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Immunohistochemical Distribution and Subcellular Localization of the Somatostatin Receptor Subtype 1 (sst1) in the Rat Hypothalamus

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Abstract

The aim of the present study was to examine the cellular and sub-cellular distribution of the somatostatin (SRIF) receptor subtype sst1 in the rat hypothalamus. Receptors were immunolabeled using an antibody directed against an antigenic sequence in the N-terminus of the receptor. Immunopositive neuronal cell bodies and dendrites were observed throughout the mediobasal hypothalamus, including the medial preoptic area, paraventricular, periventricular, and arcuate nuclei. Immunoreactive axons and axon terminals were also observed in the median eminence, suggesting that sst1 is also located pre-synaptically. Electron microscopic examination of the arcuate nucleus revealed a predominant association of immunoreactive sst1 with perikarya and dendrites. Most immunoreactive receptors were intracellular and localized to tubulovesicular compartments and organelles such as the Golgi apparatus, but 14% were associated with the plasma membrane. Of the latter, 47% were apposed to abbuting afferent axon terminals and 20% localized immediately adjacent to an active synaptic zone. These results demonstrate a widespread distribution of sst1 receptors in rat hypothalamus. They also show that somatodendritic sst1 receptors in the arcuate nucleus are ideally poised to mediate SRIF’s modulation of afferent synaptic inputs, including central SRIF effects on growth hormone-releasing hormone neurons documented in this area.

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Acknowledgements

The authors wish to extend their thanks to Mariette Lavallée, Clélia Tommi, Qingyi Chen, and Marwan Samia for expert technical assistance. Ms. Amélie Perron was helpful in performing the immunoblots. This study was supported by Grant MOP−7366 to A.B. and Grant MOP−64328 to G.S.T. from the Canadian Institutes of Health Research; P.S. was recipient of a post-doctoral fellowship by the Fonds de la Recherche en Santé du Québec; G.S.T. holds a career investigator award from the Fonds de la Recherche en Santé du Québec.

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  1. Philippe Sarret

    Present address: Department of Physiology and Biophysics, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Québec, Canada

Authors and Affiliations

  1. Department of Neurology & Neurosurgery, Montreal Neurological Institute, McGill University, 3801, University Street, H3A 2B4, Montréal, Québec, Canada

    Thomas Stroh, Philippe Sarret & Alain Beaudet

  2. Departments of Pediatrics and Neurology & Neurosurgery, Montreal Children’s Hospital Research Institute, McGill University, Montréal, Québec, Canada

    Gloria S. Tannenbaum

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Correspondence to Thomas Stroh.

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Special Issue Dedicated to Miklós Palkovits.

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Stroh, T., Sarret, P., Tannenbaum, G.S. et al. Immunohistochemical Distribution and Subcellular Localization of the Somatostatin Receptor Subtype 1 (sst1) in the Rat Hypothalamus. Neurochem Res 31, 247–257 (2006). https://doi.org/10.1007/s11064-005-9013-7

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