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Tau is Endogenously Nitrated in Mouse Brain: Identification of a Tyrosine Residue Modified In vivo by NO

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Abstract

Nitration of tau protein is normally linked to neurodegeneration but, until now, no comprehensive information is available regarding tau nitration in healthy subjects. It has been previously reported that in differentiated PC12 cells, tau co-immunoprecipitated with alpha-tubulin is nitrated at tyrosine residues and that this post-translation modification doesn’t impair the association of tau with the cytoskeleton. The present paper is focused on the identification of tyrosine residues endogenously modified in tau from PC12 cells and reports for the first time that tau is also nitrated in vivo in normal mouse brain and that one tyrosine is endogenously modified.

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Acknowledgements

We thank Mr. Francesco Corniola for the assistance in figures preparation. This work was supported by grants from FIRST 2003–2004 (University of Milano).

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Authors and Affiliations

  1. D.I.P.A.V. – Section of Biochemistry, University of Milano, Via Celoria 10, Milano, 20100, Italy

    Simona Nonnis, Francesca Taverna, Cristina Ronchi, Severino Ronchi, Armando Negri, Eleonora Grassi & Gabriella Tedeschi

  2. Department of Biology, University of Milano, Via Celoria 26, Milano, 20133, Italy

    Graziella Cappelletti

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  1. Simona Nonnis
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  2. Graziella Cappelletti
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  3. Francesca Taverna
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  4. Cristina Ronchi
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  5. Severino Ronchi
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  6. Armando Negri
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  7. Eleonora Grassi
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  8. Gabriella Tedeschi
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Correspondence to Gabriella Tedeschi.

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Nonnis, S., Cappelletti, G., Taverna, F. et al. Tau is Endogenously Nitrated in Mouse Brain: Identification of a Tyrosine Residue Modified In vivo by NO. Neurochem Res 33, 518–525 (2008). https://doi.org/10.1007/s11064-007-9467-x

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  • DOI: https://doi.org/10.1007/s11064-007-9467-x

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