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Effects of N-Acetylcysteine/Deferoxamine, Taurine and RC-3095 on Respiratory Chain Complexes and Creatine Kinase Activities in Rat Brain After Sepsis

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Abstract

The pathogenesis of sepsis is characterized by an overwhelming systemic inflammatory response that can lead to multiple organ failure. Considering that we have recently demonstrated that mitochondrial respiratory chain and creatine kinase (CK) are altered in the brain of rats after cecal ligation and perforation (CLP) and that a combination of N-acetylcysteine/deferoxamine (NAC/DFX), taurine and RC-3095 were shown to be an effective treatment of sepsis, we investigated whether the alterations of these enzymes may be reversed by these drugs. The results demonstrated that CLP inhibited complexes I and II, and that all the treatments were able to reverse this inhibition in all brain areas studied in the present work. On the other hand, complexes III and IV were not affected by sepsis neither by any of the treatments. An increase in CK activity in brain of rats 12 h after CLP was also verified; the administration of NAC/DFX and taurine reversed the increase in CK activity in hippocampus, cerebral cortex, cerebellum and striatum. On the other hand, RC-3095 significantly decreased CK activity, when compared to sham group in all brain areas studied. This is a preliminary study which showed beneficial effects of the treatments we proposed.

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Acknowledgments

This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Apoio à Pesquisa Científica e Tecnológica do Estado de Santa Catarina (FAPESC) and Universidade do Extremo Sul Catarinense (UNESC).

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Authors and Affiliations

  1. Laboratório de Fisiopatologia Experimental, Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, 88806-000, Brazil

    Omar J. Cassol Jr, Gislaine T. Rezin, Fabrícia C. Petronilho, Giselli Scaini, Cinara L. Gonçalves, Gabriela K. Ferreira, Felipe Dal-Pizzol & Emilio L. Streck

  2. Grupo de Pesquisa em Neurofarmacologia Celular e Molecular, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90046-900, Brazil

    Rafael Roesler

  3. Laboratório de Pesquisas em Câncer, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil

    Rafael Roesler & Gilberto Schwartsmann

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  1. Omar J. Cassol Jr
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  2. Gislaine T. Rezin
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  3. Fabrícia C. Petronilho
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  9. Felipe Dal-Pizzol
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Correspondence to Emilio L. Streck.

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Cassol, O.J., Rezin, G.T., Petronilho, F.C. et al. Effects of N-Acetylcysteine/Deferoxamine, Taurine and RC-3095 on Respiratory Chain Complexes and Creatine Kinase Activities in Rat Brain After Sepsis. Neurochem Res 35, 515–521 (2010). https://doi.org/10.1007/s11064-009-0089-3

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