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First Environmental Isolations of Cryptococcus neoformans and Cryptococcus gattii in Tunisia and Review of Published Studies on Environmental Isolations in Africa

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Abstract

Cryptococcus neoformans and Cryptococcus gattii are pathogenic yeasts that cause cryptococcosis. These fungi were commonly associated with pigeon droppings and plant materials. The habitat of these pathogens has not been yet studied in Tunisia, although the ecology of these yeasts must be elucidated in order to establish surveillance programs and to prevent infections. The aim of this survey was to recover C. neoformans and C. gattii environmental isolates from pigeon droppings and plant materials in different areas of Sfax region, Tunisia. Nine hundred and fifty samples from leaves, wood, flowers, fruits and soil around trunk bases of 40 almond (Prunus dulcis) and 60 eucalyptus trees were collected as well as 250 pigeon droppings samples from different sites: buildings (n = 150), houses (n = 50) and zoo (n = 50). The identification of Cryptococcus neoformans complex was confirmed using the ID32C auxanogram panel (BioMérieux, Marcy l’Etoile, France); species were determined by multiplex PCR using the CN70 and CN49 primers, and mating type was determined by PCR. C. neoformans was recovered from 26 specimens of pigeon droppings (10.4%). This yeast was obtained more frequently from dry droppings (9.2%) than from moist droppings (1.2%). The mating type was determined. All the 31 environmental strains of C. neoformans and C. gattii were MATα. Out of 700 samples tested from 100 trees, only 5 isolates of Cryptococcus neoformans species complex were recovered (0.6%), two isolates of C. gattii and one isolate of C. neoformans were recovered from the wood of E. camaldulensis trees, and only two isolates of C. gattii were recovered from the wood of almond trees (Prunus dulcis Mill. var. zaaf and var. achek). These two Tunisian almond tree varieties were recorded for the first time in Africa as hosts for C. gattii. These results add new information to the ecology and epidemiology of C. neoformans species complex in Tunisia.

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References

  1. Perfect JR, Casadevall A. Cryptococcosis. Infect Dis Clin North Am. 2002;16:837–74. V–vi.

    Article  PubMed  Google Scholar 

  2. Kwon-Chung KJ, Varma A. Do major species concepts support one, two or more species within Cryptococcus neoformans? FEMS Yeast Res. 2006;6:574–87.

    Article  PubMed  CAS  Google Scholar 

  3. Emmons CW. Saprophytic sources of Cryptococcus neoformans associated with the pigeon (Columba livia). Am J Hyg. 1955;62:227–32.

    PubMed  CAS  Google Scholar 

  4. Casadevall A PJ. Cryptococcus neoformans. ASM press. 1998.

  5. Kwon-Chung KJ, Bennett JE. Epidemiologic differences between the two varieties of Cryptococcus neoformans. Am J Epidemiol. 1984;120:123–30.

    PubMed  CAS  Google Scholar 

  6. Springer DJ, Chaturvedi V. Projecting global occurrence of Cryptococcus gattii. Emerg Infect Dis. 2010;16:14–20.

    Article  PubMed  Google Scholar 

  7. Ellis DH, Pfeiffer TJ. Natural habitat of Cryptococcus neoformans var. gattii. J Clin Microbiol. 1990;28:1642–4.

    PubMed  CAS  Google Scholar 

  8. Chakrabarti A, Jatana M, Kumar P, Chatha L, Kaushal A, Padhye AA. Isolation of Cryptococcus neoformans var gattii from Eucalyptus camaldulensis in India. J Clin Microbiol. 1997;35:3340–2.

    PubMed  CAS  Google Scholar 

  9. Licea BG, Garza DG, Zúñiga MT. Aislamento de Cryptococcus neoformans var. gattii de Eucalyptus tereticornis. Rev Iberoam Micol. 1996;13:27–8.

    Google Scholar 

  10. Callejas A, Ordonez N, Rodriguez MC, Castaneda E. First isolation of Cryptococcus neoformans var gattii, serotype C, from the environment in Colombia. Med Mycol. 1998;36:341–4.

    PubMed  CAS  Google Scholar 

  11. Lazera MS, Salmito Cavalcanti MA, Londero AT, Trilles L, Nishikawa MM, Wanke B. Possible primary ecological niche of Cryptococcus neoformans. Med Mycol. 2000;38:379–83.

    PubMed  CAS  Google Scholar 

  12. Fortes ST, Lazera MS, Nishikawa MM, Macedo RC, Wanke B. First isolation of Cryptococcus neoformans var. gattii from a native jungle tree in the Brazilian Amazon rainforest. Mycoses. 2001;44:137–40.

    Article  PubMed  CAS  Google Scholar 

  13. Staib F, Senska M. Brown pigmentation (brown colour effect, BCE) of Cryptococcus neoformans on Guizotia abyssinica-creatinine-agar as dependent on the initial pH value (author’s transl). Zentralbl Bakteriol Orig A. 1973;225:113–24.

    PubMed  CAS  Google Scholar 

  14. Bolano A, Stinchi S, Preziosi R, et al. Rapid methods to extract DNA and RNA from Cryptococcus neoformans. FEMS Yeast Res. 2001;1:221–4.

    PubMed  CAS  Google Scholar 

  15. Aoki FH, Imai T, Tanaka R, et al. New PCR primer pairs specific for Cryptococcus neoformans serotype A or B prepared on the basis of random amplified polymorphic DNA fingerprint pattern analyses. J Clin Microbiol. 1999;37:315–20.

    PubMed  CAS  Google Scholar 

  16. Chaturvedi S, Rodeghier B, Fan J, McClelland CM, Wickes BL, Chaturvedi V. Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies. J Clin Microbiol. 2000;38:2007–9.

    PubMed  CAS  Google Scholar 

  17. Ellis D, Pfeiffer T. The ecology of Cryptococcus neoformans. Eur J Epidemiol. 1992;8:321–5.

    Article  PubMed  CAS  Google Scholar 

  18. Mahmoud YA. First environmental isolation of Cryptococcus neoformans var. neoformans and var. gatti from the Gharbia Governorate, Egypt. Mycopathologia. 1999;148:83–6.

    Article  PubMed  CAS  Google Scholar 

  19. Escandon P, Sanchez A, Martinez M, Meyer W, Castaneda E. Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia. FEMS Yeast Res. 2006;6:625–35.

    Article  PubMed  CAS  Google Scholar 

  20. Kidd SE, Chow Y, Mak S, et al. Characterization of environmental sources of the human and animal pathogen Cryptococcus gattii in British Columbia, Canada, and the Pacific Northwest of the United States. Appl Environ Microbiol. 2007;73:1433–43.

    Article  PubMed  CAS  Google Scholar 

  21. Granados DP, Castaneda E. Isolation and characterization of Cryptococcus neoformans varieties recovered from natural sources in Bogota, Colombia, and study of ecological conditions in the area. Microb Ecol. 2005;49:282–90.

    Article  PubMed  CAS  Google Scholar 

  22. Nishikawa MM, Lazera MS, Barbosa GG, et al. Serotyping of 467 Cryptococcus neoformans isolates from clinical and environmental sources in Brazil: analysis of host and regional patterns. J Clin Microbiol. 2003;41:73–7.

    Article  PubMed  Google Scholar 

  23. Kuroki M, Phichaichumpon C, Yasuoka A, et al. Environmental isolation of Cryptococcus neoformans from an endemic region of HIV-associated cryptococcosis in Thailand. Yeast. 2004;21:809–12.

    Article  PubMed  CAS  Google Scholar 

  24. Baroni Fde A, Paula CR, Silva EG, et al. Cryptococcus neoformans strains isolated from church towers in Rio de Janeiro City, RJ, Brazil. Rev Inst Med Trop Sao Paulo. 2006;48:71–5.

    PubMed  Google Scholar 

  25. Littman ML, Borok R. Relation of the pigeon to cryptococcosis: natural carrier state, heat resistance and survival of Cryptococcus neoformans. Mycopathol Mycol Appl. 1968;35:329–45.

    Article  PubMed  CAS  Google Scholar 

  26. Ruiz A, Fromtling RA, Bulmer GS. Distribution of Cryptococcus neoformans in a natural site. Infect Immun. 1981;31:560–3.

    PubMed  CAS  Google Scholar 

  27. Kidd SE, Hagen F, Tscharke RL, et al. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc Natl Acad Sci USA. 2004;101:17258–63.

    Article  PubMed  CAS  Google Scholar 

  28. Kwon-Chung KJ. A new species of Filobasidiella, the sexual state of Cryptococcus neoformans B and C serotypes. Mycologia. 1976;68:943–6.

    PubMed  CAS  Google Scholar 

  29. Refai M, Taha M, Selim SA, Elshabourii F, Yousseff HH. Isolation of Cryptococcus neoformans, Candida albicans and other yeasts from pigeon droppings in Egypt. Sabouraudia. 1983;21:163–5.

    Article  PubMed  CAS  Google Scholar 

  30. Swinne D, Deppner M, Laroche R, Floch JJ, Kadende P. Isolation of Cryptococcus neoformans from houses of AIDS-associated cryptococcosis patients in Bujumbura (Burundi). Aids. 1989;3:389–90.

    Article  PubMed  CAS  Google Scholar 

  31. Swinne D, Taelman H, Batungwanayo J, Bigirankana A, Bogaerts J. Ecology of Cryptococcus neoformans in central Africa. Med Trop (Mars). 1994;54:53–5.

    CAS  Google Scholar 

  32. Irokanulo EO, Makinde AA, Akuesgi CO, Ekwonu M. Cryptococcus neoformans var neoformans isolated from droppings of captive birds in Nigeria. J Wildl Dis. 1997;33:343–5.

    PubMed  CAS  Google Scholar 

  33. Laurenson IF, Lalloo DG, Naraqi S, et al. Cryptococcus neoformans in Papua New Guinea: a common pathogen but an elusive source. J Med Vet Mycol. 1997;35:437–40.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de, 3029, Sfax, Tunisia

    F. Mseddi, A. Sellami, M. A. Jarboui, H. Sellami, F. Makni & A. Ayadi

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  1. F. Mseddi
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  2. A. Sellami
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  3. M. A. Jarboui
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  4. H. Sellami
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Correspondence to A. Ayadi.

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Mseddi, F., Sellami, A., Jarboui, M.A. et al. First Environmental Isolations of Cryptococcus neoformans and Cryptococcus gattii in Tunisia and Review of Published Studies on Environmental Isolations in Africa. Mycopathologia 171, 355–360 (2011). https://doi.org/10.1007/s11046-010-9381-7

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  • DOI: https://doi.org/10.1007/s11046-010-9381-7

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