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Transgenic poplar with enhanced growth by introduction of the ugt and acb genes

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Abstract

The transgenic poplar (Populus tremula L.) was obtained by transfer of the ugt and acb genes via triparental mating, which was employed to deliver large fragments of TDNA as a cluster. Freshly harvested seeds of local poplar were placed on MS agar medium and plantlets were obtained. After 1 year of subcultivation, plantlets were infected with a transconjugant of triparental mating with target ugt and acb genes into axillary buds. The transformed sprouts so obtained were cut and subcultivated on agar medium with an addition of 0.6 mg/l indole-3-butyric acid as an auxin source. The transformed sprouts showed GUS activity and resistance to gentamycin and kanamycin. The integrity of the target ugt and acb genes into poplar genome was demonstrated via PCR and Southern blot hybridisation. The transgenic poplar plants revealed a higher growth energy, corresponding to a higher content of IAA as opposed to control plants. Both transgenic and non-transformed plants were potted into soil for outdoor acclimatisation and subsequently transferred to earth in beds. Growing outside during 3 years, the transgenic poplar demonstrated a higher growth rate with fast bud and branch development.

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Abbreviations

ugt :

the gene encoding the synthesis of uridine 5′-diphosphoglucosyl transferase

acb :

the gene encoding the synthesis of acyl-CoA-binding protein

UDPG-transferase:

uridine diphosphoglycosyltransferase

IAA:

indoleacetic acid

IBA:

indolebutyric acid

MUG:

4-methylumbelliferyl β-d-glucuronide

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of Russian Academy of Sciences, Lermontov Str., 132, Irkutsk, 664033, Russia

    Rurick Salyaev, Natalya Rekoslavskaya & Anastasia Chepinoga

  2. Istituto Biologia Biotecnologia Agraria, C.N.R., via Bassini, 15, Milan, 20133, Italy

    Sergio Mapelli

  3. Agricultural Service of California, Los Angeles, USA

    R. Pacovsky

Authors
  1. Rurick Salyaev
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  2. Natalya Rekoslavskaya
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  3. Anastasia Chepinoga
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  4. Sergio Mapelli
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  5. R. Pacovsky
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Correspondence to Sergio Mapelli.

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Salyaev, R., Rekoslavskaya, N., Chepinoga, A. et al. Transgenic poplar with enhanced growth by introduction of the ugt and acb genes. New Forest 32, 211–229 (2006). https://doi.org/10.1007/s11056-005-6100-1

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  • DOI: https://doi.org/10.1007/s11056-005-6100-1

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