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PAMAM dendrimer-coated iron oxide nanoparticles: synthesis and characterization of different generations

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Abstract

This study focuses on the synthesis and characterization of different generations (G0–G7) of polyamidoamine (PAMAM) dendrimer-coated magnetic nanoparticles (DcMNPs). In this study, superparamagnetic iron oxide nanoparticles were synthesized by co-precipitation method. The synthesized nanoparticles were modified with aminopropyltrimethoxysilane for dendrimer coating. Aminosilane-modified MNPs were coated with PAMAM dendrimer. The characterization of synthesized nanoparticles was performed by X-ray diffraction, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), dynamic light scattering, and vibrating sample magnetometry (VSM) analyses. TEM images demonstrated that the DcMNPs have monodisperse size distribution with an average particle diameter of 16 ± 5 nm. DcMNPs were found to be superparamagnetic through VSM analysis. The synthesis, aminosilane modification, and dendrimer coating of iron oxide nanoparticles were validated by FTIR and XPS analyses. Cellular internalization of nanoparticles was studied by inverted light scattering microscopy, and cytotoxicity was determined by XTT analysis. Results demonstrated that the synthesized DcMNPs, with their functional groups, symmetry perfection, size distribution, improved magnetic properties, and nontoxic characteristics could be suitable nanocarriers for targeted cancer therapy upon loading with various anticancer agents.

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Abbreviations

MNPs:

Magnetic nanoparticles, magnetite, Fe3O4

DcMNPs:

Polyamidoamine (PAMAM) dendrimer-coated magnetic nanoparticles (DcMNPs)

APTS:

Aminopropyltrimethoxysilane

XRD:

X-ray diffraction

FTIR:

Fourier transform infrared spectroscopy

TEM:

Transmission electron microscopy

DLS:

Dynamic light scattering

TGA:

Thermal gravimetric analysis

VSM:

Vibrating sample magnetometry

MS :

Saturated magnetization

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Acknowledgments

The support of Asst. Prof. Dr. Bora Mavis for FTIR, as well as financial support by TÜBİTAK-2215 (PhD Fellowship for foreign citizens), is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Middle East Technical University, 06800, Ankara, Turkey

    Rouhollah Khodadust, Gozde Unsoy & Ufuk Gunduz

  2. Department of Food Engineering, Ahi Evran University, 40100, Kırşehir, Turkey

    Serap Yalcın

  3. Department of Chemical Engineering, Middle East Technical University, 06800, Ankara, Turkey

    Gungor Gunduz

  4. Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey

    Rouhollah Khodadust & Ufuk Gunduz

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  1. Rouhollah Khodadust
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  2. Gozde Unsoy
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Correspondence to Rouhollah Khodadust or Ufuk Gunduz.

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Khodadust, R., Unsoy, G., Yalcın, S. et al. PAMAM dendrimer-coated iron oxide nanoparticles: synthesis and characterization of different generations. J Nanopart Res 15, 1488 (2013). https://doi.org/10.1007/s11051-013-1488-6

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