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A Synergistic Role of Hyperthermic and Pharmacological Preconditioning to Protect Astrocytes Against Ischemia/Reperfusion Injury

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Abstract

Recent studies provide solid evidence for the importance to delineate the co-relationship between preconditioning stimuli and therapeutic efficacy of drugs commonly used in clinic. However, very little is known about this important topic. In the present study, we investigated the effects of hyperthermia on the protective role of ginkgolides on astrocytes against ischemia/reperfusion (I/R) injury and also evaluated the effects of the timing of co-treatment of hyperthermia with ginkgolides on astrocytes against the I/R injury. We demonstrated that there is a synergistic action between hyperthermic and pharmacological preconditioning to protect astrocytes against the I/R injury. Our findings also showed that astrocytes have completely different responses to the treatment with hyperthermia or ginkgolides alone or co-treatment together at different stages of the I/R process. Hyperthermic preconditioning before the I/R can protect astrocytes against the I/R injury. However, if treated in the ischemia and reperfusion stage, hyperthermia exacerbates the cell injury and significantly attenuates the protective effectiveness of ginkgolides. These findings imply that the timing of treatment with hypothermia and/or ginkgolides is one of the key factors to determine their protective effects on the cells against the I/R injury.

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Acknowledgments

The studies in our laboratories were supported by The Competitive Earmarked Grants of The Hong Kong Research Grants Council (CUHK466907-KY), NSFC-RGC Joint Research Grant (N-CUHK433/08-KY), Key Project Grant of Jiangsu Province (BG2007607), Grants from Shenzhen-Hong Kong Innovation Circle Programa (2007, 2008, 2009), National Natural Science Foundation of China (30770806, 30971197) and Nantong City Applied Research Grant (K2007021). We declare that we have no financial interests.

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Authors and Affiliations

  1. Institute for Nautical Medicine and Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, People’s Republic of China

    Fang Du, Zhong-ming Qian & Li Zhu

  2. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong

    Fang Du, Xiao Mei Wu, Wing Ho Yung & Ya Ke

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  1. Fang Du
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  2. Zhong-ming Qian
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  3. Li Zhu
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  4. Xiao Mei Wu
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  5. Wing Ho Yung
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  6. Ya Ke
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Correspondence to Ya Ke.

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Du, F., Qian, Zm., Zhu, L. et al. A Synergistic Role of Hyperthermic and Pharmacological Preconditioning to Protect Astrocytes Against Ischemia/Reperfusion Injury. Neurochem Res 36, 312–318 (2011). https://doi.org/10.1007/s11064-010-0327-8

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  • DOI: https://doi.org/10.1007/s11064-010-0327-8

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