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Prostaglandin E2 production by high and low virulent strains of Paracoccidioides brasiliensis

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Abstract

The production of prostaglandins (PGs) during fungal infections could be an important suppressor factor of host immune response. Host cells are one source of prostaglandin E2 (PGE2); however another potential source of PGE2 is the fungal pathogen itself. Thus, both host and fungal PGE2 production is theorized to play a role in pathogenesis, being critical for growth of the fungus and to modulate the host immune response. The purpose of this work was to investigate if high and low virulent strains of Paracoccidioides brasiliensis have the capacity to produce PGE2 in vitro, and if this production was related to the fungal growth. The results demonstrated that both strains of P. brasiliensis produce high levels of PGE2 and the treatment with indomethacin, a cyclooxygenase inhibitor, significantly reduced the production of this mediator, as well as the viability of the fungus. Thus, our data indicate that PGE2 is produced by P. brasiliensis by a cyclooxygenase–dependent metabolic pathway, and its production is required for fungal survival. This discovery reveals an important factor that has potentially great implications for understanding the mechanisms of immune deviation during infection.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), Rubião Júnior, CEP: Botucatu, São Paulo, 18618-000, Brazil

    Ana Paula Bordon, Luciane Alarcão Dias-Melicio, Michele Janegitz Acorci, Guilherme Augusto Biondo, Maria Terezinha Serrão Peraçoli & Ângela Maria Victoriano Campos de Soares

  2. Department of Pathology, Medical School, São Paulo State University, Rubião Júnior, CEP: Botucatu, São Paulo, 18618-000, Brazil

    Denise Fecchio

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  1. Ana Paula Bordon
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  2. Luciane Alarcão Dias-Melicio
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Correspondence to Ângela Maria Victoriano Campos de Soares.

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Bordon, A.P., Dias-Melicio, L.A., Acorci, M.J. et al. Prostaglandin E2 production by high and low virulent strains of Paracoccidioides brasiliensis . Mycopathologia 163, 129–135 (2007). https://doi.org/10.1007/s11046-007-0098-1

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