Abstract
The aim of the present study was to evaluate the antifungal activity of fatty acids against phytopathogenic fungi. Two pot experiments were conducted by mixing palmitic and oleic acids in the soil in which poor plant growth was observed. In addition, the antifungal activities of nine fatty acids (butyric acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, oleic acid, and linoleic acid) against four phytopathogenic fungi: Alternaria solani, Colletotrichum lagenarium, Fusarium oxysporum f. sp. Cucumerinum, and Fusarium oxysporum f. sp. lycopersici, were assessed by measuring mycelial growth and spore germination via Petri dish assay. The results of the pot experiments showed that the mixture of palmitic and oleic acids enhanced the growth of the seedlings of continuous-tomato and continuous-cucumber. Except for oleic acid, in the Petri dish assay, the fatty acids tested were observed to inhibit the mycelial growth of one or more tested fungi. In addition to the suppression of mycelial growth, butyric acid, caproic acid, caprylic acid, capric acid, lauric acid, and palmitic acid showed an inhibitory effect against spore germination and the extent of inhibition varied with both the type of fatty acids, and the fungi. In particular, capric acid displayed strong inhibitory effect against C. lagenarium on the mycelial growth and spore germination. The saturated fatty acids, i.e. palmitic acids, showed stronger antifungal activity than the unsaturated fatty acids, i.e. oleic acid. It suggests that fatty acids might be applicable to exploring for alternative approaches to integrated control of phytopathogens.
Similar content being viewed by others
References
Kabara JJ, Swieczkowski DM, Conley AJ, Truant JP. Fatty acids and derivatives as antimicrobial agents. Antimicrob Agent Chemother. 1972;2:23–8.
Carballeira NM. New advances in fatty acids as antimalarial, antimycobacterial and antifungal agents. Prog Lipid Res. 2008;47:50–61.
Morbidoni HR, Vilcheze C, Kremer L, Bittman R, Sacchettini JC Jr, Jacobs WR. Dual inhibition of mycobacterial fatty acid biosynthesis and degradation by 2-alkynoic acids. Chem Biol. 2006;13:297–307.
Bergsson G, Arnfinnsson J, Steingrimsson O, Thormar H. In vitro killing of Candida albicans by fatty acids and monoglycerides. Antimicrob Agents Chemother. 2001;45:3209–12.
Avis TJ, Bélanger RR. Specificity and mode of action of the antifungal fatty acid cis-9-heptadecenoic acid produced by Pseudozyma flocculosa. Appl Environ Microbiol. 2001;67:956–60.
Walters DR, Walker RL, Walker KC. Lauric acid exhibits antifungal activity against plant pathogenic fungi. J Phytopathol. 2003;151:228–30.
Řiháková Z, Plocková M, Filip V, Šmidrkal J. Antifungalactivity of lauric acid derivatives against Aspergillus niger. Eur Food Res Tech. 2001;213:488–90.
Walters D, Raynor L, Mitchell A, Walker R, Walker K. Antifungal activities of four fatty acids against plant pathogenic fungi. Mycopathologia. 2004;157:87–90.
Duniway JM. Status of chemical alternatives to methyl bromide for pre-plant fumigation of soil. Phytopathology. 2002;92:1337–43.
Ruan WB, Liu MH, Pan J, Wang J, Lu WL, Ma CC, Wang JG, Gao YB. Effect of oil cakes on the growth of cucumber (Cucumis sativus L.) under continuous cropping system and its mechanism. Scientia Agricultura Sinica. 2003;36(12):1519–24.
Ruan WB, Wang J, Pan J, Li HB, Wang JG, Zhang FS, Gao YB. Effects of sesame seed cake allelochemicals on the growth cucumber (Cucumis sativus L. cv. Jinchun 4). Allelopathy J. 2005;16(2):217–25.
Liu XP, Wang JY, Gou P, Mao CG, Zhu ZR, Li HY. In vitro inhibition of postharvest pathogens of fruit and control of gray mold of strawberry and green mold of citrus by aureobasidin A. Int J Food Microbiol. 2007;119:223–9.
Ross WF, Walters DR, Robins DJ. Synthesis and antifungal activity of five classes of diamines. Pest Manag Sci. 2004;60:143–8.
Widmer TL, Laurent N. Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp. pathogenic to Theobroma cacao. Eur J Plant Pathol. 2006;115:377–88.
Karuna R, Sashidhar RB. The mycotoxin fumonisin B1 inhibits eukaryotic protein synthesis: in vitro and in vivo studies. Mycopathologia. 2008;165:37–49.
Agrios GN. Plant pathology. 4th ed. San Francisco: Academic Press; 1997.
Li XC, Jacob MR, ElSohly HN, Nagle DG, Smillie TJ, Walker LA, et al. Acetylenic acids inhibiting azole-resistant Candida albicans from Pentagonia gigantifolia. J Nat Prod. 2003;66:1132–5.
Acknowledgments
We are grateful to Dr. Yaoting Yu (Nankai University, China) and Dr. Senyu Chen (University of Minnesota, USA) for their critical review of this manuscript. We also thank Dr. Zhijin Fan (Nankai University, China) and Dr. Ligang Zhou (China Agricultural University, China) for providing tested fungi and helpful suggestions. This work was jointly supported by Tianjin Natural Science foundation (06YFJMJC121000), National High-tech R&D Program (2006AA10Z423) and National Key Technologies R&D Program (2006BAD07B03).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, S., Ruan, W., Li, J. et al. Biological Control of Phytopathogenic Fungi by Fatty Acids. Mycopathologia 166, 93–102 (2008). https://doi.org/10.1007/s11046-008-9124-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11046-008-9124-1