The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250 × 4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 μm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile at a ratio of 80:20 (v/v), column temperature 30°C; absorption spectra were recorded over the range 190 – 400 nm. This chromatography system can be used for preliminary identification of flavonoids in plant materials.
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V. L. Shelyuto (Deceased).
Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 45, No. 1, pp. 35 – 38, January, 2011.
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Moiseev, D.V., Buzuk, G.N. & Shelyuto, V.L. Identification of flavonoids in plants by HPLC. Pharm Chem J 45, 47–50 (2011). https://doi.org/10.1007/s11094-011-0558-2
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DOI: https://doi.org/10.1007/s11094-011-0558-2