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Modification of Morphine-induced Hyperlocomotion and Antinociception in Mice by Clorgyline, a Monoamine Oxidase-A Inhibitor

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Abstract

We evaluated the effects of pretreatment with clorgyline, an irreversible monoamine oxidase (MAO)-A inhibitor, on morphine-induced hyperlocomotion and antinociception. A single administration of morphine (30 mg/kg, i.p.) to male ICR mice induced a hyperlocomotion. ANOVA analysis revealed the statistical significance of the morphine effect on horizontal locomotion and of the clorgyline pretreatment × morphine interaction effect, but not of the effect of clorgyline pretreatment. The initial (5 min after challenge) phase of morphine actions vs. saline challenge appeared as if morphine had a strong inhibitory effect on locomotor activity in combination with different doses of clorgyline. The mice administered with morphine in combination of clorgyline (1 and 10 mg/kg) did not show any stereotypic behaviors. Clorgyline at a dose of 0.1 mg/kg but not other doses tested significantly potentiated morphine-induced antinociception evaluated by tail flick but not hot plate test. During the measurements of locomotor activity and antinociception, clorgyline at doses of 1 and 10 mg/kg significantly inhibited monoamine metabolism through MAO. These results suggest that clorgyline showed an inhibitory effect on morphine-induced hyperlocomotion, but not antinociception, through MAO inhibition. There is not a possibility that clorgyline pretreatment enhanced morphine action on motor activity, resulting in the abnormal behavior from hyperlocomotion to stereotypic movements.

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Acknowledgments

NK was supported by a Grant-in-Aid for Researchers, Hyogo College of Medicine.

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  1. Department of Pharmacology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663-8501, Japan

    Nobue Kitanaka, Junichi Kitanaka & Motohiko Takemura

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  1. Nobue Kitanaka
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  2. Junichi Kitanaka
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  3. Motohiko Takemura
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Correspondence to Junichi Kitanaka.

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Kitanaka, N., Kitanaka, J. & Takemura, M. Modification of Morphine-induced Hyperlocomotion and Antinociception in Mice by Clorgyline, a Monoamine Oxidase-A Inhibitor. Neurochem Res 31, 829–837 (2006). https://doi.org/10.1007/s11064-006-9087-x

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  • DOI: https://doi.org/10.1007/s11064-006-9087-x

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