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Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy

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Abstract

Gelsolin is an actin regulatory protein that generally distributed in a wide variety of body tissues, especially the brain tissues and cerebrospinal fluid. In this study we found that lumbar CSF-gelsolin concentrations markedly decreased in epileptic patients by enzyme linked immunosorbent assay. In order to help judge the result, we determined gelsolin expression in temporal lobe tissues of patients with temporal lobe epilepsy using double-label immunofluorescence to location and using western blot to quantitation. Then we observed that gelsolin was co-expressed with microtubule-associated protein-2 in axons and cytoplasms of neurons and gelsolin protein level was also down-regulated in temporal lobe tissues of epileptic patients. Our findings suggested that CSF-gelsolin level might reflect the alteration of gelsolin in brain tissue of epileptic patients and CSF-gelsolin seems to be a potential biomarker for epilepsy.

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (NO. 30870877). We thank Xuanwu Hospital of Capital Medical University, Guangzhou 39 Brain Hospital, Xinhua Brain Hospital of Hubei Province, Xinqiao Hospital of the Third Military Medical University for their support in brain tissue collection. We also thank the patients for their participation in this study. We confirm that we have read the Journal’s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines.

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Authors and Affiliations

  1. Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 You Yi Road, Chongqing, 400016, China

    Xi Peng, Xiaogang Zhang, Liang Wang, Qiong Zhu, Jing Luo & Xuefeng Wang

  2. Department of Neurology, The Affiliated Hospital of Guiyang Medical College, Guiyang, 550004, Guizhou, China

    Wei Wang

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  1. Xi Peng
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  2. Xiaogang Zhang
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Correspondence to Xuefeng Wang.

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Xi Peng and Xiaogang Zhang contributed equally to this work.

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Peng, X., Zhang, X., Wang, L. et al. Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy. Neurochem Res 36, 2250–2258 (2011). https://doi.org/10.1007/s11064-011-0549-4

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  • DOI: https://doi.org/10.1007/s11064-011-0549-4

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