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Flavonoids by HPLC

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Natural Products

Abstract

Flavonoids are secondary plant metabolites that are synthesized via the shikimate pathway. HPLC has been an important tool for the separation of these metabolites in the last 4 decades. The coupling of HPLC with a number of detection technologies either online, in tandem, or off-line enables the identification of flavonoids in plant, food, and biological samples. This chapter provides an overview of flavonoid analysis by HPLC, including extraction of flavonoids in both aglycones and glycosides, separation by a selection of stationary and mobile phases, and finally, detection and identification by UV–VIS, fluorescence, electrochemical, mass spectrometry, and NMR spectroscopy.

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Authors and Affiliations

  1. Institute for Health Research and Policy, London Metropolitan University, 166-220 Holloway Road, London, UK

    Prof. S. W. Annie Bligh & Olumuyiwa Ogegbo

  2. The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Traditional Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China

    Zheng-Tao Wang

Authors
  1. Prof. S. W. Annie Bligh
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  2. Olumuyiwa Ogegbo
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  3. Zheng-Tao Wang
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Correspondence to S. W. Annie Bligh .

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Editors and Affiliations

  1. Botany Department, M.L. Sukhadia University, Durga Nursery Road, Udaipur, 313001, Rajasthan, India

    Kishan Gopal Ramawat

  2. Institute of Vine and Wine Sciences, Biological-Active Plant Substances Study, University of Bordeaux, 210 Chemin de Leysotte CS 50008, Villenave d'Ornon, 33882, France

    Jean-Michel Mérillon

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Bligh, S.W.A., Ogegbo, O., Wang, ZT. (2013). Flavonoids by HPLC. In: Ramawat, K., Mérillon, JM. (eds) Natural Products. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6_97

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