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Exogenous Adipokine Peptide Resistin Protects Against Focal Cerebral Ischemia/Reperfusion Injury in Mice

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An Erratum to this article was published on 25 July 2017

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Abstract

Previous studies have demonstrated that plasma resistin levels were increased in patients with acute ischemic stroke. However, the role of resistin after ischemic brain injury is still unclear. In this study, we investigated the protective effects of resistin on cerebral ischemia/reperfusion injury in a middle cerebral artery occlusion mouse model. We found that resistin (i.c.v.) significantly reduced infarct volume and improved neurological deficits after 45 min of ischemia and 24 h of reperfusion. Furthermore, our data demonstrate that intraperitoneal administration of resistin (10 µg/kg body weight) also had protective effects on infarct volume, indicating the crossing of resistin through the impaired BBB after ischemia injury. Resistin treatment reduced cleaved protein level of Poly(ADP-ribose)polymerase-1 (PARP-1), a marker of cellular apoptosis, showing the anti-apoptotic activity of resistin. Resistin increased the level of phosphorylated Akt after ischemic brain injury. The neuroprotective effect of resistin was partially reversed by a PI3K inhibitor wortmannin, demonstrating that the PI3K/Akt signal pathway is involved in the anti-apoptotic mechanisms of resistin. Finally, we found that resistin treatment improved neurological function recovery at 14 days after treatment, including balance ability and muscle strength. Given these findings, resistin may have therapeutic potential for the treatment of stroke.

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  • 25 July 2017

    An erratum to this article has been published.

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Acknowledgements

This study was supported by the grants from the National Natural Science Foundation of China (81601154) and the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

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Authors and Affiliations

  1. Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou City, China

    Jiangtao Zhu, Di Wu, Chenyu Zhao, Man Luo & Xingshun Xu

  2. Institute of Neuroscience, Soochow University, Suzhou City, Jiangsu, China

    Xingshun Xu & Zhigang Miao

  3. Cecile Cox Quillen Laboratory of Geriatrics, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA

    Ronald C. Hamdy & Balvin H. L. Chua

Authors
  1. Jiangtao Zhu
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  2. Di Wu
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  3. Chenyu Zhao
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  4. Man Luo
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  5. Ronald C. Hamdy
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  6. Balvin H. L. Chua
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  7. Xingshun Xu
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  8. Zhigang Miao
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Correspondence to Zhigang Miao.

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The authors declare that they have no conflict of interest.

Additional information

Jiangtao Zhu and Di Wu are co-first authors.

An erratum to this article is available at https://doi.org/10.1007/s11064-017-2359-9.

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Zhu, J., Wu, D., Zhao, C. et al. Exogenous Adipokine Peptide Resistin Protects Against Focal Cerebral Ischemia/Reperfusion Injury in Mice. Neurochem Res 42, 2949–2957 (2017). https://doi.org/10.1007/s11064-017-2326-5

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  • DOI: https://doi.org/10.1007/s11064-017-2326-5

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