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Hemodialysis Decreases Serum Brain-Derived Neurotrophic Factor Concentration in Humans

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Abstract

In the present study we have evaluated the effect of a single hemodialysis session on the brain-derived neurotrophic factor levels in plasma [BDNF]pl and in serum [BDNF]s as well as on the plasma isoprostanes concentration [F2 isoprostanes]pl, plasma total antioxidant capacity (TAC) and plasma cortisol levels in chronic kidney disease patients. Twenty male patients (age 69.8 ± 2.9 years (mean ± SE)) with end-stage renal disease undergoing maintenance hemodialysis on regular dialysis treatment for 15–71 months participated in this study. A single hemodialysis session, lasting 4.2 ± 0.1 h, resulted in a decrease (P = 0.014) in [BDNF]s by ~42 % (2,574 ± 322 vs. 1,492 ± 327 pg ml−1). This was accompanied by an increase (P < 10−4) of [F2-Isoprostanes]pl (38 ± 3 vs. 116 ± 16 pg ml−1), decrease (P < 10−4) in TAC (1,483 ± 41 vs. 983 ± 35 trolox equivalents, μmol l−1) and a decrease (P = 0.004) in plasma cortisol level (449.5 ± 101.2 vs. 315.3 ± 196.3 nmol l−1). No changes (P > 0.05) in [BDNF]pl and the platelets count were observed after a single dialysis session. Furthermore, basal [BDNF]s in the chronic kidney disease patients was significantly lower (P = 0.03) when compared to the age-matched control group (n = 23). We have concluded that the observed decrease in serum BDNF level after hemodialysis accompanied by elevated [F2-Isoprostanes]pl and decreased plasma TAC might be caused by enhanced oxidative stress induced by hemodialysis.

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Acknowledgments

We thank the patients for their kind cooperation in this study. We also gratefully acknowledge the financial support of the Ministry of Science and Higher Education (Poland) (grant number 245/KFiB/2009) for this project.

Conflict of interest

All the authors declared no competing interests.

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Authors and Affiliations

  1. Department of Physiology and Biochemistry, Faculty of Rehabilitation, University School of Physical Education Kraków, al. Jana Pawla II 78, 31-571, Kraków, Poland

    Jerzy A. Zoladz, Jerzy A. Zoladz, Joanna Majerczak & Justyna Zapart-Bukowska

  2. Dialysis Center Fresenius Nephrocare II, Rydygier Hospital, os. Złotej Jesieni 1, 31-826, Kraków, Poland

    Michał Śmigielski & Olgierd Smoleński

  3. Center of Oncology, M. Sklodowska-Curie Memorial Institute, Kraków Division, ul. Garncarska 11, 31-115, Kraków, Poland

    Łukasz R. Nowak, Jan Kulpa & Krzysztof Duda

  4. Department of Clinical Rehabilitation, Faculty of Rehabilitation, University School of Physical Education Kraków, al. Jana Pawla II 78, 31-571, Kraków, Poland

    Olgierd Smoleński

  5. Department of Molecular Biophysics, University of Łódz, ul. Banacha 12/16, 90-237, Łódz, Poland

    Joanna Drzewińska & Grzegorz Bartosz

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  1. Jerzy A. Zoladz
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Zoladz, J.A., Śmigielski, M., Majerczak, J. et al. Hemodialysis Decreases Serum Brain-Derived Neurotrophic Factor Concentration in Humans. Neurochem Res 37, 2715–2724 (2012). https://doi.org/10.1007/s11064-012-0862-6

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