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Mini-cuttings: an effective technique for the propagation of Pinus pinaster Ait.

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Abstract

Pinus pinaster Ait. is one of the main forest tree species planted in Spain, Portugal and France. Due to its high economic relevance, there is considerable interest in developing techniques for vegetative breeding aimed at mass propagation. In this study we present a mini-propagation protocol in order to define an efficient method to propagate families or clones of P. pinaster. We carried out three experiments using mini-cuttings of 3–5 cm in length with the aim of evaluating the effects of temperature (4°C vs. 25°C), plant growth regulator (IBA) and shoot age on rooting ability. Percentage of rooted cuttings and morphological root variables were recorded. The percentage of rooted cuttings per treatment ranged from 68 to 97%. Treatment with IBA significantly influenced the rooting process at 25°C but not at 4°C. The number of apexes, length, area and volume of roots were all positively affected by temperature treatment. Shoot age also had a positive effect on rooting capacity of cuttings, with the cuttings from the youngest shoots (70 days after pruning) having higher rooting percentages, ranging from 84.7 to 98.3%. The use of juvenile material, good environmental conditions and IBA all benefited the rooting of clonal material, resulting in high rooting capacity. This study presents an innovative propagation protocol for P. pinaster that can be used as a tool in breeding programs.

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Acknowledgments

This study was supported by funding from the European Project TREESNIP-QLK3-CT2002-01973 and the project “Heterogeneidad ambiental y adaptabilidad en respuesta a la sequía en colecciones clonales de Pinus pinaster”, ref RTA-2007-00084-00-00. Enrique Fernández, Dolores Busto, Inmaculada Concepción and Manuel Zapico provided technical support during the experiments.

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Authors and Affiliations

  1. Sección Forestal, SERIDA, Finca Experimental “La Mata”, 33820, Grado Principado de Asturias, Spain

    Juan Majada, Isabel Feito & Angelo Kidelman

  2. Sección Forestal, CETEMAS, Finca Experimental “La Mata”, 33820, Grado Principado de Asturias, Spain

    Juan Majada & Celia Martínez-Alonso

  3. Departamento de Sistemas y Recursos Forestales, CIFOR, Instituto Nacional de Investigación y Tecnología Agraria (INIA), Carretera de La Coruña, Km. 7.5, 28040, Madrid, Spain

    Ismael Aranda & Ricardo Alía

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  1. Juan Majada
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  2. Celia Martínez-Alonso
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  3. Isabel Feito
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  4. Angelo Kidelman
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  5. Ismael Aranda
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Correspondence to Celia Martínez-Alonso.

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Majada, J., Martínez-Alonso, C., Feito, I. et al. Mini-cuttings: an effective technique for the propagation of Pinus pinaster Ait.. New Forests 41, 399–412 (2011). https://doi.org/10.1007/s11056-010-9232-x

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  • DOI: https://doi.org/10.1007/s11056-010-9232-x

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