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An Active Fraction of Achyranthes bidentata Polypeptides Prevents Apoptosis Induced by Serum Deprivation in SH-SY5Y Cells Through Activation of PI3K/Akt/Gsk3β Pathways

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Abstract

Achyranthes bidentata Blume is a commonly prescribed Chinese medicinal herb. Our previous studies have proved the neuroprotective function of Achyranthes bidentata polypeptides (ABPP), a major constituent from aqueous extracts of the herb. Now we have separated an active fraction, referred to as ABPP-E4, from ABPP by HPLC methods. This study aimed to investigate the possible therapeutic potential of ABPP-E4. Assessments of cell viability and apoptosis indicated that ABPP-E4 pretreatment, in a concentration-dependent manner, antagonized the cell viability loss and cell apoptosis of cultured SH-SY5Y cells deprived of serum. ABPP-E4 pretreatment also resulted in increase of Bcl-2/Bax ratio and inhibition of caspase-3 activation in the cells on exposure to serum deprivation. Signaling pathway analysis indicated that ABPP-E4 treatment stimulated the activation of Akt/Gsk3β signaling in cultured SH-SY5Y cells, and anti-apoptotic effects of ABPP-E4 could be blocked by chemical inhibition of PI3K. Taken together, all the results suggest that ABPP-E4 might exert protective effects against serum deprivation-induced neuronal apoptosis through modulation of PI3K/Akt/Gsk3β pathways.

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Acknowledgments

This study was supported by a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions and a project for industrialization promotion of RD achievements in universities and colleges (Grant No. JH09-33). We thank Professor Jie Liu for assistance in manuscript preparation.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, JS, People’s Republic of China

    Yuntian Shen, Qi Zhang, Xiaorong Gao & Fei Ding

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  1. Yuntian Shen
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  2. Qi Zhang
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  3. Xiaorong Gao
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  4. Fei Ding
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Correspondence to Fei Ding.

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Shen, Y., Zhang, Q., Gao, X. et al. An Active Fraction of Achyranthes bidentata Polypeptides Prevents Apoptosis Induced by Serum Deprivation in SH-SY5Y Cells Through Activation of PI3K/Akt/Gsk3β Pathways. Neurochem Res 36, 2186–2194 (2011). https://doi.org/10.1007/s11064-011-0543-x

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  • DOI: https://doi.org/10.1007/s11064-011-0543-x

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