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Isolation and Characterization of Cryptococcus neoformans Varieties Recovered from Natural Sources in Bogotá, Colombia, and Study of Ecological Conditions in the Area

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Abstract

Cryptococcus neoformans, the etiological agent of cryptococcosis, has been associated with avian droppings and certain trees in different countries, including Colombia. C neoformans environmental isolates were obtained in urban areas in Bogotá, Colombia, and the strains recovered were phenotypically characterized. Attempts to determine the ecological conditions (micro- and macroclimatic) possibly related to their habitat were also undertaken. Four hundred and eighty samples from bark, soil around trunk bases, and detritus inside hollows of 32 trees were collected in three urban areas during a 5-month period, as well as 89 avian droppings samples from different places. Of plant samples, 6.7% collected from nine tree species yielded C. neoformans var. gattii, serotype B strains in 99% of the cases, and C. neoformans var. grubii, serotype A in 1%. The yeast was more frequently recovered from bark than from soil or detritus inside hollows, and from trees with hollows or rotted wood rather than from trees in which birds nest. C. neoformans was present with higher frequency and density in the rainy season than in the dry season; we found that slightly higher temperature and humidity values of the microhabitat, as compared to those of the environment, favored fungal occurrence, but the phenological state of the tree did not. Of dropping samples, 7.9% yielded C. neoformans strains, all of them C. neoformans var. grubii, serotype A. The yeast was obtained more frequently from dry droppings than from moist ones, but neither the sunlight exposure nor the site of collection of samples was correlated with this occurrence. Population density was significantly higher in droppings than in tree samples. Under laboratory conditions, isolates of different serotype showed similar capsular sizes. Water content and pH ranges were wide and did not show any significant difference between positive and negative samples.

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References

  1. E Adams B Cooper (1974) ArticleTitleEvaluation of a modified Wickerman medium for identifying medically important yeast Am J Med Technol 40 377–388 Occurrence Handle4413293

    PubMed  Google Scholar 

  2. LF Cabral Passoni (1999) ArticleTitleWood, animals and human beings as reservoirs for human Cryptococcus neoformans infection Rev Iberoam Micol 16 77–81

    Google Scholar 

  3. LD Caicedo MI Álvarez M Delgado A Cárdenas (1999) ArticleTitleCryptococcus neoformans in bird excreta in the city zoo of Cali, Colombia Mycopathologia 147 121–124 Occurrence Handle10.1023/A:1007146700339 Occurrence Handle11040862

    Article  PubMed  Google Scholar 

  4. A Callejas N Ordóñez MC Rodríguez E Castañeda (1998) ArticleTitleFirst isolation of Cryptococcus neoformans var. gattii, serotype C from the environment in Colombia Med Mycol 36 341–344 Occurrence Handle10.1046/j.1365-280X.1998.00159.x Occurrence Handle10075505

    Article  PubMed  Google Scholar 

  5. A Casadevall JR Perfect (1998) Cryptococcus neoformans American Society for Microbiology Press Washington, DC

    Google Scholar 

  6. E Castañeda (2001) ArticleTitleEn búsqueda del hábitat de Cryptococcus neoformans var. gattii en Colombia Rev Acad Colomb Cienc 25 105–114

    Google Scholar 

  7. JH Connolly MB Krockenberger R Malik J Canfield DI Wigney DB Muir (1999) ArticleTitleAsymptomatic carriage of Cryptococcus neoformans in the nasal cavity of the koala (Phascolarctos cinereus) Med Mycol 37 331–338 Occurrence Handle10.1046/j.1365-280X.1999.00236.x Occurrence Handle10520157

    Article  PubMed  Google Scholar 

  8. CS Corrales N Ordóñez LM Londoño E Castañeda (1981) ArticleTitleDeterminación de anticuerpos contra Cryptococcus neoformans en un grupo de colombófilos Biomédica 7 100–104

    Google Scholar 

  9. A Duarte N Ordóñez E Castañeda (1994) ArticleTitleAsociación de levaduras del género Cryptococcus con especies de Eucalyptus en Santafé de Bogotá Rev Inst Med Trop Sao Paulo 36 125–130 Occurrence Handle7997787

    PubMed  Google Scholar 

  10. DH Ellis TJ Pfeiffer (1990) ArticleTitleNatural habitat of Cryptococcus neoformans var. gattii J Clin Microbiol 28 1642–1644 Occurrence Handle2199524

    PubMed  Google Scholar 

  11. SP Franzot J Mukherjee R Cherniak L Chien JS Hamdan A Casadevall (1998) ArticleTitleMicroevolution of a standard strain of Cryptococcus neoformans resulting in differences in virulence and other phenotypes Infect Immun 66 89–97 Occurrence Handle9423844

    PubMed  Google Scholar 

  12. SP Franzot IF Salkin A Casadevall (1999) ArticleTitleCryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates J Clin Microbiol 37 838–840 Occurrence Handle9986871

    PubMed  Google Scholar 

  13. AJ Hamilton J Goodley (1996) ArticleTitleVirulence factors of Cryptococcus neoformans Curr Top Med Mycol 7 19–42 Occurrence Handle9504057

    PubMed  Google Scholar 

  14. RA Johnson DW Wichern (2002) Applied Multivariate Statistical Analysis Prentice Hall Upper Saddle River, NJ

    Google Scholar 

  15. MB Krockenberger PJ Canfield R Malik (2002) ArticleTitleCryptococcus neoformans in the koala (Phascolarctos cinereus): colonization by Cryptococcus neoformans var. gattii and investigation of environmental sources Med Mycol 40 263–272 Occurrence Handle12146756

    PubMed  Google Scholar 

  16. KJ Kwon-Chung JR Bennett (1982) ArticleTitleImproved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C) J Clin Microbiol 15 535–537 Occurrence Handle7042750

    PubMed  Google Scholar 

  17. KJ Kwon-Chung BL Wickes JL Booth HS Vishniac JE Bennet (1987) ArticleTitleUrease inhibition by EDTA in the two varieties of Cryptococcus neoformans Infect Immun 55 1751–1754 Occurrence Handle3112009

    PubMed  Google Scholar 

  18. KJ Kwon-Chung JE Bennett (1992) Medical Mycology Lea & Febiger Philadelphia

    Google Scholar 

  19. MS Lazera (1996) ArticleTitleNatural habitat of Cryptococcus neoformans var. neoformans in decaying wood forming hollows in living trees J Med Vet Mycol 34 127–131 Occurrence Handle8732358

    PubMed  Google Scholar 

  20. MS Lazera (1998) ArticleTitleCryptococcus neoformans var. gattii — evidence for a natural habitat related to decaying wood in a pottery tree hollow Med Mycol 36 119–122 Occurrence Handle10.1046/j.1365-280X.1998.00120.x

    Article  Google Scholar 

  21. MS Lazera MA Salmito Cavalcanti AT Londero L Trilles MM Nishikawa B Wanke (2000) ArticleTitlePossible primary ecological niche of Cryptococcus neoformans Med Mycol 38 379–383 Occurrence Handle11092385

    PubMed  Google Scholar 

  22. CA Mira R Anzola A Martínez R Llinas C Valencia A Restrepo (1968) ArticleTitleAislamiento de Cryptococcus neoformans a partir de materiales contaminados con excretas de palomas en Medellín, Colombia Antioquia Med 18 33–40

    Google Scholar 

  23. TG Mitchell JR Perfect (1995) ArticleTitleCryptococcosis in the era of AIDS — 100 years after the discovery of Cryptococcus neoformans Clin Microbiol Rev 8 515–548 Occurrence Handle8665468

    PubMed  Google Scholar 

  24. H Montenegro CR Paula (2000) ArticleTitleEnvironmental isolation of Cryptococcus neoformans var. gattii and C. neoformans var. neoformans in the city of Sao Paulo, Brazil Med Mycol 38 385–390 Occurrence Handle11092386

    PubMed  Google Scholar 

  25. N Ordóñez E Castañeda (2001) ArticleTitleVarieties and serotypes of Cryptococcus neoformans clinical isolates in Colombia Rev Iberoam Micol 18 28–130

    Google Scholar 

  26. Pérez A (1996) Perfil ambiental de Santafé de Bogotá. Corporación Misión Siglo XXI, Bogotá, D.C.

  27. HS Randhawa T Kowshik ZU Khan (2003) ArticleTitleDecayed wood of Syzygium cumini and Ficus religiosa living trees in Delhi/New Delhi metropolitan area as natural habitat of Cryptococcus neoformans Med Mycol 41 199–209 Occurrence Handle10.1080/369378031000137251 Occurrence Handle12964711

    Article  PubMed  Google Scholar 

  28. A Ruíz RA Fromtling GS Bulmer (1981) ArticleTitleDistribution of Cryptococcus neoformans in a natural site Infect Immun 31 560–563 Occurrence Handle7012011

    PubMed  Google Scholar 

  29. F Schinner R Ohlinger E Kandeler R Margesin (1996) Methods in Soil Biology Springer-Verlag Berlin

    Google Scholar 

  30. TC Sorrell (2001) ArticleTitleCryptococcus neoformans variety gattii Med Mycol 39 155–168 Occurrence Handle11346263

    PubMed  Google Scholar 

  31. F Staib (1985) ArticleTitleSampling and isolation of Cryptococcus neoformans from indoor air with the aid of the Reuter centrifugal sampler (RCS) and Guizotia abyssinica creatinine agar: a contribution to the mycological-epidemiological control of Cryptococcus neoformans in the fecal matter of caged birds Zentralbl Bakteriol Hyg Abt I Orig B 180 567–575

    Google Scholar 

  32. RGD Steel JH Torrie (1985) Principles and Procedures of Statistics: A Biometrical Approach McGraw-Hill New York

    Google Scholar 

  33. C Stephen S Lester W Black M Fyfe S Raverty (2002) ArticleTitleMultispecies outbreak of cryptococcosis on southern Vancouver Island, British Columbia Can Vet J 43 792–794 Occurrence Handle12395765

    PubMed  Google Scholar 

  34. I Vilcins M Krockenberger A Agus D Carter (2002) ArticleTitleEnvironmental sampling for Cryptococcus neoformans var. gattii from the Blue Mountains National Park, Sydney, Australia Med Mycol 40 53–60 Occurrence Handle11860013

    PubMed  Google Scholar 

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Acknowledgments

We thank Colciencias for financial support (Grant 2104-04-118-02), Dr. Eduardo Granados for sample design and statistical analysis, Professor Jimena Sánchez for comments on the study design and manuscript correction, and Martha Renza for the English correction of the manuscript.

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  1. Grupo de Microbiología, Instituto Nacional de Salud, Avenida calle 26 No 51-60, Bogotá D.C., Colombia

    D.P. Granados & E. Castañeda

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  1. D.P. Granados
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  2. E. Castañeda
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Correspondence to E. Castañeda.

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Granados, D., Castañeda, E. Isolation and Characterization of Cryptococcus neoformans Varieties Recovered from Natural Sources in Bogotá, Colombia, and Study of Ecological Conditions in the Area. Microb Ecol 49, 282–290 (2005). https://doi.org/10.1007/s00248-004-0236-y

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  • DOI: https://doi.org/10.1007/s00248-004-0236-y

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