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Fe3O4 nanoparticles engineered for plasmid DNA delivery to Escherichia coli

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Abstract

Heat shock treatment is the most popular method for transformation of Escherichia coli. We have used 19-nm Fe3O4 nanoparticles for improving heat shock protocol. PGEM-T (3,000 bp) and pCAMBIA (8,428 bp) were used as test plasmids for transformation of competent E. coli cells (strains DH5α and Jm107) obtained from heat shock- and CaCl2-treated bacteria. A combination of heat shock and Fe3O4 nanoparticles led to a significant increase (6–10 fold) in number of transformed colonies in comparison with heat shock alone. The percent increase in transformation efficiency was higher for larger pCAMBIA plasmids compared to PGEM-T. The transformation efficiency decreased in the absence of CaCl2 and increased by addition of glycerol to the bacterial culture.

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Acknowledgments

Authors are grateful to Sara Saei for editing the manuscript.

Conflict of interest

The authors declare no conflict of interests.

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Author notes

  1. Amir Ata Saei

    Present address: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

Authors and Affiliations

  1. Faculty of Pharmacy, The School of Advanced Biomedical Sciences (SABS), Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, 5165665811, Tabriz, Iran

    Amir Ata Saei, Abolfazl Barzegari, Davoud Asgari & Yadollah Omidi

  2. Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran

    Mostafa Heidari Majd

Authors
  1. Amir Ata Saei
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  2. Abolfazl Barzegari
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  3. Mostafa Heidari Majd
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  5. Yadollah Omidi
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Correspondence to Yadollah Omidi.

Additional information

Amir Ata Saei and Abolfazl Barzegari contributed equally to this work.

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Saei, A.A., Barzegari, A., Majd, M.H. et al. Fe3O4 nanoparticles engineered for plasmid DNA delivery to Escherichia coli . J Nanopart Res 16, 2521 (2014). https://doi.org/10.1007/s11051-014-2521-0

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