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Edaravone Protects Against Apoptotic Neuronal Cell Death and Improves Cerebral Function After Traumatic Brain Injury in Rats

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Abstract

Edaravone is a novel free radical scavenger used clinically in patients with acute cerebral infarction; however, it has not been assessed in traumatic brain injury (TBI). We investigated the effects of edaravone on cerebral function and morphology following TBI. Rats received TBI with a pneumatic controlled injury device. Edaravone (3 mg/kg) or physiological saline was administered intravenously following TBI. Numbers of 8-OHdG-, 4-HNE-, and ssDNA-positive cells around the damaged area after TBI were significantly decreased in the edaravone group compared with the saline group (P < 0.01). There was a significant increase in neuronal cell number and improvement in cerebral dysfunction after TBI in the edaravone group compared with the saline group (P < 0.01). Edaravone administration following TBI inhibited free radical-induced neuronal degeneration and apoptotic cell death around the damaged area. In summary, edaravone treatment improved cerebral dysfunction following TBI, suggesting its potential as an effective clinical therapy.

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Acknowledgment

The authors thank Mari Machino for technical assistance.

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Authors and Affiliations

  1. Department of Pathology, Kinki University School of Medicine, 377-2, Ohno-higashi, Osakasayama, Osaka, 589-8511, Japan

    Tatsuki Itoh, Takao Satou & Hiroyuki Ito

  2. Division of Hospital Pathology, Hospital of Kinki University School of Medicine, Osaka, Japan

    Takao Satou

  3. Division of Sports Medicine, Institute of Life Science, Kinki University, Osaka, Japan

    Takao Satou

  4. Kinki University School of Pharmaceutical Sciences, Osaka, Japan

    Shozo Nishida & Masahiro Tsubaki

  5. Department of Surgery, Kinki University School of Medicine, Osakasayama, Osaka, Japan

    Motohiro Imano

  6. Department of Pathology, PL Hospital, Osaka, Japan

    Shigeo Hashimoto

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  1. Tatsuki Itoh
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  2. Takao Satou
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  3. Shozo Nishida
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  4. Masahiro Tsubaki
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  5. Motohiro Imano
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  7. Hiroyuki Ito
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Correspondence to Tatsuki Itoh.

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Itoh, T., Satou, T., Nishida, S. et al. Edaravone Protects Against Apoptotic Neuronal Cell Death and Improves Cerebral Function After Traumatic Brain Injury in Rats. Neurochem Res 35, 348–355 (2010). https://doi.org/10.1007/s11064-009-0061-2

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  • DOI: https://doi.org/10.1007/s11064-009-0061-2

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