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Variability of the Mitochondrial SSU rDNA of Nomuraea Species and Other Entomopathogenic Fungi from Hypocreales

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Abstract

Hypocrealean arthropod pathogenic fungi have profound impact on the regulation of agricultural and medical pests. However, until now the genetic and phylogenetic relationships among species have not been clarified, such studies could clarify host specificity relationships and define species boundaries. Our purpose was to compare the sequences of the mitochondrial SSU rDNA fragments from several mitosporic entomopathogenic Hypocreales to infer relationships among them and to evaluate the possibility to use these sequences as species diagnostic tool in addition to the more commonly studied sequences of nuclear SSU rDNA. The SSU mt-rDNA proved to be useful to help in differentiation of species inside several genera. Clusters obtained with Parsimony, Bayesian, and Maximum Likelihood analyses were congruent with a new classification of the Clavicipitaceae (Sung et al. Stud Mycol. 2007;57:5–59) in which the anamorphic genera Nomuraea and Metarhizium species remain in the Clavicipitaceae and Isaria species sequenced here are assigned to the family Cordycipitaceae. Mitochondrial genomic information indicates the same general pattern of relationships demonstrated by nuclear gene sequences.

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Acknowledgments

The study was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) from Brazil and USDA Agricultural Research Service (Ithaca, New York). We wish to acknowledge John Vandenberg for allowing to use his facilities, Drion G. Boucias for the critical review of the manuscript and providing some N. rileyi isolates, Karen Hansen and Louela Castrillo for helping with laboratory work. DRSG thanks CNPq (Project 490348/2004-1 and fellowship 303997/2004–4) and KLSB thanks CNPq (fellowship 151004/2005–6) and Fundação de Amparo à Pesquisa––FAPESP (grants #06/60127–0 and #07/53919–0).

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Authors and Affiliations

  1. Embrapa - National Soybean Research Center, Embrapa Soja, Londrina, Cx. P. 231, 86001-970, PR, Brazil

    Daniel R. Sosa-Gómez & Eliseu Binneck

  2. Biological Integrated Pest Management, Robert W. Holley Center for Agriculture and Health, Tower Road, Ithaca, NY, 14853-2901, USA

    Richard A. Humber

  3. Department of Plant Pathology, Cornell University, Ithaca, NY, 14853, USA

    Kathie T. Hodge

  4. Departamento de Zoologia, Instituto de Biologia, Universidade Estadual de Campinas, Cx. P. 6109, Campinas, 13083-970, SP, Brazil

    Karina Lucas da Silva-Brandão

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  1. Daniel R. Sosa-Gómez
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Correspondence to Daniel R. Sosa-Gómez.

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Sosa-Gómez, D.R., Humber, R.A., Hodge, K.T. et al. Variability of the Mitochondrial SSU rDNA of Nomuraea Species and Other Entomopathogenic Fungi from Hypocreales. Mycopathologia 167, 145–154 (2009). https://doi.org/10.1007/s11046-008-9157-5

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