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P2Y13 Receptor-Mediated Rapid Increase in Intracellular Calcium Induced by ADP in Cultured Dorsal Spinal Cord Microglia

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Abstract

P2Y receptors have been implicated in the calcium mobilization by the response to neuroexcitatory substances in neurons and astrocytes, but little is known about P2Y receptors in microglia cells. In the present study, the effects of ADP on the intracellular calcium concentration ([Ca2+]i) in cultured dorsal spinal cord microglia were detected with confocal laser scanning microscopy using fluo-4/AM as a calcium fluorescence indicator that could monitor real-time alterations of [Ca2+]i. Here we show that ADP (0.01–100 μM) causes a rapid increase in [Ca2+]i with a dose-dependent manner in cultured microglia. The action of ADP on [Ca2+]i was significantly blocked by MRS2211 (a selective P2Y13 receptor antagonist), but was unaffected by MRS2179 (a selective P2Y1 receptor antagonist) or MRS2395 (a selective P2Y12 receptor antagonist), which suggest that P2Y13 receptor may be responsible for ADP-evoked Ca2+ mobilization in cultured microglia. P2Y13-evoked Ca2+ response can be obviously inhibited by BAPTA-AM and U-73122, respectively. Moreover, removal of extracellular Ca2+ (by EGTA) also can obvious suppress the Ca2+ mobilization. These results means both intracellular calcium and extracellular calcium are potentially important mechanisms in P2Y13 receptor-evoked Ca2+ mobilization. However, P2Y13 receptor-evoked Ca2+ response was not impaired after CdCl2 and verapamil administration, which suggest that voltage-operated Ca2+ channels may be not related with P2Y13-evoked Ca2+ response. In addition, Ca2+ mobilization induced by ADP was abolished by different store-operated Ca2+ channels (SOCs) blocker, 2-APB (50 μM) and SKF-96365 (1 mM), respectively. These observations suggest that the activation of P2Y13 receptor might be involved in the effect of ADP on [Ca2+]i in cultured dorsal spinal cord microglia. Furthermore, our results raise a possibility that P2Y13 receptor activation causes Ca2+ release from Ca2+ store, which leads to the opening of SOCs.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (No. 31000497 and 31460266), Key Project of Chinese Ministry of Education (212154), Program for New Century Excellent Talents in University (NCET-13-1070), Scientific Research Foundation for Excellent Talents in Guizhou Province (2012–93). We are very grateful to the other staff of Department of Physiology. We also thank Wang Huan and Guo-Hui Bai (Central Laboratory, Zunyi Medical College) for their technique assistance in laser scanning confocal microscopy.

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  1. Department of Physiology, Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical College, 201 Dalian Street, Zunyi, 563000, Guizhou Province, People’s Republic of China

    Junwei Zeng, Gaoxia Wang, Xiaohong Liu, Chunmei Wang, Hong Tian, Aidong Liu, Huan Jin, Xiaomei Luo & Yuanshou Chen

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  1. Junwei Zeng
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Zeng, J., Wang, G., Liu, X. et al. P2Y13 Receptor-Mediated Rapid Increase in Intracellular Calcium Induced by ADP in Cultured Dorsal Spinal Cord Microglia. Neurochem Res 39, 2240–2250 (2014). https://doi.org/10.1007/s11064-014-1426-8

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