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Activity of lysosomal exoglycosidases in human gliomas

  • Laboratory Investigation
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Abstract

There is a lot of data suggesting that modifications of cell glycoconjugates may be important in progression of cancer. In the present work we studied activities of lysosomal exoglycosidases: β-hexosaminidase and its isoenzymes A and B, β-galactosidase and α-mannosidase, in human gliomas. Enzyme activity was determined spectrophotometrically based on the release of p-nitrophenol from p-nitrophenyl-derivative of appropriate sugars. The activities of the exoglycosidases tested were significantly higher in malignant glial tumors than in control tissue (normal brain tissue) and non-glial tumors. The highest activities of exoglycosidases were observed in high-grade gliomas, and a positive correlation of enzyme activities and degree of malignancy was noted. Our results suggest that lysosomal exoglycosidases may participate in the progression and dynamical development of glial tumors.

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Acknowledgement

This work was supported by a grand from Medical University of Bialystok.

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Authors and Affiliations

  1. Department of Pharmaceutical Biochemistry, Medical University of Bialystok, ul. Mickiewicza 2A , 15-089, Bialystok, Poland

    P. Wielgat, U. Walczuk, S. Szajda & K. Zwierz

  2. Department of Neurosurgery, Medical University of Bialystok, Bialystok, Poland

    M. Bień & Z. Mariak

  3. Department of Medical Pathomorphology, Medical University of Bialystok, Bialystok, Poland

    L. Zimnoch

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  1. P. Wielgat
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  2. U. Walczuk
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Correspondence to P. Wielgat.

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Wielgat, P., Walczuk, U., Szajda, S. et al. Activity of lysosomal exoglycosidases in human gliomas. J Neurooncol 80, 243–249 (2006). https://doi.org/10.1007/s11060-006-9188-z

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  • DOI: https://doi.org/10.1007/s11060-006-9188-z

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