Abstract
Acacia mangium grown in aeroponic culture was co-inoculated with selected strains of Bradyrhizobium sp. and Glomus intraradices. A single-step technique using alginate as an embedding and sticking agent for an inoculum composed of arbuscular mycorrhiza (AM)-infected sheared roots was used to infect plants. This method resulted in the successful establishment of AM in 100% of the inoculated plants after 7 weeks. The results indicated that dual microbial inoculation with Glomus intraradices strain S-043 and Bradyrhizobium strain AUST 13C stimulated the growth of A. mangium in aeroponic culture. The effects of single and dual microbial inoculations were also evaluated at two levels of P in the nutrient medium. A concentration of 5 mg P kg−1 stimulated the development of AM without affecting plant development or establishment of Bradyrhizobium symbiosis. In contrast, saplings supplemented with a higher concentration of P (25 mg kg−1) alone or co-inoculated with Bradyrhizobium had lower AM frequencies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahmad M (1995) Compatibility and co-selection of vesicular–arbuscular mycorrhizal fungi and rhizobia for tropical legumes. Crit Rev Biotechnol 15:229–239
Allen SE (1989) Analysis of vegetation and other organic materials. In: Allen SE (ed) Chemical analysis of ecological materials. Blackwell, Oxford, pp 46–61
Awang K, Taylor DA (1991) Tropical acacias in East Asia and Pacific. Winrock, Bangkok
Awang K, Taylor DA (1993) Acacia mangiumg growth and utilization. (MPTS monograph series no. 3) Winrock, FAO, Bangkok
Barr C (2001) Banking on sustainability structural adjustment and forestry reform in post-Suharto Indonesia. Centre for International Forestry Research, Bogor Barat, Indonesia
Bashan Y, Hernandez JP, Leyva LA, Bacilio M (2002) Alginate microbeads as inoculant carriers for plant growth-promoting bacteria. Biol Fertil Soils 35:359–368
Bayne HG, Brown MS, Bethlenfalvay GJ (1984) Defoliation effects on mycorrhizal colonization, nitrogen fixation and photosynthesis in the Glycine–Glomus–Rhizobium symbiosis. Physiol Plant 62:576–580
Bethlenfalway GJ, Brown MS, Stafford AE (1985) Glycine–Glomus–Rhizobium symbiosis. Plant Physiol 79:1054–1058
Catford JG, Staehlin C, Lerat S, Piché Y, Vierheilig H (2003) Suppression of arbuscular mycorrhizal colonization and nodulation in split-root systems of alfalfa after pre-inoculation and treatment with nod factors. J Exp Bot 54:1481–1487
Dommergues Y, Duhoux E, Diem HG (1999) Les arbres fixateurs d’azote. CIRAD, ESPACE34, FAO, IRD, Montpellier
Ducousso M, Colonna JP, Badji S, Thoen D (1991) Influence de l’azote et du phosphore sur l’établissement de la symbiose quadripartite: Acacia holosericea/Bradyrhizobium sp./Glomus mosseae/Pisolithus sp. In: Riedacker A, Dreyer E, Pafadnam C, Joly H, Bary G (eds) Physiologie des arbres et arbustes en zones arides et semi-arides. Libbey Eurotext, Paris, pp 215–228
Galiana A, Chaumont J, Diem HG, Dommergues YR (1990) Nitrogen-fixing potential of Acacia mangium and Acacia auriculiformis seedlings inoculated with Bradyrhizobium and Rhizobium spp. Biol Fertil Soils 9:261–267
Galiana A, Prin Y, Mallet B, Gnahoua GM, Poitel M, Diem HG (1994) Inoculation of Acacia mangium with alginate beads containing selected Bradyrhizobium strains under field conditions: long-term effect on plant growth and persistence of the introduced strains in soil. Appl Environ Microbiol 60:3974–3980
Galiana A, Gnahoua GM, Chaumont J, Lesueur D, Prin Y, Mallet B (1998) Improvement of nitrogen fixation in Acacia mangium through inoculation with Rhizobium. Agrofor Syst 40:297–307
Galiana A, Balle P, N’Guessan Kanga A, Domenach AM (2002) Nitrogen fixation estimated by the 15N natural abundance method in Acacia mangium Willd. inoculated with Bradyrhizobium sp. and grown in silvicultural conditions. Soil Biol Biochem 34:251–262
Hattingh MJ, Gray LE, Gerdemann JW (1973) Uptake and translocation of 32P-labelled phosphate to onion roots by endomycorrhizal fungi. Soil Sci 116:383–387
Hung LL, Sylvia DM (1988) Production of vesicular–arbuscular mycorrhizal fungus inoculum in aeroponic culture. Appl Environ Microbiol 54:353–357
Hung LL, O’Keefe DM, Sylvia DM (1991) Use of hydrogel as a sticking agent and carrier for vesicular–arbuscular mycorrhizal fungi. Mycol Res 95:427–429
Jung G, Mugnier J, Diem HG, Dommergues YR (1982) Polymer-entrapped Rhizobium as an inoculant for legumes. Plant Soil 65:219–231
Koske RE, Testier B (1983) A convenient permanent slide mounting medium. Newslett Mycol Soc Am 34:59
Kuek C, Tommerup IC, Malajczuk N (1992) Hydrogel beads inocula for the production of ectomycorrhizal eucalypts for plantations. Mycol Res 96:273–277
Lewis D, Quirk JP (1967) Phosphate diffusion in soil and uptake by plants. IV. Computed uptake by model roots as a result of diffusive flow. Plant Soil 26:454–468
Martin-Laurent F, Lee SK, Tham FY, He J, Diem HG, Durand P (1997) A new approach to enhance growth and nodulation of Acacia mangium through aeroponic culture. Biol Fertil Soils 5:7–12
Martin-Laurent F, Frémont M, Lee SK, Tham FY, Prin Y, Tan TK, Diem HG (1999) Effect of inoculation with selected Bradyrhizobium spp. on the survival and growth of Acacia mangium saplings after 20 months in the field. J Trop For Sci 11:470–483
Pearson JN, Abbot LK, Jasper DA (1993) Mediation of competition between two colonizing VA mycorrhizal fungi by the host plant. New Phytol 123:93–98
Pella E (1990) Elemental organic analysis. Part 1. Historical developments. Am Lab 22:116–125
Prin Y, Neyra M, Ducousso M, Dommergues YR (1989) Viabilité d’un inoculum déterminée par l’activité réductrice de l’INT. Agron Trop 44:13–19
Prin Y, Galiana A, Le Roux C, Méléard B, Razafimaharo V, Ducousso M, Chaix G (2003) Molecular tracing of Bradyrhizobium strains helps to correctly interpret Acacia mangium response to inoculation in a reforestation experiment in Madagascar. Biol Fertil Soils 37:64–69
Requena N, Jimenez I, Toro M, Barea JM (1997) Interactions between plant-growth-promoting rhizobacteria (PGPR), arbuscular mycorrhizal fungi and Rhizobium spp. in the rhizosphere of Anthyllis cytisoides, a model legume for revegetation in mediterranean semi-arid ecosystems. New Phytol 136:667–677
Ribet J, Drevon JJ (1996) The phosphorus requirement of N2-fixing and urea fed Acacia mangium. New Phytol 132:383–390
Santiago GM, Garcia Q, Scotti MR (2002) Effect of post-planting inoculation with Bradyrhizobium sp and mycorrhizal fungi on the growth of Brazilian rosewood, Dalbergia nigra Allem. ex Benth., in two tropical soils. New For 24:15–25
Smith SE, Read JD (1997) Mycorrhizal symbiosis, 2nd edn. Academic Press, USA
Sougoufara B, Diem HG, Dommergues YR (1989) Response of field-grown Casuarina equisetifolia to inoculation with Frankia strain ORS 021001 entrapped in alginate beads. Plant Soil 118:133–139
Sylvia DM, Jarstfer AG (1992) Sheared-root inocula of vesicular–arbuscular mycorrhizal fungi. Appl Environ Microbiol 58:2229–2232
Toro M, Azcon R, Barea JM (1997) Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate-solubilizing rhizobacteria to improve rock phosphate availability (32P) and nutrient cycling. Appl Environ Microbiol 63:4408–4412
Trouvelot A, Kough J, Gianinazzi-Pearson V (1986) Mesure du taux de mycorrhization VA d’un système radiculaire. Recherche de methodes d’estimation ayant une signification fonctionnelle. In: Mycorrhizae: physiology and genetics. First European Symposium on Mycorrhizas, Dijon, 1–5 July 1985. pp 217–222
Tsimilli-Michael M, Eggenberg P, Biro B, Köves-Pechy K, Vörös I, Strasser RJ (2000) Synergistic and antagonistic effects of arbuscular mycorrhizal fungi and Azospirillum and Rhizobium nitrogen-fixers on the photosynthetic activity of alfalfa, probed by the polyphasic chlorophyll a fluorescence transient O-J-I-P. Appl Soil Ecol 15:169–182
Turnbull JW (1987) Australian acacias in developing countries. (ACIAR proceedings, vol 16.) Australian Centre for International Agricultural Research, Canberra
Vadez V, Lim G, Durand P, Diem HG (1995) Comparative growth and symbiotic performance of four Acacia mangium provenances from Papua New Guinea in response of the supply of phosphorus at various concentrations. Biol Fertil Soils 19:60–64
van Elsas JD, Trevors JT, Jain D, Wolters AC, Heijnen CE, van Overbeek LS (1992) Survival of, and colonization by, alginate-encapsulated Pseudomonas fluorescens cells following introduction into soils. Biol Fertil Soils 14:14–22
Vassilev N, Vassileva M, Azcon R, Medina A (2001) Interactions of an arbuscular mycorrhizal fungus with free or co-encapsulated cells of Rhizobium trifolii and Yarowia lipolytica inoculated into a soil-plant system. Biotechnol Lett 23:149–151
Xavier LJC, Germida JJ (2002) Response of lentil under controlled conditions to co-inoculation with arbuscular mycorrhizal fungi and rhizobia varying in efficacy. Soil Biol Biochem 34:181–188
Acknowledgements
The authors are grateful to Nanyang Technological University, Singapore for providing a postgraduate research scholarship to Jean Weber and to the French Ministry of Foreign Affairs for financially supporting the research.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Weber, J., Ducousso, M., Tham, F.Y. et al. Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture. Biol Fertil Soils 41, 233–239 (2005). https://doi.org/10.1007/s00374-005-0833-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00374-005-0833-z