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Growth response of Acacia mangium Willd. seedlings to arbuscular mycorrhizal fungi and four isolates of the ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker and Couch

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Abstract

Some Acacia mangium Willd. plantations in Asia grow poorly due to low soil fertility and the absence of compatible mycorrhizal fungi. This legume tree can be colonized by arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) fungi but inoculation is not routinely practiced. This study aimed to compare the effectiveness of AM fungi and four isolates of the ECM fungus Pisolithus tinctorius (Pers.) Coker and Couch (Pt) in promoting growth of A. mangium seedlings under glasshouse conditions. AM inoculants were: Glomus etunicatum, G. fasciculatum, G. macrocarpum and Gigaspora margarita and mixed species extracted from rhizosphere soil of a Populus stand in Suwon (AMKFRI), and Carex (AMM6) and Populus (AMM7) growing in mine tailings in Korea. Pisolithus isolates were from Philippines (PtPhil) and Korea (PtKFRI, PtMKACC, PtKACC). Generally, ECM fungi promoted height and diameter growth of A. mangium more than the AM inoculants. The Korean Pisolithus increased plant dry weight by 122–145%, mixed AM inoculants by 61–97%, and Glomus and Gigaspora by 45–72% over the control. PtKACC gave the highest root colonization and promoted the highest growth and concentration of most nutrients. Mycorrhizal root colonization was positively correlated with plant dry weight, Na, Fe and Cu concentrations and N, P, K, Ca, Na, Fe and Cu contents. In conclusion, the results provide strong evidence for benefits of mycorrhizal inoculation on A. mangium seedlings under glasshouse conditions. The Korean Pisolithus isolates (particularly PtKACC), and two AM fungi (AMKFRI and AMM6) are potential mycorrhizal inoculants but their effectiveness and persistence should be determined on degraded lands in tropical countries where A. mangium is being planted for rehabilitation.

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Acknowledgments

This study was conducted while the senior author was on Post Doctoral Fellowship at the Korea Forest Research Institute, Suwon, Korea. The authors acknowledge Dr. Kang-Hyeon Ka (KFRI, Seoul), Rural Development Administration (Korea Agricultural Culture Collection and Mushroom Korea Agricultural Culture Collection) for the ECM cultures.

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Authors and Affiliations

  1. Biotechnology Division, Korea Forest Research Institute, 44-3 Omokcheon, Gwonseon, Suwon, Gyeonggi, 441-350, South Korea

    Nelly Siababa Aggangan & Heung Kyu Moon

  2. National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Baños, 4031, College, Los Baños, Laguna, Philippines

    Nelly Siababa Aggangan

  3. Forest Genetic Resources Division, Korea Forest Research Institute, 44-3 Omokcheon, Gwonseon, Suwon, Gyeonggi, 441-350, South Korea

    Sim Hee Han

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  1. Nelly Siababa Aggangan
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  2. Heung Kyu Moon
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  3. Sim Hee Han
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Correspondence to Nelly Siababa Aggangan.

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Aggangan, N.S., Moon, H.K. & Han, S.H. Growth response of Acacia mangium Willd. seedlings to arbuscular mycorrhizal fungi and four isolates of the ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker and Couch. New Forests 39, 215–230 (2010). https://doi.org/10.1007/s11056-009-9165-4

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  • DOI: https://doi.org/10.1007/s11056-009-9165-4

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