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Positively charged biopolymeric nanoparticles for the inhibition of Pseudomonas aeruginosa biofilms

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Abstract

Currently, many microbial infections have the potential to become lethal owing to the development of antimicrobial resistance by means of different mechanisms and mainly on the basis of the fact that many drugs are unable to reach therapeutic levels in the target sites. This requires the use of high doses and frequent administrations, causing adverse side effects or in some cases toxicity. The use of nanoparticle systems could help overcome such problems and increase drug efficacy. In the present study, we developed a new drug delivery system based on the use of biopolymeric nanovectors loaded with tobramycin (Tb), which is the standard antibiotic for the treatment of Cystic Fibrosis-associated P. aeruginosa lung infections. Tb-loaded biopolymeric nanoparticles composed by dextran sulfate (DS) and chitosan (CS) were prepared by ionotropic gelation. We optimized drug entrapment in DS/CS nanoparticles, obtaining particles of 170 nm and with a drug loading of 400 µg Tb/mg of nanoparticles. In accord with in vitro release experiments, such preparations were able to release approximately 25 % of their cargo in 60 h. In vitro, the antimicrobial efficacy of the drug delivery system on P. aeruginosa biofilm was tested and compared to the effects of free drug revealing that this formulation can reduce the viability of P. aeruginosa biofilms for 48 h with a single-dose administration.

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Acknowledgments

This work was supported by Sapienza University Grants N C26A13YST and C26A1543KX to FA. Enea Gino Di Domenico was supported by L’Associazione Nazionale Contro le Infezioni Ospedaliere (L’ANCIO).

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    Authors and Affiliations

    1. Department of Chemistry, University of Rome La Sapienza, Rome, Italy

      Laura Chronopoulou & Cleofe Palocci

    2. Department of Clinical Pathology and Microbiology, San Gallicano Institute, IRCCS, Rome, Italy

      Enea Gino Di Domenico

    3. Department of Biology and Biotechnology C. Darwin, University of Rome La Sapienza, Rome, Italy

      Fiorentina Ascenzioni

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    1. Laura Chronopoulou
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    2. Enea Gino Di Domenico
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    3. Fiorentina Ascenzioni
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    4. Cleofe Palocci
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    Correspondence to Cleofe Palocci.

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    The authors declare no conflict of interest.

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    Laura Chronopoulou and Enea Gino Di Domenico have contributed equally to the work.

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    Chronopoulou, L., Di Domenico, E.G., Ascenzioni, F. et al. Positively charged biopolymeric nanoparticles for the inhibition of Pseudomonas aeruginosa biofilms. J Nanopart Res 18, 308 (2016). https://doi.org/10.1007/s11051-016-3611-y

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