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Evaluation of Resveratrol and Piceatannol Anticonvulsant Potential in Adult Zebrafish (Danio rerio)

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Abstract

Epilepsy is a common neurological disorder which affects 50 million people worldwide. Patients with epilepsy may present cognitive deficits and psychological impairment. Currently, 30% of patients fail to respond to any available antiseizure drug, and a significant number of patients do not well tolerate the offered treatments. Then, it is necessary to find out alternatives for controlling epileptic seizures. Studies have shown that despite its neuroprotective effects, resveratrol shows poor anticonvulsant properties. Resveratrol analog, piceatannol, possesses higher biological activity than resveratrol and could be an alternative to control seizure. Thus, the present study investigated the effects of resveratrol and piceatannol in pentylenetetrazole-induced seizures in adult zebrafish (Danio rerio). Only the experimental positive control (diazepam) showed anticonvulsant effect in this study. In addition, no behavioral changes were observed 24 h after seizure occurrence. Finally, the expression of genes related to neuronal activity (c-fos), neurogenesis (p70S6Ka and p70S6Kb), inflammatory response (interleukin 1β), and cell apoptosis (caspase-3) did not change by pentylenetetrazole-induced seizures. Therefore, we failed to observe any anticonvulsant and neuroprotective potential of resveratrol and piceatannol in adult zebrafish. However, resveratrol and piceatannol benefits in epilepsy are not discharged, and more studies are necessary.

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Data Availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC 15/2021, Grant Number 2021TR001226), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (PIBIC/CNPq; PDE/CNPq #203013/2019-0), Governo do Estado de Santa Catarina (Programa UNIEDU), and Universidade Comunitária da Região de Chapecó (Unochapecó).

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Authors and Affiliations

  1. Curso de Ciências Biológicas, Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brazil

    Jefferson Pedroso, Sabrina Ester Schneider & Anna Maria Siebel

  2. División Ornitología, Museo Argentino de Ciencias Naturales, Buenos Aires, Argentina

    Cássia Alves Lima-Rezende

  3. Programa de Pós-Graduação em Ciências Ambientais, Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brazil

    Cássia Alves Lima-Rezende, Gean Pablo S. Aguiar, Liz Girardi Müller, J. Vladimir Oliveira & Anna Maria Siebel

  4. Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil

    J. Vladimir Oliveira

  5. Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Angelo Piato

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  1. Jefferson Pedroso
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Contributions

Jefferson Pedroso, Sabrina Ester Schneider, Cássia Alves Lima-Rezende, Gean Pablo S. Aguiar, Liz Girardi Müller, J. Vladimir Oliveira, Angelo Piato and Anna Maria Siebel designed and conducted experiments, and analyzed data. Liz Girardi Müller, J. Vladimir Oliveira, Angelo Piato and Anna Maria Siebel wrote the main manuscript text.

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Correspondence to Anna Maria Siebel.

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Pedroso, J., Schneider, S.E., Lima-Rezende, C.A. et al. Evaluation of Resveratrol and Piceatannol Anticonvulsant Potential in Adult Zebrafish (Danio rerio). Neurochem Res 47, 3250–3260 (2022). https://doi.org/10.1007/s11064-022-03656-3

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