation Chromatography (GPC) reports of PBCA NP and PBCA-PEG20 NP. (JPEG 37 kb)

High resolution image (TIFF 5239 kb)

Table SI

% Release of 6-coumarin from PBCA NP and PEGylated PBCA NP using PBS (pH 7.4) with 0.5% tween after 6 h. (DOCX 10 kb)

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Chaudhari, K.R., Ukawala, M., Manjappa, A.S. et al. Opsonization, Biodistribution, Cellular Uptake and Apoptosis Study of PEGylated PBCA Nanoparticle as Potential Drug Delivery Carrier. Pharm Res 29, 53–68 (2012). https://doi.org/10.1007/s11095-011-0510-x

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  • DOI: https://doi.org/10.1007/s11095-011-0510-x

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