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Ratiometric fluorescent nanoparticles for sensing temperature

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Abstract

A ratiometric type of fluorescent nanoparticle was prepared via an encapsulation–reprecipitation method. By introducing an alkoxysilanized dye as a reference, the nanoparticles (NPs) give both a green and a red fluorescence under one single-wavelength excitation. The resulted ratiometric fluorescence is found to be highly temperature-dependent in the physiological range (25–45 °C), with an intensity temperature sensitivity of −4.0%/°C. Given the small size (20–30 nm in diameter) and biocompatible nature (silica out layer), such kind of NPs were very promising as temperature nanosensors for cellular sensing and imaging.

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Acknowledgments

This study was sponsored by the Alexander-von-Humboldt Foundation (Bonn) and National Natural Science Foundation of China (Grant Nos 10774012 and 10874014).

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Authors and Affiliations

  1. Key Laboratory of Luminescence and Optical Information, Ministry of Education, Institute of Optoelectronic Technology, Beijing Jiaotong University, 100044, Beijing, China

    Hong-Shang Peng & Shi-Hua Huang

  2. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany

    Otto S. Wolfbeis

Authors
  1. Hong-Shang Peng
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  2. Shi-Hua Huang
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  3. Otto S. Wolfbeis
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Correspondence to Hong-Shang Peng.

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Peng, HS., Huang, SH. & Wolfbeis, O.S. Ratiometric fluorescent nanoparticles for sensing temperature. J Nanopart Res 12, 2729–2733 (2010). https://doi.org/10.1007/s11051-010-0046-8

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  • DOI: https://doi.org/10.1007/s11051-010-0046-8

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