Skip to main content

Advertisement

SpringerLink
Log in

Effects of Selenium on Calcium Signaling and Apoptosis in Rat Dorsal Root Ganglion Neurons Induced by Oxidative Stress

  • Original Paper
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

Ca2+ is well known for its role as crucial second messenger in modulating many cellular physiological functions, Ca2+ overload is detrimental to cellular function and may present as an important cause of cellular oxidative stress generation and apoptosis. The aim of this study is to investigate the effects of selenium on lipid peroxidation, reduced glutathione (GSH), glutathione peroxidase (GSH-Px), cytosolic Ca2+ release, cell viability (MTT) and apoptosis values in dorsal root ganglion (DRG) sensory neurons of rats. DRG cells were divided into four groups namely control, H2O2 (as a model substance used as a paradigm for oxidative stress), selenium, selenium + H2O2. Moderate doses and times of H2O2 and selenium were determined by MTT test. Cells were preterated 200 nM selenium for 30 h before incubatation with 1 μM H2O2 for 2 h. Lipid peroxidation levels were lower in the control, selenium, selenium + H2O2 groups than in the H2O2 group. GSH-Px activities were higher in the selenium groups than in the H2O2 group. GSH levels were higher in the control, selenium, selenium + H2O2 groups than in the H2O2 group. Cytosolic Ca2+ release was higher in the H2O2 group than in the control, selenium, selenium + H2O2 groups. Cytosolic Ca2+ release was lower in the selenium + H2O2 group than in the H2O2. In conclusion, the present study demonstrates that selenium induced protective effects on oxidative stress, [Ca2+]c release and apoptosis in DRG cells. Since selenium deficiency is a common feature of oxidative stress-induced neurological diseases of sensory neurons, our findings are relevant to the etiology of pathology in oxidative stress-induced neurological diseases of the DRG neurons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Davies MJ (2003) Singlet oxygen-mediated damage to proteins and its consequences. Biochem Biophys Res Commun 305:761–770

    Article  PubMed  CAS  Google Scholar 

  2. Kockar MC, Nazıroğlu M, Celik O et al (2010) N-acetylcysteine modulates doxorubicin-induced oxidative stress and antioxidant vitamin concentrations in liver of rats. Cell Biochem Funct 28:673–677

    Article  PubMed  CAS  Google Scholar 

  3. Nazıroğlu M, Kilinc F, Uguz AC et al (2010) Oral vitamin C and E combination modulates blood lipid peroxidation and antioxidant vitamin levels in maximal exercising basketball players. Cell Biochem Funct 28:300–305

    Article  PubMed  Google Scholar 

  4. Wirth EK, Conrad M, Winterer J et al (2010) Neuronal selenoprotein expression is required for interneuron development and prevents seizures and neurodegeneration. FASEB J 24:844–852

    Article  PubMed  CAS  Google Scholar 

  5. McKenzie RC, Arthur JR, Beckett GJ (2002) Selenium and the regulation of cell signaling, growth, and survival: molecular and mechanistic aspects. Antioxid Redox Signal 4:339–351

    Article  PubMed  CAS  Google Scholar 

  6. Schweizer U, Bräuer AU, Köhrle J, Nitsch R, Savaskan NE (2004) Selenium and brain function: a poorly recognized liaison. Brain Res Rev 45:164–178

    Article  PubMed  CAS  Google Scholar 

  7. Nazıroğlu M (2009) Role of selenium on calcium signaling and oxidative stress-induced molecular pathways in epilepsy. Neurochem Res 34:2181–2191

    Article  PubMed  Google Scholar 

  8. Savas S, Briollais L, Ibrahim-zada I, Jarjanazi H, Choi YH, Musquera M, Fleshner N, Venkateswaran V, Ozcelik H (2010) A whole-genome SNP association study of NCI60 cell line panel indicates a role of Ca2+ signaling in selenium resistance. PLoS ONE 5:e12601

    Article  PubMed  Google Scholar 

  9. Uguz AC, Naziroglu M, Espino J et al (2009) Selenium modulates oxidative stress-induced cell apoptosis in human myeloid HL-60 cells through regulation of calcium release and caspase-3 and -9 activities. J Membr Biol 232:15–23

    Article  PubMed  CAS  Google Scholar 

  10. Wang W, Gu J, Li YQ et al (2011) Are voltage-gated sodium channels on the dorsal root ganglion involved in the development of neuropathic pain? Mol Pain 7:16

    Article  PubMed  CAS  Google Scholar 

  11. Sghirlanzoni A, Pareyson D, Lauria G (2005) Sensory neuron diseases. Lancet Neurol 4:349–361

    Article  PubMed  CAS  Google Scholar 

  12. Devor M (2009) Ectopic discharge in abeta afferents as a source of neuropathic pain. Exp Brain Res 196:115–128

    Article  PubMed  CAS  Google Scholar 

  13. Grynkiewicz C, Poenie M, Tsien RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440–3450

    PubMed  CAS  Google Scholar 

  14. Beales IL, Ogunwobi OO (2010) Microsomal prostaglandin E synthase-1 inhibition blocks proliferation and enhances apoptosis in oesophageal adenocarcinoma cells without affecting endothelial prostacyclin production. Int J Cancer 126:2247–2255

    PubMed  CAS  Google Scholar 

  15. Li GY, Fan B, Zheng YC (2010) Calcium overload is a critical step in programmed necrosis of ARPE-19 cells induced by high-concentration HO. Biomed Environ Sci 23:371–377

    Article  PubMed  CAS  Google Scholar 

  16. Placer ZA, Cushman L, Johnson BC (1966) Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Anal Biochem 16:359–364

    Article  PubMed  CAS  Google Scholar 

  17. Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with ellman’s reagent. Anal Biochem 25:192–205

    Article  PubMed  CAS  Google Scholar 

  18. Lawrence RA, Burk RF (1976) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958

    Article  PubMed  CAS  Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL et al (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  20. Nazıroğlu M, Özgül C, Çiğ B, Doğan S, Uğuz AC (2011) Glutathione modulates Ca(2+) influx and oxidative toxicity through TRPM2 channel in rat dorsal root ganglion neurons. J Membr Biol 242:109–118

    Article  PubMed  Google Scholar 

  21. Ozgül C, Nazıroğlu M (2012) TRPM2 channel protective properties of N-acetylcysteine on cytosolic glutathione depletion dependent oxidative stress and Ca(2+) influx in rat dorsal root ganglion. Physiol Behav 106(2):122–128

    Article  PubMed  Google Scholar 

  22. Chakraborti T, Das S, Mondal M et al (1999) Oxidant, mitochondria and calcium: an overview. Cell Signal 11:77–85

    Article  PubMed  CAS  Google Scholar 

  23. Roy SS, Hajnoczky G (2009) Fluorometric methods for detection of mitochondrial membrane permeabilization in apoptosis. Methods Mol Biol 559:173–190

    Article  PubMed  CAS  Google Scholar 

  24. Liu X, Hajnoczky G (2009) Ca2+-dependent regulation of mitochondrial dynamics by the miro-milton complex. Int J Biochem Cell Biol 41:1972–1976

    Article  PubMed  CAS  Google Scholar 

  25. Koistinaho J, Alho H, Hervonen A (1990) Effect of vitamin E and selenium supplement on the aging peripheral neurons of the male sprague-dawley rat. Mech Ageing Dev 51:63–72

    Article  PubMed  CAS  Google Scholar 

  26. Yuan H, Lan T, Lin J (2005) Modulation of nano-selenium on tetrodotoxin-sensitive voltage-gated sodium currents in rat dorsal root ganglion neurons. Conf Proc IEEE Eng Med Biol Soc 5:4846–4849

    PubMed  Google Scholar 

  27. Yuan H, Lin J, Lan T (2006) Effects of nano red elemental selenium on sodium currents in rat dorsal root ganglion neurons. Conf Proc IEEE Eng Med Biol Soc 1:896–899

    PubMed  Google Scholar 

  28. Vincent AM, Edwards JL, McLean LL et al (2010) Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy. Acta Neuropathol 120:477–489

    Article  PubMed  CAS  Google Scholar 

  29. Fischer LR, Glass JD (2010) Oxidative stress induced by loss of Cu, Zn-superoxide dismutase (SOD1) or superoxide-generating herbicides causes axonal degeneration in mouse DRG cultures. Acta Neuropathol 119:249–259

    Article  PubMed  CAS  Google Scholar 

  30. Nazıroğlu M (2011) TRPM2 cation channels, oxidative stress and neurological diseases: where are we now? Neurochem Res 36:355–366

    Article  PubMed  Google Scholar 

  31. Vincent AM, Hinder LM, Pop-Busui R et al (2009) Hyperlipidemia: a new therapeutic target for diabetic neuropathy. J Peripher Nerv Syst 14:257–267

    Article  PubMed  CAS  Google Scholar 

  32. Fernyhough P, Calcutt NA (2010) Abnormal calcium homeostasis in peripheral neuropathies. Cell Calcium 47:130–139

    Article  PubMed  CAS  Google Scholar 

  33. Uezono Y, Toyohira Y, Yanagihara N, Wada A, Taniyama K (2006) Inhibition by selenium compounds of catecholamine secretion due to inhibition of Ca2+ influx in cultured bovine adrenal chromaffin cells. J Pharmacol Sci 101:223–229

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

MN and ACU formulated the present hypothesis. MN made critical revision to the manuscript. ACU was responsible to make the analysis and writing report. The study was partially supported by the Scientific Research Project Unit of Süleyman Demirel University (1881-D-09). The abstract of the study was submitted in 14th International Symposium on Trace Elements in Man and Animals, Enshi, Hubei, China.

Author information

Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Süleyman Demirel University, Dekanlık Binasi, 32260, Isparta, Turkey

    Abdülhadi Cihangir Uğuz & Mustafa Nazıroğlu

  2. Neuroscience Research Center, Süleyman Demirel University, Isparta, Turkey

    Abdülhadi Cihangir Uğuz & Mustafa Nazıroğlu

Authors
  1. Abdülhadi Cihangir Uğuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mustafa Nazıroğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mustafa Nazıroğlu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uğuz, A.C., Nazıroğlu, M. Effects of Selenium on Calcium Signaling and Apoptosis in Rat Dorsal Root Ganglion Neurons Induced by Oxidative Stress. Neurochem Res 37, 1631–1638 (2012). https://doi.org/10.1007/s11064-012-0758-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11064-012-0758-5

Keywords

Search

Navigation