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Presence and Characterization of the Dopamine Transporter in Human Resting Lymphocytes

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Abstract

The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of 3H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of 3H-dopamine (3H-DA). Lymphocytes were obtained by 15 healthy subjects. The results showed the presence of a specific and saturable binding of 3H-WIN 35,428, which labelled one site only. A specific 3H-DA reuptake was also measured. The pharmacological characterization of both binding and reuptake was overlapping. These findings would indicate that human resting lymphocytes carry the DAT, whose functions in periphery are still unknown.

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Authors and Affiliations

  1. Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, University of Pisa, via Roma, 67, 56100, Pisa, Italy

    Donatella Marazziti, Stefano Baroni, Mario Catena Dell’Osso, Irene Masala, Laura Fabbrini, Laura Betti, Gino Giannaccini, Bernardo Dell’Osso & Antonio Lucacchini

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  1. Donatella Marazziti
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  2. Stefano Baroni
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  3. Mario Catena Dell’Osso
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  4. Irene Masala
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  5. Laura Fabbrini
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  6. Laura Betti
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  7. Gino Giannaccini
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  8. Bernardo Dell’Osso
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  9. Antonio Lucacchini
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Correspondence to Donatella Marazziti.

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Marazziti, D., Baroni, S., Catena Dell’Osso, M. et al. Presence and Characterization of the Dopamine Transporter in Human Resting Lymphocytes. Neurochem Res 33, 1011–1016 (2008). https://doi.org/10.1007/s11064-007-9541-4

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  • DOI: https://doi.org/10.1007/s11064-007-9541-4

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