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Evaluation of radiation necrosis and malignant glioma in rat models using diffusion tensor MR imaging

  • Laboratory Investigation - Human/Animal Tissue
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Abstract

Standard MRI cannot distinguish between radiation necrosis and tumor progression; however, this distinction is critical in the assessment of tumor response to therapy. In this study, one delayed radiation necrosis model (dose, 40 Gy; radiation field, 10 × 10 mm2; n = 13) and two orthotopic glioma models in rats (9L gliosarcoma, n =8; human glioma xenografts, n = 5) were compared using multiple diffusion tensor imaging (DTI) indices. A visible isotropic apparent diffusion coefficient (ADC) pattern was observed in the lesion due to radiation necrosis, which consisted of a hypointense central zone and a hyperintense peripheral zone. There were significantly lower ADC, parallel diffusivity, and perpendicular diffusivity in the necrotic central zone than in the peripheral zone (all P < 0.001). When radiation-induced necrosis was compared with viable tumor, radiation necrosis had significantly lower ADC than 9L gliosarcoma and human glioma xenografts (both P < 0.01) in the central zone, and significantly lower fractional anisotropy than 9L gliosarcoma (P = 0.005) and human glioma xenografts (P = 0.012) in the peripheral zone. Histological analysis revealed parenchymal coagulative necrosis in the central zone, and damaged vessels and reactive astrogliosis in the peripheral zone. These data suggest that qualitative and quantitative analysis of the DTI maps can provide useful information by which to distinguish between radiation necrosis and viable glioma.

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Acknowledgments

The authors thank Dr. Hangyi Jiang for helpful discussion and Ms. Mary McAllister for editorial assistance. This work was supported in part by grants from NIH (EB009112 and EB009731) and the Dana Foundation.

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Authors and Affiliations

  1. Department of Radiology, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, 336 Park Building, Baltimore, MD, 21287, USA

    Silun Wang, Yifei Chen, Kun Yan & Jinyuan Zhou

  2. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA

    Bachchu Lal & John Laterra

  3. Department of Radiation Oncology, Kennedy Krieger Institute, Baltimore, MD, 21205, USA

    Eric Ford, Erik Tryggestad & Michael Armour

  4. Department of Neurology, Kennedy Krieger Institute, Baltimore, MD, 21205, USA

    Bachchu Lal & John Laterra

  5. F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, 21205, USA

    Jinyuan Zhou

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  1. Silun Wang
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Correspondence to Jinyuan Zhou.

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Wang, S., Chen, Y., Lal, B. et al. Evaluation of radiation necrosis and malignant glioma in rat models using diffusion tensor MR imaging. J Neurooncol 107, 51–60 (2012). https://doi.org/10.1007/s11060-011-0719-x

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  • DOI: https://doi.org/10.1007/s11060-011-0719-x

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