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Intrathecal miR-96 Inhibits Nav1.3 Expression and Alleviates Neuropathic Pain in Rat Following Chronic Construction Injury

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Abstract

MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally by binding to their cognate target mRNAs. Emerging evidence suggests that miRNAs are critical regulators of neuronal functions. The expression pattern of miRNAs in the peripheral nervous system after peripheral nerve injury suggest that miRNAs may have important and yet unknown roles in the mechanisms of pain. Thus, we examined the role of miR-96 in neuropathic pain using a rat model of the condition chronic constriction sciatic nerve injury (CCI). We found that miR-96 alleviated neuropathic pain. The level of miR-96 was decreased within the ipsilateral dorsal root ganglion (DRG) after peripheral nerve injury but the Nav1.3 level was increased. Specifically, Intrathecal administration of miR-96 suppressed the expression of Nav1.3 induced by CCI. Further examination revealed that miR-96 inhibited the Nav1.3 mRNA expression in the embryonic DRG neurons in vitro. Our findings suggest that miR-96 participate in the regulation of neuropathic pain through inhibiting the expression of Nav1.3 in the DRG of CCI rats.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Nos. 30973030, 30800424, 81260318 and 81371967), Natural Science Foundation (No. 20122BAB205061) and Science and Technology Support Program (No. 2009JX01268) of Jiangxi Province, China. Educational Department Foundation (No. GJJ13155) of Jiangxi Province, China. We are grateful for critical reading of the manuscript by Michael Sun, USA.

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Authors and Affiliations

  1. Department of Histology and Embryology, Medical College, Nanchang University, Bayi Road 603, Nanchang, 330006, China

    Hong-Ping Chen & Lei Zhang

  2. Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guang Zhou Road, Nanjing, 210029, China

    Wei Zhou & Wei-Hua Cai

  3. Department of Animal Science, Medical College, Nanchang University, Bayi Road 603, Nanchang, 330006, China

    Lu-Mei Kang & Bao-Hua Xu

  4. Institute of Quality Safety and Standards of Agricultural Products Research, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Han Yan

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  1. Hong-Ping Chen
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  2. Wei Zhou
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  4. Han Yan
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Correspondence to Bao-Hua Xu or Wei-Hua Cai.

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Hong-Ping Chen, Wei Zhou and Lu-Mei Kang have contributed equally to this study.

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Chen, HP., Zhou, W., Kang, LM. et al. Intrathecal miR-96 Inhibits Nav1.3 Expression and Alleviates Neuropathic Pain in Rat Following Chronic Construction Injury. Neurochem Res 39, 76–83 (2014). https://doi.org/10.1007/s11064-013-1192-z

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  • DOI: https://doi.org/10.1007/s11064-013-1192-z

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