Abstract
The nanotoxicology as a new subdiscipline of nanotechnology needs to be studied in vivo. To do so, it is essential to understand certain pharmacological information of the nanoparticles in vivo. Silica nanoparticles (SiNPs) have been developed for a number of biomedical uses; however, research on their tissular localization and excretion has been limited. In this study, we analyzed the localization of intravenously administered SiNPs with sizes of 20 and 80 nm in liver and spleen and quantitatively investigated the excretion of SiNPs through urine and feces. The results of the tissular localization study showed that the SiNPs were located in liver evenly; however, they were mainly accumulated in the white pulp of spleen. The quantitative excretory assay found the renal excretion being the main excretion pathway of SiNPs and indicated that the accumulated excretory rate of 80 nm SiNPs through urine was higher than that of 20 nm SiNPs because of the higher hemoconcentration. Further analysis of radioactive substances in the excreta showed the convincing confirmatory evidence that the SiNPs of both the sizes of 20 and 80 nm could be excreted through urine. These results provide important information on in vivo distribution and excretion of SiNPs.
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Acknowledgments
This work was supported by grants from Shanghai Sci-Tech Committee Foundation (no. 0752nm026 & 08DZ2291600), Shanghai Leading Academic Discipline Project (no. S30206) and Zhejiang Provincial Natural Science Foundation of China (no. Y4110665). The authors would like to acknowledge Professor Liyi Shi for kindly providing the SiNPs and helpful discussions during the early stage of this investigation.
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Xie, G., Sun, J. & Zhong, G. Tissular localization and excretion of intravenously administered silica nanoparticles of different sizes. J Nanopart Res 14, 671 (2012). https://doi.org/10.1007/s11051-011-0671-x
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DOI: https://doi.org/10.1007/s11051-011-0671-x