Abstract
The early changes in iron level and neuronal loss in rat nigrostriatal system were investigated using 6-hydroxydopamine (6-OHDA) unilaterally lesioned rats. The results showed that: 1, 3, 5, 7, and 14 days of postlesion, there was a progressive reduction in the density of the tyrosine hydroxylase immunoreactive (TH-ir) cells in the lesioned substantia nigra (SN). Iron level increased in the lesioned SN from 1–14 days following 6-OHDA lesions, but there were no differences in iron level among them. Only on 14 days of postlesion, did the DA release decrease in striatum (Str) of the lesioned side, while there were no changes in other groups. These results implied that the increased iron level in SN occured when there was a moderate reduction of DA neurons. However, the DA release in Str was unchanged until TH-ir cells were highly reduced due to the immense compensatory mechanism of the DA system.
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References
D. Venarucci V. Venarucci A. Vallese L. Battila A. Casado R. De la Torre M. E. Lopez Fernandez (1999) ArticleTitleFree Radicals: Important cause of pathologies refer to aging Panminerva Med. 41 335–339
E. C. Hirsch B. A. Faucheux (1998) ArticleTitleIron metabolism and Parkinson’s disease Mov. Disord. 13 39–45
R. Deumens A. Blokland J. Prickaerts (2002) ArticleTitleModeling Parkinson’s disease in rats: An evaluation of 6-OHDA lesions of the nigrosriatal pathway Exp. Neurol. 175 303–317
Y. Zhou Z. Q. Zhao J. X. Xie (2001) ArticleTitleEffects of isatin on rotational behavior and DA levels in caudate putamen in Parkinsonian rats Brain Res. 917 127–132
C. D. Earl T. Reum Junxia Xie J. Sautter A. Kupsch W. H. Oertel R. Morgenstern (1996) ArticleTitleFoetal nigral cell suspension grafts influence dopamine release in the non-grafted side in the 6-OHDA rat model of Parkinson’s disease: In vivo voltammetrice data Exp. Brain Res. 106 179–184
J. X. Xie H. Jiang W. F. Chen Z. M. Qian (2003) ArticleTitleDopamine release rather than content in the caudate putamen is associated with behavioral changes in the iron rat model of Parkinson’s disease Exp. Neurol. 182 483–489
J. X. Xie Y. Zhou H. Jiang (2000) ArticleTitleMonitoring dopamine release by fast cyclic voltammetry: An in vivo study Chin. J. Appl. Physiol. 16 370–372
H. Jiang W. F. Chen J. X. Xie (2001) ArticleTitleRelationship between dopamine and iron contents in the brain of Parkinsonian rats Acta Physiol. sin. 53 334–338
C. M. Morris J. M. Candy A. E. Oakley C. A. Bloxham J. A. Edwardson (1992) ArticleTitleHistochemical distribution of non-haem iron in the human brain Acta Anat (Basel). 144 235–257
E. Kienzl K. Jellinger H. Stachelberger W. Linert (1999) ArticleTitleIron as catalyst for oxidative stress in the pathogenesis of Parkinson’s disease Life Sci. 65 1973–1976
D. Ben-Shachar P. Riederer M. B. Youdim (1991) ArticleTitleIron-melanine interaction and lipid peroxidation: Implications for Parkinson’s disease J. Neurochem. 57 1609–1614
S. Sastry G. W. Arendash (1995) ArticleTitleTime-dependant changes in iron levels and associated neuronal loss within the substantia nigra following lesions within the neostriatum/globus pallidus complex Neurosci. 67 649–66
J. J. O’Connor Z. L. Kruk (1991) ArticleTitleFast cyclic voltammetry can be used to meassure stimulated endogenous 5-hydroxytamine release in untreated rat brain slices J. Neurosci. Methods. 38 25–33
M. J. Zigmond E. M. Stricker (1973) ArticleTitleRecovery of feeding and drinking by rats after intraventricular 6-hydroxydopamine or lateral hypothalamic lesions Science. 182 717–720
H. Bernheimer W. Birkmayer O. Hornykiewicz K. Jellinger F. Seitelberger (1973) ArticleTitleBrain dopamine and the syndromes of Parkinson and Huntington: Clinical, morphological and neurochemical correlations J. Neurol. Sci. 20 415–455
W. Q. Zhang H. A. Tilson K. P. Nanry P. M. Hudson J. S. Hong M. K. Stachowiak (1988) ArticleTitleIncreased dopamine release from striata of rats after unilateral nigrostriatal boundle damage Brain Res. 461 335–342
P. Riederer E. Sofic W. D. Rausch B. Schmidt G. P. Reynolds K. Jellinger M. B. Youdim (1989) ArticleTitleTransition metals, ferritin, glutathion and ascorbic acid in Parkinsonian brains J. Neurochem. 52 515–520
D. Berg M. Gerlach M. B. Youdim K. L. Double L. Zecca P. Riederer G. Becker (2001) ArticleTitleBrain iron pathways and their relevance to Parkinson’s disease J. Neurochem. 79 225–236
T. A. Rouault (2001) ArticleTitleIron on the brain Nat. Genet. 28 299–300
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Wang, J., Jiang, H. & Xie, JX. Time Dependent Effects of 6-OHDA Lesions on Iron Level and Neuronal Loss in Rat Nigrostriatal System. Neurochem Res 29, 2239–2243 (2004). https://doi.org/10.1007/s11064-004-7031-5
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DOI: https://doi.org/10.1007/s11064-004-7031-5